A Marine Diterpenoid Modulates the Proteasome Activity in Murine Macrophages Stimulated with LPS
Abstract
:1. Introduction
2. Materials and Methods
2.1. Mice
2.2. Acute Pulmonary Inflammation
2.3. Cell Culture and Proteasome Activity Assay
2.4. Western Blot Analysis
2.5. Major Histocompatibility Complex Class I Flow Cytometry Analysis
2.6. Quantitative Real Time Polymerase Chain Reaction
2.7. Purified Proteasome Activity Assay
2.8. Cytokine Measurements
2.9. Molecular Docking Simulation
2.10. Statistical Analysis
3. Results
3.1. Compound 1 Inhibits the Activity of the Proteasome with the Consequent Accumulation of Ubiquitinated Proteins
3.2. Compound 1 did not Affect the Expression of β1i and β5i Subunits in Peritoneal Macrophages Stimulated with Lypopolisaccharide
3.3. Compound 1 Inhibits the Chemotrypsin-like Activity of Purified Proteasomes
3.4. Compound 1 Inhibits the Expression of MHC-I In Vitro and the Production of Pro-Inflammatory Mediators In Vivo
3.5. Compound 1 Appears to Bind to the Catalytic Pocket of β5i Subunit of the Immunoproteasome
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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González, Y.; Doens, D.; Cruz, H.; Santamaría, R.; Gutiérrez, M.; Llanes, A.; Fernández, P.L. A Marine Diterpenoid Modulates the Proteasome Activity in Murine Macrophages Stimulated with LPS. Biomolecules 2018, 8, 109. https://doi.org/10.3390/biom8040109
González Y, Doens D, Cruz H, Santamaría R, Gutiérrez M, Llanes A, Fernández PL. A Marine Diterpenoid Modulates the Proteasome Activity in Murine Macrophages Stimulated with LPS. Biomolecules. 2018; 8(4):109. https://doi.org/10.3390/biom8040109
Chicago/Turabian StyleGonzález, Yisett, Deborah Doens, Héctor Cruz, Ricardo Santamaría, Marcelino Gutiérrez, Alejandro Llanes, and Patricia L. Fernández. 2018. "A Marine Diterpenoid Modulates the Proteasome Activity in Murine Macrophages Stimulated with LPS" Biomolecules 8, no. 4: 109. https://doi.org/10.3390/biom8040109