Dynamic Interplay Between miR-124-3p and EGF in the Regulation of Overgrowth via RNA Signaling
Abstract
1. Introduction
2. Material Methods
2.1. Animals
2.2. RNA Microinjection in Fertilised Eggs
2.3. Three-Dimensional Hippocampal Cell Culture
2.4. RNA Extraction, RT-PCR, Pre-Amplification, and qPCR
2.5. miRNA Expression by qPCR
2.6. Behavioural Tests
2.7. Statistical Analyses
3. Results
3.1. Recapitulation of the “Giant” Phenotype in the Balb/C Background Following Microinjection of miR-124-3p RNA into Fertilised Mouse Eggs
3.2. Behavioural Findings
3.3. miR-124-3p Microinjection Does Not Significantly Alter miR-124 Expression Levels in Early Embryos or Adult Hippocampus
3.4. Neuronal Differentiation Could Be Promoted in miR-124-3p* Embryos During the Early Stage
3.5. The Role of miR-124-3p* in Regulating Body Growth Pathway During Early Embryonic Stage
3.6. miR-124-3p* Embryos Are Sensitive to Growth Factors Such as EGF During the Late Embryonic Period
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Korkmaz Bayram, K.; Bayram, A.; Yilmaz Sukranli, Z.; Mehmetbeyoglu Duman, E.; Aybuga, F.; Tufan Benli, E.; Taheri, S.; Ozkul, Y.; Rassoulzadegan, M. Dynamic Interplay Between miR-124-3p and EGF in the Regulation of Overgrowth via RNA Signaling. Biomolecules 2025, 15, 1186. https://doi.org/10.3390/biom15081186
Korkmaz Bayram K, Bayram A, Yilmaz Sukranli Z, Mehmetbeyoglu Duman E, Aybuga F, Tufan Benli E, Taheri S, Ozkul Y, Rassoulzadegan M. Dynamic Interplay Between miR-124-3p and EGF in the Regulation of Overgrowth via RNA Signaling. Biomolecules. 2025; 15(8):1186. https://doi.org/10.3390/biom15081186
Chicago/Turabian StyleKorkmaz Bayram, Keziban, Arslan Bayram, Zeynep Yilmaz Sukranli, Ecmel Mehmetbeyoglu Duman, Fatma Aybuga, Esra Tufan Benli, Serpil Taheri, Yusuf Ozkul, and Minoo Rassoulzadegan. 2025. "Dynamic Interplay Between miR-124-3p and EGF in the Regulation of Overgrowth via RNA Signaling" Biomolecules 15, no. 8: 1186. https://doi.org/10.3390/biom15081186
APA StyleKorkmaz Bayram, K., Bayram, A., Yilmaz Sukranli, Z., Mehmetbeyoglu Duman, E., Aybuga, F., Tufan Benli, E., Taheri, S., Ozkul, Y., & Rassoulzadegan, M. (2025). Dynamic Interplay Between miR-124-3p and EGF in the Regulation of Overgrowth via RNA Signaling. Biomolecules, 15(8), 1186. https://doi.org/10.3390/biom15081186