Next Article in Journal
Detecting Mild Water Stress in Olive with Multiple Plant-Based Continuous Sensors
Next Article in Special Issue
Response of Tomato Genotypes under Different High Temperatures in Field and Greenhouse Conditions
Previous Article in Journal
Key Genes in the Melatonin Biosynthesis Pathway with Circadian Rhythm Are Associated with Various Abiotic Stresses
Previous Article in Special Issue
Metabolomic and Biochemical Analysis of Two Potato (Solanum tuberosum L.) Cultivars Exposed to In Vitro Osmotic and Salt Stresses
Open AccessArticle

MicroRNA-Mediated Responses to Cadmium Stress in Arabidopsis thaliana

1
Centre For Plant Science, School of Environmental and Life Sciences, Faculty of Science, University of Newcastle, Callaghan, NSW 2308, Australia
2
Institute of Genome Research, Vietnam Academy of Research and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi 100000, Vietnam
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Plants 2021, 10(1), 130; https://doi.org/10.3390/plants10010130
Received: 24 December 2020 / Revised: 6 January 2021 / Accepted: 7 January 2021 / Published: 10 January 2021
(This article belongs to the Special Issue Effects of Abiotic Stress on Plants 2020–2021)
In recent decades, the presence of cadmium (Cd) in the environment has increased significantly due to anthropogenic activities. Cd is taken up from the soil by plant roots for its subsequent translocation to shoots. However, Cd is a non-essential heavy metal and is therefore toxic to plants when it over-accumulates. MicroRNA (miRNA)-directed gene expression regulation is central to the response of a plant to Cd stress. Here, we document the miRNA-directed response of wild-type Arabidopsis thaliana (Arabidopsis) plants and the drb1, drb2 and drb4 mutant lines to Cd stress. Phenotypic and physiological analyses revealed the drb1 mutant to display the highest degree of tolerance to the imposed stress while the drb2 mutant was the most sensitive. RT-qPCR-based molecular profiling of miRNA abundance and miRNA target gene expression revealed DRB1 to be the primary double-stranded RNA binding (DRB) protein required for the production of six of the seven Cd-responsive miRNAs analyzed. However, DRB2, and not DRB1, was determined to be required for miR396 production. RT-qPCR further inferred that transcript cleavage was the RNA silencing mechanism directed by each assessed miRNA to control miRNA target gene expression. Taken together, the results presented here reveal the complexity of the miRNA-directed molecular response of Arabidopsis to Cd stress. View Full-Text
Keywords: Arabidopsis thaliana (Arabidopsis); cadmium (Cd) stress; microRNA (miRNA); miRNA-directed gene expression regulation; double-stranded RNA binding (DRB) protein; RT-qPCR Arabidopsis thaliana (Arabidopsis); cadmium (Cd) stress; microRNA (miRNA); miRNA-directed gene expression regulation; double-stranded RNA binding (DRB) protein; RT-qPCR
Show Figures

Figure 1

MDPI and ACS Style

Pegler, J.L.; Oultram, J.M.J.; Nguyen, D.Q.; Grof, C.P.L.; Eamens, A.L. MicroRNA-Mediated Responses to Cadmium Stress in Arabidopsis thaliana. Plants 2021, 10, 130. https://doi.org/10.3390/plants10010130

AMA Style

Pegler JL, Oultram JMJ, Nguyen DQ, Grof CPL, Eamens AL. MicroRNA-Mediated Responses to Cadmium Stress in Arabidopsis thaliana. Plants. 2021; 10(1):130. https://doi.org/10.3390/plants10010130

Chicago/Turabian Style

Pegler, Joseph L.; Oultram, Jackson M.J.; Nguyen, Duc Q.; Grof, Christopher P.L.; Eamens, Andrew L. 2021. "MicroRNA-Mediated Responses to Cadmium Stress in Arabidopsis thaliana" Plants 10, no. 1: 130. https://doi.org/10.3390/plants10010130

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop