Next Article in Journal
Advances in the Pathogenesis of Steroid-Associated Osteonecrosis of the Femoral Head
Previous Article in Journal
Effect of Acute Nutritional Ketosis on Circulating Levels of Growth Differentiation Factor 15: Findings from a Cross-Over Randomised Controlled Trial
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biomolecules
Volume 14
Issue 6
10.3390/biom14060666
biomolecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Combination of Hydrolysable Tannins and Zinc Oxide on Enterocyte Functionality: In Vitro Insights

by
Francesca Ciaramellano
1,2,*,
Lucia Scipioni
2,3,
Benedetta Belà
1,
Giulia Pignataro
1,
Giacomo Giacovazzo
1,
Clotilde Beatrice Angelucci
1,
Roberto Giacominelli-Stuffler
1,
Alessandro Gramenzi
1,† and
Sergio Oddi
1,2,*,†
1
Department of Veterinary Medicine, University of Teramo, 64100 Teramo, Italy
2
European Center for Brain Research (CERC), Santa Lucia Foundation IRCCS, 00143 Rome, Italy
3
Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, Via Vetoio Snc, 67100 L’Aquila, Italy
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Biomolecules 2024, 14(6), 666; https://doi.org/10.3390/biom14060666
Submission received: 27 April 2024 / Revised: 24 May 2024 / Accepted: 3 June 2024 / Published: 6 June 2024
Browse Figures
Versions Notes

Abstract

The management of gastrointestinal disease in animals represents a significant challenge in veterinary and zootechnic practice. Traditionally, acute symptoms have been treated with antibiotics and high doses of zinc oxide (ZnO). However, concerns have been raised regarding the potential for microbial resistance and ecological detriment due to the excessive application of this compound. These concerns highlight the urgency of minimizing the use of ZnO and exploring sustainable nutritional solutions. Hydrolysable tannins (HTs), which are known for their role in traditional medicine for acute gastrointestinal issues, have emerged as a promising alternative. This study examined the combined effect of food-grade HTs and subtherapeutic ZnO concentration on relevant biological functions of Caco-2 cells, a widely used model of the intestinal epithelial barrier. We found that, when used together, ZnO and HTs (ZnO/HTs) enhanced tissue repair and improved epithelial barrier function, normalizing the expression and functional organization of tight junction proteins. Finally, the ZnO/HTs combination strengthened enterocytes’ defense against oxidative stress induced by inflammation stimuli. In conclusion, combining ZnO and HTs may offer a suitable and practical approach for decreasing ZnO levels in veterinary nutritional applications.
Keywords: Caco-2 cells; epithelial barrier function; gastrointestinal diseases; zinc oxide (ZnO); hydrolysable tannins (HTs); epithelial barrier function; oxidative stress Caco-2 cells; epithelial barrier function; gastrointestinal diseases; zinc oxide (ZnO); hydrolysable tannins (HTs); epithelial barrier function; oxidative stress

Share and Cite

MDPI and ACS Style

Ciaramellano, F.; Scipioni, L.; Belà, B.; Pignataro, G.; Giacovazzo, G.; Angelucci, C.B.; Giacominelli-Stuffler, R.; Gramenzi, A.; Oddi, S. Combination of Hydrolysable Tannins and Zinc Oxide on Enterocyte Functionality: In Vitro Insights. Biomolecules 2024, 14, 666. https://doi.org/10.3390/biom14060666

AMA Style

Ciaramellano F, Scipioni L, Belà B, Pignataro G, Giacovazzo G, Angelucci CB, Giacominelli-Stuffler R, Gramenzi A, Oddi S. Combination of Hydrolysable Tannins and Zinc Oxide on Enterocyte Functionality: In Vitro Insights. Biomolecules. 2024; 14(6):666. https://doi.org/10.3390/biom14060666

Chicago/Turabian Style

Ciaramellano, Francesca, Lucia Scipioni, Benedetta Belà, Giulia Pignataro, Giacomo Giacovazzo, Clotilde Beatrice Angelucci, Roberto Giacominelli-Stuffler, Alessandro Gramenzi, and Sergio Oddi. 2024. "Combination of Hydrolysable Tannins and Zinc Oxide on Enterocyte Functionality: In Vitro Insights" Biomolecules 14, no. 6: 666. https://doi.org/10.3390/biom14060666

APA Style

Ciaramellano, F., Scipioni, L., Belà, B., Pignataro, G., Giacovazzo, G., Angelucci, C. B., Giacominelli-Stuffler, R., Gramenzi, A., & Oddi, S. (2024). Combination of Hydrolysable Tannins and Zinc Oxide on Enterocyte Functionality: In Vitro Insights. Biomolecules, 14(6), 666. https://doi.org/10.3390/biom14060666

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop