In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic Huperzia serrata
College of Life Science, Jiangxi Normal University, Nanchang 330022, China
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Authors to whom correspondence should be addressed.
Academic Editor: Filippo Maggi
Plants 2021, 10(6), 1112; https://doi.org/10.3390/plants10061112
Received: 8 May 2021
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Revised: 28 May 2021
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Accepted: 28 May 2021
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Published: 31 May 2021
(This article belongs to the Section Phytochemistry)
Huperzia serrata is a traditional herb and endangered Chinese medicinal material, which has attracted much attention due to its production of Huperzine A (HupA). In vitro propagation of H. serrata is considered a new way to relieve the resource pressure of H. serrata. In this study, three different genotypic wild H. serrata were used for in vitro propagation. Then, the antioxidant activity and the content of HupA in the regenerated H. serrata were investigated. The results showed the survival rate of the explant was increased to 25.37% when using multiple sterilization processes. The best induction medium for H. serrata was the Schenk and Hildebrandt (SH) medium supplemented with 0.5 mg·L−1 Naphthalene acetic acid (NAA) and 0.1 mg·L−1 2,4-Dichlorophenoxyacetic acid (2,4-D), where the regeneration rate of the explant was to 57.04%. The best proliferation medium was the SH medium with NAA (1.0 mg·L−1), as the biomass of in vitro tissue increased 164.17 ± 0.41 times. High-performance liquid chromatography analysis showed that the in vitro culture of three genotypes could produce HupA and the content of HupA was 53.90–87.17 µg·g−1. The antioxidant experiment showed that the methanol extract of in vitro H. serrata had higher antioxidant activity than that of wild H. serrata. This study provides a reliable in vitro H. serrata culture protocol and laid an important foundation for the antioxidant capacity of the thallus and the content of HupA.
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Keywords:
genotypes; Huperzia serrata; Huperzine A; micropropagation; sterilization; antioxidant
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MDPI and ACS Style
Yang, Y.; Dai, L.; Wu, D.; Dong, L.; Tu, Y.; Xie, J.; Luo, X. In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic Huperzia serrata. Plants 2021, 10, 1112. https://doi.org/10.3390/plants10061112
AMA Style
Yang Y, Dai L, Wu D, Dong L, Tu Y, Xie J, Luo X. In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic Huperzia serrata. Plants. 2021; 10(6):1112. https://doi.org/10.3390/plants10061112
Chicago/Turabian StyleYang, Yan, Liangfang Dai, Decai Wu, Limin Dong, Yisheng Tu, Jiankun Xie, and Xiangdong Luo. 2021. "In Vitro Propagation, Huperzine A Content and Antioxidant Activity of Three Genotypic Huperzia serrata" Plants 10, no. 6: 1112. https://doi.org/10.3390/plants10061112
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