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Open AccessEditor’s ChoiceArticle

Identification and Isolation of Active Compounds from Astragalus membranaceus that Improve Insulin Secretion by Regulating Pancreatic β-Cell Metabolism

1
College of Korean Medicine, Gachon University, Seongnam 13120, Korea
2
Department of Life and Nanopharmaceutical Sciences, Graduate School, Kyung Hee University, Seoul 02447, Korea
*
Authors to whom correspondence should be addressed.
Biomolecules 2019, 9(10), 618; https://doi.org/10.3390/biom9100618
Received: 4 October 2019 / Revised: 16 October 2019 / Accepted: 17 October 2019 / Published: 17 October 2019
In type 2 diabetes (T2D), insufficient secretion of insulin from the pancreatic β-cells contributes to high blood glucose levels, associated with metabolic dysregulation. Interest in natural products to complement or replace existing antidiabetic medications has increased. In this study, we examined the effect of Astragalus membranaceus extract (ASME) and its compounds 19 on glucose-stimulated insulin secretion (GSIS) from pancreatic β-cells. ASME and compounds 19 isolated from A. membranaceus stimulated insulin secretion in INS-1 cells without inducing cytotoxicity. A further experiment showed that compounds 2, 3, and 5 enhanced the phosphorylation of total insulin receptor substrate-2 (IRS-2), phosphatidylinositol 3-kinase (PI3K), and Akt, and activated pancreatic and duodenal homeobox-1 (PDX-1) and peroxisome proliferator-activated receptor-γ (PPAR-γ), which are associated with β-cell function and insulin secretion. The data suggest that two isoflavonoids (2 and 3) and a nucleoside (compound 5), isolated from the roots of A. membranaceus, have the potential to improve insulin secretion in β-cells, representing the first step towards the development of potent antidiabetic drugs. View Full-Text
Keywords: Astragalus membranaceus; insulin; PI3K; AKT; PPARγ; PDX-1 Astragalus membranaceus; insulin; PI3K; AKT; PPARγ; PDX-1
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MDPI and ACS Style

Lee, D.; Lee, D.H.; Choi, S.; Lee, J.S.; Jang, D.S.; Kang, K.S. Identification and Isolation of Active Compounds from Astragalus membranaceus that Improve Insulin Secretion by Regulating Pancreatic β-Cell Metabolism. Biomolecules 2019, 9, 618.

AMA Style

Lee D, Lee DH, Choi S, Lee JS, Jang DS, Kang KS. Identification and Isolation of Active Compounds from Astragalus membranaceus that Improve Insulin Secretion by Regulating Pancreatic β-Cell Metabolism. Biomolecules. 2019; 9(10):618.

Chicago/Turabian Style

Lee, Dahae; Lee, Da H.; Choi, Sungyoul; Lee, Jin S.; Jang, Dae S.; Kang, Ki S. 2019. "Identification and Isolation of Active Compounds from Astragalus membranaceus that Improve Insulin Secretion by Regulating Pancreatic β-Cell Metabolism" Biomolecules 9, no. 10: 618.

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