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m1A Post‐Transcriptional Modification in tRNAs
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Mapping Post‐Transcriptional Modifications onto Transfer Ribonucleic Acid Sequences by Liquid Chromatography Tandem Mass Spectrometry

Rieveschl Laboratories for Mass Spectrometry, Department of Chemistry, University of Cincinnati, P.O. Box 210172, Cincinnati, OH 45221‐0172, USA
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Academic Editor: Jürg Bähler
Biomolecules 2017, 7(1), 21; https://doi.org/10.3390/biom7010021
Received: 30 December 2016 / Accepted: 15 February 2017 / Published: 22 February 2017
(This article belongs to the Collection RNA Modifications)
Liquid chromatography, coupled with tandem mass spectrometry, has become one of the most popular methods for the analysis of post‐transcriptionally modified transfer ribonucleic acids (tRNAs). Given that the information collected using this platform is entirely determined by the mass of the analyte, it has proven to be the gold standard for accurately assigning nucleobases to the sequence. For the past few decades many labs have worked to improve the analysis, contiguous to instrumentation manufacturers developing faster and more sensitive instruments. With biological discoveries relating to ribonucleic acid happening more frequently, mass spectrometry has been invaluable in helping to understand what is happening at the molecular level. Here we present a brief overview of the methods that have been developed and refined for the analysis of modified tRNAs by liquid chromatography tandem mass spectrometry. View Full-Text
Keywords: modified nucleosides; RNA sequencing; tRNA; tandem mass spectrometry; LC‐MS/MS modified nucleosides; RNA sequencing; tRNA; tandem mass spectrometry; LC‐MS/MS
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MDPI and ACS Style

Ross, R.L.; Cao, X.; Limbach, P.A. Mapping Post‐Transcriptional Modifications onto Transfer Ribonucleic Acid Sequences by Liquid Chromatography Tandem Mass Spectrometry. Biomolecules 2017, 7, 21. https://doi.org/10.3390/biom7010021

AMA Style

Ross RL, Cao X, Limbach PA. Mapping Post‐Transcriptional Modifications onto Transfer Ribonucleic Acid Sequences by Liquid Chromatography Tandem Mass Spectrometry. Biomolecules. 2017; 7(1):21. https://doi.org/10.3390/biom7010021

Chicago/Turabian Style

Ross, Robert L., Xiaoyu Cao, and Patrick A. Limbach. 2017. "Mapping Post‐Transcriptional Modifications onto Transfer Ribonucleic Acid Sequences by Liquid Chromatography Tandem Mass Spectrometry" Biomolecules 7, no. 1: 21. https://doi.org/10.3390/biom7010021

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