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Volume 10
Issue 3
10.3390/plants10030452
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Article

The Diverse Salt-Stress Response of Arabidopsis ctr1-1 and ein2-1 Ethylene Signaling Mutants Is Linked to Altered Root Auxin Homeostasis

by
Irina I. Vaseva
1,*,
Kiril Mishev
1,
Thomas Depaepe
2,
Valya Vassileva
1 and
Dominique Van Der Straeten
2
1
Department of Molecular Biology and Genetics, Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Str., Bldg. 21, 1113 Sofia, Bulgaria
2
Laboratory of Functional Plant Biology, Department of Biology, Ghent University, K.L. Ledeganckststraat 35, B-9000 Ghent, Belgium
*
Author to whom correspondence should be addressed.
Plants 2021, 10(3), 452; https://doi.org/10.3390/plants10030452
Submission received: 30 January 2021 / Revised: 21 February 2021 / Accepted: 24 February 2021 / Published: 27 February 2021
(This article belongs to the Special Issue Ethylene Signaling and Crosstalk in Plant Responses to Abiotic Stress)
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Abstract

We explored the interplay between ethylene signals and the auxin pool in roots exposed to high salinity using Arabidopsisthaliana wild-type plants (Col-0), and the ethylene-signaling mutants ctr1-1 (constitutive) and ein2-1 (insensitive). The negative effect of salt stress was less pronounced in ctr1-1 individuals, which was concomitant with augmented auxin signaling both in the ctr1-1 controls and after 100 mM NaCl treatment. The R2D2 auxin sensorallowed mapping this active auxin increase to the root epidermal cells in the late Cell Division (CDZ) and Transition Zone (TZ). In contrast, the ethylene-insensitive ein2-1 plants appeared depleted in active auxins. The involvement of ethylene/auxin crosstalk in the salt stress response was evaluated by introducing auxin reporters for local biosynthesis (pTAR2::GUS) and polar transport (pLAX3::GUS, pAUX1::AUX1-YFP, pPIN1::PIN1-GFP, pPIN2::PIN2-GFP, pPIN3::GUS) in the mutants. The constantly operating ethylene-signaling pathway in ctr1-1 was linked to increased auxin biosynthesis. This was accompanied by a steady expression of the auxin transporters evaluated by qRT-PCR and crosses with the auxin transport reporters. The results imply that the ability of ctr1-1 mutant to tolerate high salinity could be related to the altered ethylene/auxin regulatory loop manifested by a stabilized local auxin biosynthesis and transport.
Keywords: Arabidopsis ctr1-1 and ein2-1 mutants; auxin homeostasis; ethylene signals; crosstalk; salt stress Arabidopsis ctr1-1 and ein2-1 mutants; auxin homeostasis; ethylene signals; crosstalk; salt stress
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MDPI and ACS Style

Vaseva, I.I.; Mishev, K.; Depaepe, T.; Vassileva, V.; Van Der Straeten, D. The Diverse Salt-Stress Response of Arabidopsis ctr1-1 and ein2-1 Ethylene Signaling Mutants Is Linked to Altered Root Auxin Homeostasis. Plants 2021, 10, 452. https://doi.org/10.3390/plants10030452

AMA Style

Vaseva II, Mishev K, Depaepe T, Vassileva V, Van Der Straeten D. The Diverse Salt-Stress Response of Arabidopsis ctr1-1 and ein2-1 Ethylene Signaling Mutants Is Linked to Altered Root Auxin Homeostasis. Plants. 2021; 10(3):452. https://doi.org/10.3390/plants10030452

Chicago/Turabian Style

Vaseva, Irina I., Kiril Mishev, Thomas Depaepe, Valya Vassileva, and Dominique Van Der Straeten. 2021. "The Diverse Salt-Stress Response of Arabidopsis ctr1-1 and ein2-1 Ethylene Signaling Mutants Is Linked to Altered Root Auxin Homeostasis" Plants 10, no. 3: 452. https://doi.org/10.3390/plants10030452

APA Style

Vaseva, I. I., Mishev, K., Depaepe, T., Vassileva, V., & Van Der Straeten, D. (2021). The Diverse Salt-Stress Response of Arabidopsis ctr1-1 and ein2-1 Ethylene Signaling Mutants Is Linked to Altered Root Auxin Homeostasis. Plants, 10(3), 452. https://doi.org/10.3390/plants10030452

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