Next Article in Journal
Insights into the Chemical Biology of Childhood Embryonal Solid Tumors by NMR-Based Metabolomics
Next Article in Special Issue
Thermal Inactivation of a Cold-Active Esterase PMGL3 Isolated from the Permafrost Metagenomic Library
Previous Article in Journal
Cytotoxic Psammaplysin Analogues from the Verongid Red Sea Sponge Aplysinella Species
Previous Article in Special Issue
The Reverse Side of a Coin: “Factor-Free” Ribosomal Protein Synthesis In Vitro is a Consequence of the In Vivo Proofreading Mechanism
Open AccessReview

Life in Phases: Intra- and Inter- Molecular Phase Transitions in Protein Solutions

1
Department of Molecular Medicine, Morsani College of Medicine, University of South Florida, Tampa, FL 33612, USA
2
Laboratory of New Methods in Biology, Institute for Biological Instrumentation, Russian Academy of Sciences, Federal Research Center “Pushchino Scientific Center for Biological Research of the Russian Academy of Sciences”, 142290 Pushchino, Moscow, Russia
3
Institute of Protein Research, Russian Academy of Sciences, 142290 Pushchino, Moscow, Russia
4
Biology Department, Lomonosov Moscow State University, 119192 Moscow, Russia
5
Bioltechnogy Department, Lomonosov Moscow State University, 142290 Pushchino, Moscow, Russia
*
Authors to whom correspondence should be addressed.
Biomolecules 2019, 9(12), 842; https://doi.org/10.3390/biom9120842
Received: 19 November 2019 / Revised: 5 December 2019 / Accepted: 6 December 2019 / Published: 8 December 2019
Proteins, these evolutionarily-edited biological polymers, are able to undergo intramolecular and intermolecular phase transitions. Spontaneous intramolecular phase transitions define the folding of globular proteins, whereas binding-induced, intra- and inter- molecular phase transitions play a crucial role in the functionality of many intrinsically-disordered proteins. On the other hand, intermolecular phase transitions are the behind-the-scenes players in a diverse set of macrosystemic phenomena taking place in protein solutions, such as new phase nucleation in bulk, on the interface, and on the impurities, protein crystallization, protein aggregation, the formation of amyloid fibrils, and intermolecular liquid–liquid or liquid–gel phase transitions associated with the biogenesis of membraneless organelles in the cells. This review is dedicated to the systematic analysis of the phase behavior of protein molecules and their ensembles, and provides a description of the major physical principles governing intramolecular and intermolecular phase transitions in protein solutions. View Full-Text
Keywords: protein folding; protein structure; intrinsically-disordered protein; molten globule; secondary structure; coil; phase transition; liquid–liquid phase separation; membraneless organelle; amyloid fibril; crystal protein folding; protein structure; intrinsically-disordered protein; molten globule; secondary structure; coil; phase transition; liquid–liquid phase separation; membraneless organelle; amyloid fibril; crystal
Show Figures

Figure 1

MDPI and ACS Style

Uversky, V.N.; Finkelstein, A.V. Life in Phases: Intra- and Inter- Molecular Phase Transitions in Protein Solutions. Biomolecules 2019, 9, 842.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop