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Article

Metabolomics Reveals the Allelopathic Potential of the Invasive Plant Eupatorium adenophorum

by 1,2, 3, 4, 5,* and 4,*
1
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China
2
State Key Laboratory of Hydrology-Water Resources and Hydraulic Engineering, Hohai University, Nanjing 210098, China
3
College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212018, China
4
State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China
5
Shandong Provincial Key Laboratory of Water and Soil Conservation and Environmental Protection, College of Resources and Environment, Linyi University, Linyi 276000, China
*
Authors to whom correspondence should be addressed.
Academic Editors: Bousquet-Mélou Anne, James M Mwendwa and Sajid Latif
Plants 2021, 10(7), 1473; https://doi.org/10.3390/plants10071473
Received: 2 June 2021 / Revised: 9 July 2021 / Accepted: 12 July 2021 / Published: 19 July 2021
(This article belongs to the Special Issue Plant–Plant Allelopathic Interactions)
Phytotoxic chemicals produced by alien invasive plants exert inhibitory effects on native species to facilitate their invasiveness. The allelopathic process of invaders has been hypothesized as the “Novel Weapon Hypothesis”. However, this hypothesis has been controversial for decades due to lack of molecular evidence, and the underlying mechanism of allelopathy still remains ambiguous. Herein, we explore the allelopathic mechanisms of Eupatorium adenophorum, a world-widely spread noxious weed, by the methods of laboratory bioassay and metabolomics analyses in the recipient plant, Arabidopsis thaliana. The bioassay revealed significant phytotoxicity of E. adenophorum extracts. A total of 234 metabolites in A. thaliana were detected by Gas Chromatographic−Mass Spectrometric analysis. There were 48, 99 and 94 impacted metabolites in A. thaliana treated by 50, 25 and 12.5% aqueous extracts compared to control. When mapping all the impacted metabolites to the biological pathways in the KEGG (Kyoto Encyclopedia of Genes and Genomes) database, we found mineral absorption, ABC transporters, amino acid biosynthesis, metabolic pathways and biosynthesis of plant secondary metabolites were mainly impacted. Synthesized with partial least-squares discriminate analysis (PLS-DA) results of metabolic profiles in A. thaliana, we found that citrate cycle was suppressed, metabolism of amino acids was disordered and phosphate absorption was inhibited. Subsequent investigation demonstrated that the phosphorus content in A. thaliana tissues exposed in allelopathic extracts was much lower, indicating inhibition of phosphate uptake. Our study revealed by metabolomics approaches that E. adenophorum is an allelopathic species. View Full-Text
Keywords: allelopathy; exotic plant invasion; metabolomics; phosphate uptake; physiological mechanism allelopathy; exotic plant invasion; metabolomics; phosphate uptake; physiological mechanism
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MDPI and ACS Style

Zhu, X.; Yi, Y.; Huang, L.; Zhang, C.; Shao, H. Metabolomics Reveals the Allelopathic Potential of the Invasive Plant Eupatorium adenophorum. Plants 2021, 10, 1473. https://doi.org/10.3390/plants10071473

AMA Style

Zhu X, Yi Y, Huang L, Zhang C, Shao H. Metabolomics Reveals the Allelopathic Potential of the Invasive Plant Eupatorium adenophorum. Plants. 2021; 10(7):1473. https://doi.org/10.3390/plants10071473

Chicago/Turabian Style

Zhu, Xunzhi, Yangmin Yi, Ling Huang, Chi Zhang, and Hua Shao. 2021. "Metabolomics Reveals the Allelopathic Potential of the Invasive Plant Eupatorium adenophorum" Plants 10, no. 7: 1473. https://doi.org/10.3390/plants10071473

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