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Biomolecules 2019, 9(1), 4; https://doi.org/10.3390/biom9010004

Nitric Oxide Release from Antimicrobial Peptide Hydrogels for Wound Healing

1,2
,
1,3,4,5,* , 1,2,3
,
6
,
1,3,7
and
1,2,3,*
1
i3S—Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal
2
IBMC—Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal
3
ICBAS—Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
4
Laboratório de Farmacologia, Departamento de Ciências do Medicamento, Faculdade de Farmácia, Universidade do Porto, Viterbo Ferreira 228, 4050-313 Porto, Portugal
5
IPATIMUP—Instituto de Patologia e Imunologia Molecular, Universidade do Porto, Rua Júlio Amaral de Carvalho, 45, 4200-135 Porto, Portugal
6
LAQV/REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal
7
INEB—Instituto de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal
*
Authors to whom correspondence should be addressed.
Received: 20 November 2018 / Revised: 14 December 2018 / Accepted: 19 December 2018 / Published: 21 December 2018
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Abstract

Nitric oxide (NO) is an endogenously produced molecule that has been implicated in several wound healing mechanisms. Its topical delivery may improve healing in acute or chronic wounds. In this study an antimicrobial peptide was synthesized which self-assembled upon a pH shift, forming a hydrogel. The peptide was chemically functionalized to incorporate a NO-donor moiety on lysine residues. The extent of the reaction was measured by ninhydrin assay and the NO release rate was quantified via the Griess reaction method. The resulting compound was evaluated for its antimicrobial activity against Escherichia coli, and its effect on collagen production by fibroblasts was assessed. Time-kill curves point to an initial increase in bactericidal activity of the functionalized peptide, and collagen production by human dermal fibroblasts when incubated with the NO-functionalized peptide showed a dose-dependent increase in the presence of the NO donor within a range of 0–20 μM. View Full-Text
Keywords: Fmoc-Pexiganan; NO release; antimicrobial activity; collagen Fmoc-Pexiganan; NO release; antimicrobial activity; collagen
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MDPI and ACS Style

Durão, J.; Vale, N.; Gomes, S.; Gomes, P.; Barrias, C.C.; Gales, L. Nitric Oxide Release from Antimicrobial Peptide Hydrogels for Wound Healing. Biomolecules 2019, 9, 4.

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