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Open AccessArticle

Generation of Lamprey Monoclonal Antibodies (Lampribodies) Using the Phage Display System

1
Department of Molecular and Cell Biology, University of California-Merced, Merced, CA 95343, USA
2
U.S. Geological Survey, Western Fisheries Research Center, Seattle, WA 98115, USA
3
Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA 30322, USA
*
Authors to whom correspondence should be addressed.
Biomolecules 2019, 9(12), 868; https://doi.org/10.3390/biom9120868
Received: 1 November 2019 / Revised: 5 December 2019 / Accepted: 9 December 2019 / Published: 12 December 2019
(This article belongs to the Special Issue 2019 Feature Papers by Biomolecules’ Editorial Board Members)
The variable lymphocyte receptors (VLRs) consist of leucine rich repeats (LRRs) and comprise the humoral antibodies produced by lampreys and hagfishes. The diversity of the molecules is generated by stepwise genomic rearrangements of LRR cassettes dispersed throughout the VLRB locus. Previously, target-specific monovalent VLRB antibodies were isolated from sea lamprey larvae after immunization with model antigens. Further, the cloned VLR cDNAs from activated lamprey leukocytes were transfected into human cell lines or yeast to select best binders. Here, we expand on the overall utility of the VLRB technology by introducing it into a filamentous phage display system. We first tested the efficacy of isolating phage into which known VLRB molecules were cloned after a series of dilutions. These experiments showed that targeted VLRB clones could easily be recovered even after extensive dilutions (1 to 109). We further utilized the system to isolate target-specific “lampribodies” from phage display libraries from immunized animals and observed an amplification of binders with relative high affinities by competitive binding. The lampribodies can be individually purified and ostensibly utilized for applications for which conventional monoclonal antibodies are employed. View Full-Text
Keywords: variable lymphocyte receptors; phage display system; library screening; lampribody variable lymphocyte receptors; phage display system; library screening; lampribody
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Hassan, K.M.A.; Hansen, J.D.; Herrin, B.R.; Amemiya, C.T. Generation of Lamprey Monoclonal Antibodies (Lampribodies) Using the Phage Display System. Biomolecules 2019, 9, 868.

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