Metabolomic and Biochemical Analysis of Two Potato (Solanum tuberosum L.) Cultivars Exposed to In Vitro Osmotic and Salt Stresses

Hamooh, Bahget Talat; Sattar, Farooq Abdul; Wellman, Gordon; Mousa, Magdi Ali Ahmed

doi:10.3390/plants10010098

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Metabolomic and Biochemical Analysis of Two Potato (Solanum tuberosum L.) Cultivars Exposed to In Vitro Osmotic and Salt Stresses

¹

Department of Arid Land Agriculture, Faculty of Meteorology, Environment and Arid Land Agriculture, King Abdulaziz University, Jeddah 21589, Saudi Arabia

²

Division of Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Thuwal 23955, Saudi Arabia

³

Department of Vegetables, Faculty of Agriculture, Assiut University, Assiut 71526, Egypt

^*

Authors to whom correspondence should be addressed.

Plants 2021, 10(1), 98; https://doi.org/10.3390/plants10010098

Submission received: 16 December 2020 / Revised: 28 December 2020 / Accepted: 31 December 2020 / Published: 6 January 2021

(This article belongs to the Special Issue Effects of Abiotic Stress on Plants 2020–2021)

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Abstract

Globally, many crop production areas are threatened by drought and salinity. Potato (Solanum tuberosum L.) is susceptible to these challenging environmental conditions. In this study, an in vitro approach was employed to compare the tolerance of potato cultivars ‘BARI-401’ (red skin) and ‘Spunta’ (yellow skin). To simulate ionic and osmotic stress, MS media was supplemented with lithium chloride (LiCl 20 mM) and mannitol (150 mM). GC-MS and spectrophotometry techniques were used to determine metabolite accumulation. Other biochemical properties, such as total phenols concentration (TPC), total flavonoids concentration (TFC), antioxidant capacity (DPPH free radical scavenging capacity), polyphenol oxidase (PPO), and peroxidase (POD) activities, were also measured. The two cultivars respond differently to ionic and osmotic stress treatments, with Spunta accumulating more defensive metabolites in response, indicating a higher level of tolerance. While further investigation of the physiological and biochemical responses of these varieties to drought and salinity is required, the approach taken in this paper provides useful information prior to open field evaluation.

Keywords: drought; salinity; phenols; trehalose; antioxidant; micropropagation; screening

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MDPI and ACS Style

Hamooh, B.T.; Sattar, F.A.; Wellman, G.; Mousa, M.A.A. Metabolomic and Biochemical Analysis of Two Potato (Solanum tuberosum L.) Cultivars Exposed to In Vitro Osmotic and Salt Stresses. Plants 2021, 10, 98. https://doi.org/10.3390/plants10010098

AMA Style

Hamooh BT, Sattar FA, Wellman G, Mousa MAA. Metabolomic and Biochemical Analysis of Two Potato (Solanum tuberosum L.) Cultivars Exposed to In Vitro Osmotic and Salt Stresses. Plants. 2021; 10(1):98. https://doi.org/10.3390/plants10010098

Chicago/Turabian Style

Hamooh, Bahget Talat, Farooq Abdul Sattar, Gordon Wellman, and Magdi Ali Ahmed Mousa. 2021. "Metabolomic and Biochemical Analysis of Two Potato (Solanum tuberosum L.) Cultivars Exposed to In Vitro Osmotic and Salt Stresses" Plants 10, no. 1: 98. https://doi.org/10.3390/plants10010098

APA Style

Hamooh, B. T., Sattar, F. A., Wellman, G., & Mousa, M. A. A. (2021). Metabolomic and Biochemical Analysis of Two Potato (Solanum tuberosum L.) Cultivars Exposed to In Vitro Osmotic and Salt Stresses. Plants, 10(1), 98. https://doi.org/10.3390/plants10010098

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Metabolomic and Biochemical Analysis of Two Potato (Solanum tuberosum L.) Cultivars Exposed to In Vitro Osmotic and Salt Stresses

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