Next Article in Journal
Searching for Chymase Inhibitors among Chamomile Compounds Using a Computational-Based Approach
Previous Article in Journal
Cyclodextrins in Parkinson’s Disease
Article Menu

Export Article

Open AccessArticle
Biomolecules 2019, 9(1), 4;

Nitric Oxide Release from Antimicrobial Peptide Hydrogels for Wound Healing

1,3,4,5,* , 1,2,3
i3S—Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal
IBMC—Instituto de Biologia Molecular e Celular, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal
ICBAS—Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
Laboratório de Farmacologia, Departamento de Ciências do Medicamento, Faculdade de Farmácia, Universidade do Porto, Viterbo Ferreira 228, 4050-313 Porto, Portugal
IPATIMUP—Instituto de Patologia e Imunologia Molecular, Universidade do Porto, Rua Júlio Amaral de Carvalho, 45, 4200-135 Porto, Portugal
LAQV/REQUIMTE, Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade do Porto, Rua do Campo Alegre, 687, 4169-007 Porto, Portugal
INEB—Instituto de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal
Authors to whom correspondence should be addressed.
Received: 20 November 2018 / Revised: 14 December 2018 / Accepted: 19 December 2018 / Published: 21 December 2018
Full-Text   |   PDF [1162 KB, uploaded 25 December 2018]   |  
  |   Review Reports


Nitric oxide (NO) is an endogenously produced molecule that has been implicated in several wound healing mechanisms. Its topical delivery may improve healing in acute or chronic wounds. In this study an antimicrobial peptide was synthesized which self-assembled upon a pH shift, forming a hydrogel. The peptide was chemically functionalized to incorporate a NO-donor moiety on lysine residues. The extent of the reaction was measured by ninhydrin assay and the NO release rate was quantified via the Griess reaction method. The resulting compound was evaluated for its antimicrobial activity against Escherichia coli, and its effect on collagen production by fibroblasts was assessed. Time-kill curves point to an initial increase in bactericidal activity of the functionalized peptide, and collagen production by human dermal fibroblasts when incubated with the NO-functionalized peptide showed a dose-dependent increase in the presence of the NO donor within a range of 0–20 μM. View Full-Text
Keywords: Fmoc-Pexiganan; NO release; antimicrobial activity; collagen Fmoc-Pexiganan; NO release; antimicrobial activity; collagen

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Durão, J.; Vale, N.; Gomes, S.; Gomes, P.; Barrias, C.C.; Gales, L. Nitric Oxide Release from Antimicrobial Peptide Hydrogels for Wound Healing. Biomolecules 2019, 9, 4.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Biomolecules EISSN 2218-273X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top