Microcystin-LR, a Cyanobacterial Toxin, Induces DNA Strand Breaks Correlated with Changes in Specific Nuclease and Protease Activities in White Mustard (Sinapis alba) Seedlings
Abstract
:1. Introduction
2. Results
2.1. MC-LR Induces DNA Strand Breaks in Mustard Root Cells as Revealed by the TUNEL Reaction
2.2. MC-LR Induces Changes in Single-Stranded DNase Activities in Mustard Seedlings
2.3. MC-LR Induces Changes in Protease Activities in Mustard Seedlings
3. Discussion
4. Materials and Methods
4.1. The Purification of MC-LR
4.2. Plant Material and MC-LR Treatments
4.3. TUNEL Assay
4.4. Spectrophotometric and In-Gel Activity Assays of Single-Strand Preferring DNase (SSP Nuclease) Activities
4.5. In-Gel Activity Assays of Protease Activities
4.6. Data Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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M-Hamvas, M.; Vasas, G.; Beyer, D.; Nagylaki, E.; Máthé, C. Microcystin-LR, a Cyanobacterial Toxin, Induces DNA Strand Breaks Correlated with Changes in Specific Nuclease and Protease Activities in White Mustard (Sinapis alba) Seedlings. Plants 2021, 10, 2045. https://doi.org/10.3390/plants10102045
M-Hamvas M, Vasas G, Beyer D, Nagylaki E, Máthé C. Microcystin-LR, a Cyanobacterial Toxin, Induces DNA Strand Breaks Correlated with Changes in Specific Nuclease and Protease Activities in White Mustard (Sinapis alba) Seedlings. Plants. 2021; 10(10):2045. https://doi.org/10.3390/plants10102045
Chicago/Turabian StyleM-Hamvas, Márta, Gábor Vasas, Dániel Beyer, Eszter Nagylaki, and Csaba Máthé. 2021. "Microcystin-LR, a Cyanobacterial Toxin, Induces DNA Strand Breaks Correlated with Changes in Specific Nuclease and Protease Activities in White Mustard (Sinapis alba) Seedlings" Plants 10, no. 10: 2045. https://doi.org/10.3390/plants10102045