1. Introduction
2. Results
2.1. Testing the Driver VLRB Molecules in M13 Phage Display System
2.2. Construction and Characterization of a Competitor Phage Display Library
2.3. Subtraction Experiment for Recovery of the Driver Phage by Panning
2.4. Immunization of Lamprey Larvae with Lysozyme and Isolation of Anti-Lysozyme VLRB Clones
2.5. Isolation of VLRB Against Human IgM Protein
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Immunizations
4.3. Naive VLRB Phage Display Library Construction
4.4. Subcloning of Anti-Hen Egg Lysozyme (HEL) VLRB Molecules for Phage Display
4.5. Phage Preparation from the VLRB Phage Display Libraries and the VLRB HEL Phage Display Clones
4.6. Titering the VLRB Phage Display Libraries and Individual VLRB.HEL Phage
4.7. Biotinylation of the Bait and ELISA Screening
4.8. Panning, Rescue, and Superinfection
4.9. ELISA Test of the Candidate Phage Clones
4.10. PCR, Enzyme Digestion and Sequence Analysis of the Phagemid Clones
4.11. Isolation of Bacterially Expressed VLRB Recombinant Proteins, Western Blotting, and Immunohistochemistry (IHC)
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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