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Article

Boosting of Antioxidants and Alkaloids in Catharanthus roseus Suspension Cultures Using Silver Nanoparticles with Expression of CrMPK3 and STR Genes

1
Botany and Microbiology Department, Faculty of Science, Cairo University, Cairo 12613, Egypt
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Department of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City 32897, Egypt
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Department of Food Science and Nutrition, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
*
Author to whom correspondence should be addressed.
Academic Editors: Iyyakkannu Sivanesan and Katarina Vogel-Mikuš
Plants 2021, 10(10), 2202; https://doi.org/10.3390/plants10102202
Received: 31 August 2021 / Revised: 27 September 2021 / Accepted: 13 October 2021 / Published: 17 October 2021
Global agricultural systems are under unprecedented pressures due to climate change. Advanced nano-engineering can help increase crop yields while ensuring sustainability. Nanotechnology improves agricultural productivity by boosting input efficiency and reducing waste. Alkaloids as one of the numerous secondary metabolites that serve variety of cellular functions essential for physiological processes. This study tests the competence of silver nanoparticles (AgNPs) in boosting alkaloids accumulation in Catharanthus roseus suspension cultures in relation to the expression of C. roseus Mitogen Activated Protein Kinase 3 (CrMPK3) and Strictosidine Synthase (STR) genes. Five concentrations (5, 10, 15, 20 and 25 mg·L−1) of AgNPs were utilized in addition to deionized water as control. Results reflected binary positive correlations among AgNPs concentration, oxidative stress indicated with increase in hydrogen peroxide and malondialdehyde contents, activities of ascorbate peroxidase and superoxide dismutase, expression of the regulatory gene CrMPK3 and the alkaloid biosynthetic gene STR as well as alkaloids accumulation. These correlations add to the growing evidence that AgNPs can trigger the accumulation of alkaloids in plant cells through a signaling pathway that involves hydrogen peroxide and MAPKs, leading to up-regulation of the biosynthetic genes, including STR gene. View Full-Text
Keywords: silver nanoparticles; Catharanthus roseus; H2O2; malondialdehyde; APX; SOD; CrMPK3; STR; alkaloids silver nanoparticles; Catharanthus roseus; H2O2; malondialdehyde; APX; SOD; CrMPK3; STR; alkaloids
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MDPI and ACS Style

Fouad, A.; Hegazy, A.E.; Azab, E.; Khojah, E.; Kapiel, T. Boosting of Antioxidants and Alkaloids in Catharanthus roseus Suspension Cultures Using Silver Nanoparticles with Expression of CrMPK3 and STR Genes. Plants 2021, 10, 2202. https://doi.org/10.3390/plants10102202

AMA Style

Fouad A, Hegazy AE, Azab E, Khojah E, Kapiel T. Boosting of Antioxidants and Alkaloids in Catharanthus roseus Suspension Cultures Using Silver Nanoparticles with Expression of CrMPK3 and STR Genes. Plants. 2021; 10(10):2202. https://doi.org/10.3390/plants10102202

Chicago/Turabian Style

Fouad, Ahmed, Adel E. Hegazy, Ehab Azab, Ebtihal Khojah, and Tarek Kapiel. 2021. "Boosting of Antioxidants and Alkaloids in Catharanthus roseus Suspension Cultures Using Silver Nanoparticles with Expression of CrMPK3 and STR Genes" Plants 10, no. 10: 2202. https://doi.org/10.3390/plants10102202

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