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Article

The Integrity of α-β-α Sandwich Conformation Is Essential for a Novel Adjuvant TFPR1 to Maintain Its Adjuvanticity

1
Beijing Institute of Microbiology and Epidemiology, Anhui Medical University, Hefei 230032, China
2
State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing 100071, China
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(12), 869; https://doi.org/10.3390/biom9120869
Received: 7 November 2019 / Revised: 10 December 2019 / Accepted: 11 December 2019 / Published: 12 December 2019
TFPR1 is a novel peptide vaccine adjuvant we recently discovered. To define the structural basis and optimize its application as an adjuvant, we designed three different truncated fragments that have removed dominant B epitopes on TFPR1, and evaluated their capacity to activate bone marrow-derived dendritic cells and their adjuvanticity. Results demonstrated that the integrity of an α-β-α sandwich conformation is essential for TFPR1 to maintain its immunologic activity and adjuvanticity. We obtained a functional truncated fragment TFPR-ta ranging from 40–168 aa of triflin that has similar adjuvanticity as TFPR1 but with 2-log fold lower immunogenicity. These results demonstrated a novel approach to evaluate and improve the activity of protein-based vaccine adjuvant. View Full-Text
Keywords: Pathogenesis-relatedprotein1 (PR-1); conformational structure; adjuvant; dendritic cells (DCs); peptide antigens; B cell epitope Pathogenesis-relatedprotein1 (PR-1); conformational structure; adjuvant; dendritic cells (DCs); peptide antigens; B cell epitope
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MDPI and ACS Style

Li, Q.; Ning, X.; Wang, Y.; Zhu, Q.; Guo, Y.; Li, H.; Zhou, Y.; Kou, Z. The Integrity of α-β-α Sandwich Conformation Is Essential for a Novel Adjuvant TFPR1 to Maintain Its Adjuvanticity. Biomolecules 2019, 9, 869. https://doi.org/10.3390/biom9120869

AMA Style

Li Q, Ning X, Wang Y, Zhu Q, Guo Y, Li H, Zhou Y, Kou Z. The Integrity of α-β-α Sandwich Conformation Is Essential for a Novel Adjuvant TFPR1 to Maintain Its Adjuvanticity. Biomolecules. 2019; 9(12):869. https://doi.org/10.3390/biom9120869

Chicago/Turabian Style

Li, Qiao, Xiuzhe Ning, Yuepeng Wang, Qing Zhu, Yan Guo, Hao Li, Yusen Zhou, and Zhihua Kou. 2019. "The Integrity of α-β-α Sandwich Conformation Is Essential for a Novel Adjuvant TFPR1 to Maintain Its Adjuvanticity" Biomolecules 9, no. 12: 869. https://doi.org/10.3390/biom9120869

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