Amyloid Assembly Endows Gad m 1 with Biomineralization Properties
1
IMDEA Nanoscience, 28049 Madrid, Spain
2
Departamento de Química Inorgánica, Facultad de Químicas, Universidad Complutense, 28040 Madrid, Spain
3
Instituto de Investigación Hospital Universitario La Paz (IdiPaz), 28046 Madrid, Spain
4
Instituto Química-Física “Rocasolano”, Consejo Superior de Investigaciones Científicas, 28006 Madrid, Spain
*
Author to whom correspondence should be addressed.
Biomolecules 2018, 8(1), 13; https://doi.org/10.3390/biom8010013
Received: 18 January 2018
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Revised: 2 March 2018
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Accepted: 16 March 2018
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Published: 20 March 2018
(This article belongs to the Special Issue Functional Amyloids)
Acid proteins capable of nucleating Ca2+ and displaying aggregation capacity play key roles in the formation of calcium carbonate biominerals. The helix-loop helix EF-hands are the most common Ca2+-binding motifs in proteins. Calcium is bound by the loop region. These motifs are found in many proteins that are regulated by calcium. Gad m 1, an Atlantic cod β-parvalbumin isoform, is a monomeric EF-hand protein that acts as a Ca2+ buffer in fish muscle; the neutral and acid apo-forms of this protein can form amyloids. Since Ca2+-nucleating proteins have a propensity to form extended β-strand structures, we wondered whether amyloid assemblies of an EF-hand protein were able to influence calcium carbonate crystallization in vitro. Here, we used the Gad m 1 chain as a model to generate monomeric and amyloid assemblies and to analyze their effect on calcite formation in vitro. We found that only amyloid assemblies alter calcite morphology.
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Keywords:
amyloids; Gad m 1; EF-hand motif; calcium carbonate precipitation; calcite
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MDPI and ACS Style
Castellanos, M.; Torres-Pardo, A.; Rodríguez-Pérez, R.; Gasset, M. Amyloid Assembly Endows Gad m 1 with Biomineralization Properties. Biomolecules 2018, 8, 13. https://doi.org/10.3390/biom8010013
AMA Style
Castellanos M, Torres-Pardo A, Rodríguez-Pérez R, Gasset M. Amyloid Assembly Endows Gad m 1 with Biomineralization Properties. Biomolecules. 2018; 8(1):13. https://doi.org/10.3390/biom8010013
Chicago/Turabian StyleCastellanos, Milagros, Almudena Torres-Pardo, Rosa Rodríguez-Pérez, and María Gasset. 2018. "Amyloid Assembly Endows Gad m 1 with Biomineralization Properties" Biomolecules 8, no. 1: 13. https://doi.org/10.3390/biom8010013
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