Amazon Fruits Inhibit Growth and Promote Pro-apoptotic Effects on Human Ovarian Carcinoma Cell Lines
by
Vanessa Rosse de Souza 1, Mariana Concentino Menezes Brum 2, Isabella dos Santos Guimarães 3, Paula de Freitas dos Santos 2, Thuane Oliveira do Amaral 1, Joel Pimentel Abreu 1
, Thuane Passos 1, Otniel Freitas-Silva 4, Etel Rodrigues Pereira Gimba 2,5 and Anderson Junger Teodoro 1,*
Vanessa Rosse de Souza 1, Mariana Concentino Menezes Brum 2, Isabella dos Santos Guimarães 3, Paula de Freitas dos Santos 2, Thuane Oliveira do Amaral 1, Joel Pimentel Abreu 1, Thuane Passos 1, Otniel Freitas-Silva 4, Etel Rodrigues Pereira Gimba 2,5 and Anderson Junger Teodoro 1,*
1
Laboratory of Functional Foods, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro 22290-240, Brazil
2
Cellular and Molecular Oncobiology Program, Research Centre, Instituto Nacional de Câncer, Rio de Janeiro 20231-050, Brazil
3
Division of Clinical Research, Research Center, Instituto Nacional de Câncer, Rio de Janeiro 20231-050, Brazil
4
Embrapa Agroindústria de Alimentos, Empresa Brasileira de Pesquisa Agropecuária, Rio de Janeiro 23020-470, Brazil
5
Department of Nature Sciences, Universidade Federal Fluminense, Rio das Ostras 28895-532, Brazil
*
Author to whom correspondence should be addressed.
Biomolecules 2019, 9(11), 707; https://doi.org/10.3390/biom9110707
Received: 17 September 2019 / Revised: 25 October 2019 / Accepted: 30 October 2019 / Published: 6 November 2019
(This article belongs to the Special Issue Antitumor Agents from Natural Sources)
Murici (Byrsonima crassifolia (L.) Kunth and B. verbascifolia (L.) DC.) and tapereba (Spondias mombin) are Amazonian fruits that contain bioactive compounds. Biochemical and molecular characterization of these fruits can reveal their potential use in preventing diseases, including cancer. The extracts were characterized regarding the presence and profile of carotenoids by high-performance liquid chromatography (HPLC), total phenolic content by the Folin–Ciocalteu assay, and antioxidant activity by antioxidant value 2,2-diphenyl-1-picrylhydrazyl (DPPH) content analysis, 22,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) content analysis, Ferric-Reducing Ability of Plasma (FRAP), and Oxygen Radical Absorbance Capacity (ORAC) analysis. The extracts of tapereba and murici studied were important sources of total carotenoids and lutein, respectively. The extracts were then tested for their effect on the viability of the A2780 ovarian cancer (OC) cell line and its cisplatin (CDDP)-resistant derived cell line, called ACRP, by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays. Their influence on cell cycle and apoptosis were analyzed by using flow cytometry. Murici and tapereba cell extracts exhibited a strong bioactivity by inhibiting A2780 and ACRP cell viability by 76.37% and 78.37%, respectively, besides modulating the cell cycle and inducing apoptotic cell death. Our results open new perspectives for the development of innovative therapeutic strategies using these Amazon fruit extracts to sensitize ovarian cancer cells to current chemotherapeutic options.
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Keywords:
Byrsonima crassifolia; Byrsonima verbascifolia; Spondias mombin; carotenoids; cisplatin; ovarian cancer
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MDPI and ACS Style
de Souza, V.R.; Brum, M.C.M.; Guimarães, I.S.; dos Santos, P.F.; do Amaral, T.O.; Abreu, J.P.; Passos, T.; Freitas-Silva, O.; Gimba, E.R.P.; Teodoro, A.J. Amazon Fruits Inhibit Growth and Promote Pro-apoptotic Effects on Human Ovarian Carcinoma Cell Lines. Biomolecules 2019, 9, 707.
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