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Open AccessArticle

Whole Conversion of Soybean Molasses into Isomaltulose and Ethanol by Combining Enzymatic Hydrolysis and Successive Selective Fermentations

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Marine Science and Engineering College, Qingdao Agricultural University, Qingdao 266109, China
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College of Chemistry & Environmental Engineering, Shandong University of Science & Technology, Qingdao 266510, China
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Development & Reform Bureau, West Coast New Area, Qingdao 266000, China
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Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaiyin Normal University, Huaian 223300, China
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Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(8), 353; https://doi.org/10.3390/biom9080353
Received: 17 July 2019 / Revised: 5 August 2019 / Accepted: 5 August 2019 / Published: 9 August 2019
(This article belongs to the Section Biochemistry)
Isomaltulose is mainly produced from sucrose by microbial fermentation, when the utilization of sucrose contributes a high production cost. To achieve a low-cost isomaltulose production, soy molasses was introduced as an alternative substrate. Firstly, α-galactosidase gene from Rhizomucor miehei was expressed in Yarrowia lipolytica, which then showed a galactosidase activity of 121.6 U/mL. Under the effects of the recombinant α-galactosidase, most of the raffinose-family oligosaccharides in soy molasses were hydrolyzed into sucrose. Then the soy molasses hydrolysate with high sucrose content (22.04%, w/w) was supplemented into the medium, with an isomaltulose production of 209.4 g/L, and the yield of 0.95 g/g. Finally, by virtue of the bioremoval process using Pichia stipitis, sugar byproducts in broth were transformed into ethanol at the end of fermentation, thus resulting in high isomaltulose purity (97.8%). The bioprocess employed in this study provides a novel strategy for low-cost and efficient isomaltulose production from soybean molasses. View Full-Text
Keywords: isomaltulose; soy molasses; raffinose-family oligosaccharides; α-galactosidase; selective fermentation isomaltulose; soy molasses; raffinose-family oligosaccharides; α-galactosidase; selective fermentation
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MDPI and ACS Style

Wang, Z.-P.; Zhang, L.-L.; Liu, S.; Liu, X.-Y.; Yu, X.-J. Whole Conversion of Soybean Molasses into Isomaltulose and Ethanol by Combining Enzymatic Hydrolysis and Successive Selective Fermentations. Biomolecules 2019, 9, 353.

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