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Open AccessArticle

Giantin Is Required for Post-Alcohol Recovery of Golgi in Liver Cells

1
Department of Internal Medicine and VA-Nebraska-Western Iowa Health Care System, Omaha, NE 68105, USA
2
Department of Biochemistry and Molecular Biology, College of Medicine, University of Nebraska Medical Center, Omaha, NE 68198, USA
3
The Nebraska Center for Integrated Biomolecular Communication, Lincoln, NE 68588, USA
4
The Fred and Pamela Buffett Cancer Center, Omaha, NE 68106, USA
*
Author to whom correspondence should be addressed.
Biomolecules 2018, 8(4), 150; https://doi.org/10.3390/biom8040150
Received: 22 October 2018 / Revised: 9 November 2018 / Accepted: 12 November 2018 / Published: 16 November 2018
(This article belongs to the Collection Multi-Organ Alcohol-Related Damage: Mechanisms and Treatment)
In hepatocytes and alcohol-metabolizing cultured cells, Golgi undergoes ethanol (EtOH)-induced disorganization. Perinuclear and organized Golgi is important in liver homeostasis, but how the Golgi remains intact is unknown. Work from our laboratories showed that EtOH-altered cellular function could be reversed after alcohol removal; we wanted to determine whether this recovery would apply to Golgi. We used alcohol-metabolizing HepG2 (VA-13) cells (cultured with or without EtOH for 72 h) and rat hepatocytes (control and EtOH-fed (Lieber–DeCarli diet)). For recovery, EtOH was removed and replenished with control medium (48 h for VA-13 cells) or control diet (10 days for rats). Results: EtOH-induced Golgi disassembly was associated with de-dimerization of the largest Golgi matrix protein giantin, along with impaired transport of selected hepatic proteins. After recovery from EtOH, Golgi regained their compact structure, and alterations in giantin and protein transport were restored. In VA-13 cells, when we knocked down giantin, Rab6a GTPase or non-muscle myosin IIB, minimal changes were observed in control conditions, but post-EtOH recovery was impaired. Conclusions: These data provide a link between Golgi organization and plasma membrane protein expression and identify several proteins whose expression is important to maintain Golgi structure during the recovery phase after EtOH administration. View Full-Text
Keywords: alcohol-induced Golgi disorganization; Golgi recovery; giantin; hepatic proteins; ethanol withdrawal alcohol-induced Golgi disorganization; Golgi recovery; giantin; hepatic proteins; ethanol withdrawal
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MDPI and ACS Style

Casey, C.A.; Thomes, P.; Manca, S.; Petrosyan, A. Giantin Is Required for Post-Alcohol Recovery of Golgi in Liver Cells. Biomolecules 2018, 8, 150.

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