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Article

Echinacea purpurea L. (Moench) Hemagglutinin Effect on Immune Response In Vivo

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Faculty of Pharmacy, Institute of Pharmaceutical Technologies, Academy of Medicine, Lithuanian University of Health Sciences, Sukileliu Ave. 13, 50162 Kaunas, Lithuania
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Large Animal Clinic, Faculty of Veterinary, Veterinary Academy, Lithuanian University of Health Sciences, Tilzes Str. 18, 47181 Kaunas, Lithuania
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Kaunas Botanical Garden, Vytautas Magnus University, Z. E. Zilibero Str. 6, 46324 Kaunas, Lithuania
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Department of Veterinary Pathobiology, Faculty of Veterinary, Academy of Veterinary, Lithuanian University of Health Sciences, Tilzes Str. 18, 47181 Kaunas, Lithuania
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Department of Drug Technology and Social Pharmacy, Faculty of Pharmacy, Academy of Medicine, Lithuanian University of Health Sciences, Sukileliu Ave. 13, 50162 Kaunas, Lithuania
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Department of Pharmacognosy, Faculty of Pharmacy, Academy of Medicine, Lithuanian University of Health Sciences, Sukileliu Ave. 13, 50162 Kaunas, Lithuania
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Laboratry of Cardiac Pathology, Institute of Cardiology, Lithuanian University of Health Sciences, Sukileliu Ave. 15, 50162 Kaunas, Lithuania
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Author to whom correspondence should be addressed.
Academic Editor: Sebastian Granica
Plants 2021, 10(5), 936; https://doi.org/10.3390/plants10050936
Received: 29 March 2021 / Revised: 30 April 2021 / Accepted: 6 May 2021 / Published: 7 May 2021
(This article belongs to the Special Issue Trends in Plants Phytochemistry and Bioactivity Analysis)
Echinacea purpurea L. (Moench) is used in traditional and conventional medicine. However, there is lack of data on the biological activities of primary plant metabolite lectins. The aim of our experiment was to find out how lectin LysM (lysine motif), which was previously purified, affects the immune response in vivo. Eight-week-old BALB/c male mice (n = 15) received four weekly 250 μg/kg peritonial injections of purified Echinacea purpurea L. (Moench) roots’ LysM lectin. The control animal group (n = 15) received 50 μL peritoneal injections of fresh Echinacea purpurea L. (Moench) root tincture, and the negative control animal group (n = 15) received 50 μL peritoneal injections of physiological solution. At the fifth experimental week, the animals were sedated with carbon dioxide, and later euthanized by cervical dislocation, and then their blood and spleen samples were collected. The leukocytes’ formula and lymphocytes’ count was estimated in blood samples, the T lymphocytes’ density was evaluated in spleen zones. A statistically significant (p < 0.05) difference between each group was observed in the leukocytes’ formula (monocytes’ percentage, also little, medium and giant size lymphocytes). The purple coneflower fresh roots’ tincture significantly decreased (p < 0.05) the T lymphocytes’ quantity in peritoneal lymphoid sheaths (PALS) compared with the physiological solution injection’s group (p < 0.05) and the lectin injection’s group (p < 0.001). Meanwhile, lectin injections caused a significant (p < 0.01) increase in the T lymphocytes in a spleen PALS zone, compared with the physiological solution and tincture injection’s group. Our data suggests that LysM lectin acts as an immunostimulant, while fresh purple coneflower tincture causes immunosuppression. View Full-Text
Keywords: Echinacea purpurea L. (Moench); lectin; hemagglutinin; LysM; immune response; T lymphocytes; spleen; blood cells Echinacea purpurea L. (Moench); lectin; hemagglutinin; LysM; immune response; T lymphocytes; spleen; blood cells
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MDPI and ACS Style

Balčiūnaitė-Murzienė, G.; Miknienė, Z.; Ragažinskienė, O.; Juodžiukynienė, N.; Savickas, A.; Savickienė, N.; Pangonytė, D. Echinacea purpurea L. (Moench) Hemagglutinin Effect on Immune Response In Vivo. Plants 2021, 10, 936. https://doi.org/10.3390/plants10050936

AMA Style

Balčiūnaitė-Murzienė G, Miknienė Z, Ragažinskienė O, Juodžiukynienė N, Savickas A, Savickienė N, Pangonytė D. Echinacea purpurea L. (Moench) Hemagglutinin Effect on Immune Response In Vivo. Plants. 2021; 10(5):936. https://doi.org/10.3390/plants10050936

Chicago/Turabian Style

Balčiūnaitė-Murzienė, Gabrielė, Zoja Miknienė, Ona Ragažinskienė, Nomeda Juodžiukynienė, Arūnas Savickas, Nijolė Savickienė, and Dalia Pangonytė. 2021. "Echinacea purpurea L. (Moench) Hemagglutinin Effect on Immune Response In Vivo" Plants 10, no. 5: 936. https://doi.org/10.3390/plants10050936

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