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Biomolecules 2015, 5(1), 3-19;

Biodegradable Chitosan Nanoparticle Coatings on Titanium for the Delivery of BMP-2

Institut für Technische Chemie, Technische Universität Braunschweig, Hans-Sommer-Str. 10, 38106 Braunschweig, Germany
Helmholtz-Zentrum für Infektionsforschung, Signaling and Gene Regulation, 38124 Braunschweig, Germany
Author to whom correspondence should be addressed.
Academic Editor: Jürg Bähler
Received: 25 August 2014 / Accepted: 31 December 2014 / Published: 8 January 2015
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A simple method for the functionalization of a common implant material (Ti6Al4V) with biodegradable, drug loaded chitosan-tripolyphosphate (CS-TPP) nanoparticles is developed in order to enhance the osseointegration of endoprostheses after revision operations. The chitosan used has a tailored degree of acetylation which allows for a fast biodegradation by lysozyme. The degradability of chitosan is proven via viscometry. Characteristics and degradation of nanoparticles formed with TPP are analyzed using dynamic light scattering. The particle degradation via lysozyme displays a decrease in particle diameter of 40% after 4 days. Drug loading and release is investigated for the nanoparticles with bone morphogenetic protein 2 (BMP-2), using ELISA and the BRE luciferase test for quantification and bioactivity evaluation. Furthermore, nanoparticle coatings on titanium substrates are created via spray-coating and analyzed by ellipsometry, scanning electron microscopy and X-ray photoelectron spectroscopy. Drug loaded nanoparticle coatings with biologically active BMP-2 are obtained in vitro within this work. Additionally, an in vivo study in mice indicates the dose dependent induction of ectopic bone growth through CS-TPP-BMP-2 nanoparticles. These results show that biodegradable CS-TPP coatings can be utilized to present biologically active BMP-2 on common implant materials like Ti6Al4V. View Full-Text
Keywords: BMP-2 delivery; nanoparticle degradation; coatings; chitosan; biodegradable BMP-2 delivery; nanoparticle degradation; coatings; chitosan; biodegradable

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Poth, N.; Seiffart, V.; Gross, G.; Menzel, H.; Dempwolf, W. Biodegradable Chitosan Nanoparticle Coatings on Titanium for the Delivery of BMP-2. Biomolecules 2015, 5, 3-19.

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