Next Article in Journal
COL2A1 and Caspase-3 as Promising Biomarkers for Osteoarthritis Prognosis in an Equus asinus Model
Next Article in Special Issue
Connexin 43 Deficiency Is Associated with Reduced Myocardial Scar Size and Attenuated TGFβ1 Signaling after Transient Coronary Occlusion in Conditional Knock-Out Mice
Previous Article in Journal
Traditional Uses, Bioactive Chemical Constituents, and Pharmacological and Toxicological Activities of Glycyrrhiza glabra L. (Fabaceae)
Previous Article in Special Issue
Role of an Aromatic–Aromatic Interaction in the Assembly and Trafficking of the Zebrafish Panx1a Membrane Channel
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biomolecules
Volume 10
Issue 3
10.3390/biom10030353
biomolecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Danegaptide Enhances Astrocyte Gap Junctional Coupling and Reduces Ischemic Reperfusion Brain Injury in Mice

by
Moises Freitas-Andrade
1,
John Bechberger
1,
Jasmine Wang
2,
Ken K.C. Yeung
2,
Shawn N. Whitehead
2,
Rie Shultz Hansen
3 and
Christian C. Naus
1,*
1
Cellular & Physiological Sciences, Faculty of Medicine, Life Science Institute, University of British Columbia, Vancouver, BC V6T 1Z3, Canada
2
Anatomy & Cell Biology, Western University, London, ON N6A 5C1, Canada
3
Zealand Pharma A/S, 2860 Copenhagen, Denmark
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(3), 353; https://doi.org/10.3390/biom10030353
Submission received: 28 December 2019 / Revised: 20 February 2020 / Accepted: 21 February 2020 / Published: 26 February 2020
(This article belongs to the Special Issue Connexins, Innexins, and Pannexins: From Biology to Clinical Targets)
Browse Figures
Versions Notes

Abstract

Ischemic stroke is a complex and devastating event characterized by cell death resulting from a transient or permanent arterial occlusion. Astrocytic connexin43 (Cx43) gap junction (GJ) proteins have been reported to impact neuronal survival in ischemic conditions. Consequently, Cx43 could be a potential target for therapeutic approaches to stroke. We examined the effect of danegaptide (ZP1609), an antiarrhythmic dipeptide that specifically enhances GJ conductance, in two different rodent stroke models. In this study, danegaptide increased astrocytic Cx43 coupling with no significant effects on Cx43 hemichannel activity, in vitro. Using matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI IMS) the presence of danegaptide within brain tissue sections were detected one hour after reperfusion indicating successful transport of the dipeptide across the blood brain barrier. Furthermore, administration of danegaptide in a novel mouse brain ischemia/reperfusion model showed significant decrease in infarct volume. Taken together, this study provides evidence for the therapeutic potential of danegaptide in ischemia/reperfusion stroke.
Keywords: connexin43; stroke; gap junction; astrocytes; danegaptide connexin43; stroke; gap junction; astrocytes; danegaptide

Share and Cite

MDPI and ACS Style

Freitas-Andrade, M.; Bechberger, J.; Wang, J.; Yeung, K.K.C.; Whitehead, S.N.; Shultz Hansen, R.; Naus, C.C. Danegaptide Enhances Astrocyte Gap Junctional Coupling and Reduces Ischemic Reperfusion Brain Injury in Mice. Biomolecules 2020, 10, 353. https://doi.org/10.3390/biom10030353

AMA Style

Freitas-Andrade M, Bechberger J, Wang J, Yeung KKC, Whitehead SN, Shultz Hansen R, Naus CC. Danegaptide Enhances Astrocyte Gap Junctional Coupling and Reduces Ischemic Reperfusion Brain Injury in Mice. Biomolecules. 2020; 10(3):353. https://doi.org/10.3390/biom10030353

Chicago/Turabian Style

Freitas-Andrade, Moises, John Bechberger, Jasmine Wang, Ken K.C. Yeung, Shawn N. Whitehead, Rie Shultz Hansen, and Christian C. Naus. 2020. "Danegaptide Enhances Astrocyte Gap Junctional Coupling and Reduces Ischemic Reperfusion Brain Injury in Mice" Biomolecules 10, no. 3: 353. https://doi.org/10.3390/biom10030353

APA Style

Freitas-Andrade, M., Bechberger, J., Wang, J., Yeung, K. K. C., Whitehead, S. N., Shultz Hansen, R., & Naus, C. C. (2020). Danegaptide Enhances Astrocyte Gap Junctional Coupling and Reduces Ischemic Reperfusion Brain Injury in Mice. Biomolecules, 10(3), 353. https://doi.org/10.3390/biom10030353

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop