Antimicrobial Screening and Fungicidal Properties of Eucalýptus globulus Ultrasonic Extracts
Abstract
:1. Introduction
2. Results
2.1. Determination of Dry Matter in E. globúlus Extracts
2.2. Study of the Antimicrobial Activity of E. globúlus Extracts
2.3. Study of the Qualitative and Quantitative Composition of BASs in E. globulus Extracts
3. Discussion
4. Materials and Methods
4.1. Object of Research
4.2. Research Methods
4.3. Evaluation of the Quality of Eucalýptus globúlus Leaf Extract
4.3.1. Determination of Dry Residue by Refractometric Method
4.3.2. Method for Determining the Antimicrobial Activity of a Plant Extract
4.3.3. Methodology and Conditions for High-Performance Liquid Chromatography
4.4. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Extraction Method | Extraction Time, min | Microorganism Strain | |||
---|---|---|---|---|---|
Bacillus subtilis | Pseudomonasaeruginosa | Candida albicans | |||
Without ultrasonic treatment | 10 | 6.0 | 11.0 | 10.5 | |
15 | 6.0 | 13.0 | 8.5 | ||
20 | 9.0 | 14.0 | 7.5 | ||
25 | 7.0 | 10.0 | 8.5 | ||
30 | 7.0 | 7.0 | 8.5 | ||
With ultrasonic treatment | 10 | 10.0 | 6.0 | 12.5 | |
15 | 11.0 | 5.0 | 9.5 | ||
20 | 10.0 | 13.0 | 12.5 | ||
25 | 9.0 | 11.0 | 15.5 | ||
30 | 10.0 | 11.0 | 11.0 | ||
Ampicillin | - | 32.5 | 34.0 | 31.0 | |
Fluconazole | - | - | - | 31.0 |
Component | Release Time, min | Extract Samples | ||||
---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | ||
3,4-dihydroxybenzoic acid | 5.980 | 19.16 ± 0.2 a | 28.30 ± 0.2 b | 25.00 ± 0.2 b | 27.55 ± 0.2 b | 21.28 ± 0.2 a |
Catechin | 9.689 | 157.86 ± 1.2 a | 212.64 ± 1.2 b | 209.52 ± 1.2 b | 282.54 ± 1.2 c | 183.19 ± 1.2 d |
Chlorogenic acid | 10.464 | 256.46 ± 1.2 a | 261.88 ± 1.2 a | 294.16 ± 1.2 ab | 342.14 ± 1.2 b | 274.38 ± 1.2 a |
Caffeic acid | 10.727 | 17.96 ± 0.2 a | 17.64 ± 0.2 a | 20.17 ± 0.2 a | 26.54 ± 0.2 b | 22.90 ± 0.2 ab |
Hyperoside | 19.537 | 9.51 ± 0.1 a | 8.90 ± 0.1 a | 16.67 ± 1.2 b | 33.38 ± 0.2 c | 11.26 ± 0.2 a |
Rutin | 19.537 | 16.16 ± 0.2 a | 15.03 ± 0.2 a | 60.13 ± 0.2 b | 69.79 ± 0.1 b | 14.07 ± 0.2 a |
Quercetin-3D-Glycoside | 20.151 | 1246.82 ± 12.3 a | 1281.48 ± 12.3 a | 1477.78 ± 12.3 b | 1703.30 ± 12.3 c | 1350.34 ± 12.3 ab |
Astragalin | 25.398 | 1283.64 ± 12.3 a | 1381.04 ± 12.3 a | 1521.44 ± 12.3 b | 1737.82 ± 12.3 c | 1358.68 ± 12.3 a |
Apigenin | 26.710 | 7.42 ± 0.1 a | 14.26 ± 0.2 b | 16.36 ± 0.2 bc | 20.28 ± 0.2 c | 13.04 ± 0.2 b |
Rosmarinic acid | 28.191 | 26.22 ± 1.2 a | 27.4 ± 0.2 a | 30.56 ± 0.2 ab | 36.39 ± 0.2 b | 27.83 ± 0.2 a |
Component | Release Time, min | Extract Samples | ||||
---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | ||
3,4-dihydroxybenzoic acid | 5.980 | 13.98 ± 0.2 a | 13.25 ± 0.2 a | 13.26 ± 0.2 a | 10.44 ± 0.2 a | 13.39 ± 0.2 a |
Catechin | 9.689 | 87.34 ± 0.2 a | 79.77 ± 0.2 b | 105.09 ± 1.2 c | 95.15 ± 0.2 a | 92.75 ± 0.2 a |
Chlorogenic acid | 10.464 | 136.12 ± 1.2 a | 145.93 ± 1.2 ab | 151.70 ± 1.2 b | 139.67 ± 1.2 a | 151.09 ± 1.2 b |
Caffeic acid | 10.727 | 9.00 ± 0.1 a | 8.56 ± 0.1 a | 10.34 ± 0.2 a | 10.22 ± 0.2 a | 11.32 ± 0.2 a |
Hyperoside | 19.537 | 4.80 ± 0.1 a | 5.26 ± 0.1 a | 5.78 ± 0.1 a | 6.94 ± 0.1 a | 5.94 ± 0.1 a |
Rutin | 19.537 | 9.19 ± 0.1 a | 7.22 ± 0.1 a | 16.09 ± 0.2 b | 8.60 ± 0.1 a | 12.16 ± 0.2 b |
Quercetin-3D-Glycoside | 20.151 | 672.92 ± 1.2 a | 708.34 ± 1.2 a | 845.28 ± 1.2 b | 761.78 ± 1.2 b | 806.94 ± 1.2 b |
Astragalin | 25.398 | 686.00 ± 1.2 a | 692.06 ± 1.2 a | 750.88 ± 1.2 b | 732.50 ± 1.2 ab | 723.80 ± 1.2 ab |
Apigenin | 26.710 | 5.09 ± 0.1 a | 5.48 ± 0.1 a | 3.56 ± 0.1 b | 8.01 ± 0.1 c | 5.05 ± 0.1 a |
Rosmarinic acid | 28.191 | 14.44 ± 0.2 a | 15.18 ± 0.2 a | 16.29 ± 0.2 a | 15.25 ± 0.2 a | 15.20 ± 0.2 a |
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Sukhikh, S.; Ivanova, S.; Babich, O.; Larina, V.; Krol, O.; Prosekov, A.; Popov, A.; Kriger, O. Antimicrobial Screening and Fungicidal Properties of Eucalýptus globulus Ultrasonic Extracts. Plants 2022, 11, 1441. https://doi.org/10.3390/plants11111441
Sukhikh S, Ivanova S, Babich O, Larina V, Krol O, Prosekov A, Popov A, Kriger O. Antimicrobial Screening and Fungicidal Properties of Eucalýptus globulus Ultrasonic Extracts. Plants. 2022; 11(11):1441. https://doi.org/10.3390/plants11111441
Chicago/Turabian StyleSukhikh, Stanislav, Svetlana Ivanova, Olga Babich, Viktoria Larina, Olesia Krol, Alexander Prosekov, Alexander Popov, and Olga Kriger. 2022. "Antimicrobial Screening and Fungicidal Properties of Eucalýptus globulus Ultrasonic Extracts" Plants 11, no. 11: 1441. https://doi.org/10.3390/plants11111441