Circulating H3K27 Methylated Nucleosome Plasma Concentration: Synergistic Information with Circulating Tumor DNA Molecular Profiling
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Population
2.2. CfDNA Collection
2.3. Library Preparation for DNA Sequencing
2.4. Nu.Q® ImmunoAssays
2.5. Tissue Microarray (TMA)
2.6. Statistics
2.7. Study Limitations
3. Results
3.1. High Levels of Circulating H3K27Me3 Nucleosomes Are Observed in NSCLC Samples at Diagnosis
3.2. H3K27Me3-Nucleosome Levels Correlate with cfDNA Quantity and Percentage of Mutational Allele Fraction (MAF) at Diagnosis
3.3. H3K27Me3 Expression Level in Normal and Adenocarcinoma Tissue
3.4. Relevant Combined Information from the Circulating H3K27Me3-Nucleosome Levels and ctDNA Molecular Profile at Diagnosis
3.5. High Level of Circulating H3K27Me3 Nucleosomes Observed in NSCLC Samples during Treatment Is More Pronounced in the Presence of Mutated ctDNA
3.6. Additive Value of Both Biomarkers: H3K27Me3 Nucleosomes and ctDNA
3.7. High Circulating H3K27Me3-Nucleosome Levels Are Preferentially Associated with TP53 Mutations during Treatment
4. Discussion
5. Conclusions
6. Patents
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Grolleau, E.; Candiracci, J.; Lescuyer, G.; Barthelemy, D.; Benzerdjeb, N.; Haon, C.; Geiguer, F.; Raffin, M.; Hardat, N.; Balandier, J.; et al. Circulating H3K27 Methylated Nucleosome Plasma Concentration: Synergistic Information with Circulating Tumor DNA Molecular Profiling. Biomolecules 2023, 13, 1255. https://doi.org/10.3390/biom13081255
Grolleau E, Candiracci J, Lescuyer G, Barthelemy D, Benzerdjeb N, Haon C, Geiguer F, Raffin M, Hardat N, Balandier J, et al. Circulating H3K27 Methylated Nucleosome Plasma Concentration: Synergistic Information with Circulating Tumor DNA Molecular Profiling. Biomolecules. 2023; 13(8):1255. https://doi.org/10.3390/biom13081255
Chicago/Turabian StyleGrolleau, Emmanuel, Julie Candiracci, Gaelle Lescuyer, David Barthelemy, Nazim Benzerdjeb, Christine Haon, Florence Geiguer, Margaux Raffin, Nathalie Hardat, Julie Balandier, and et al. 2023. "Circulating H3K27 Methylated Nucleosome Plasma Concentration: Synergistic Information with Circulating Tumor DNA Molecular Profiling" Biomolecules 13, no. 8: 1255. https://doi.org/10.3390/biom13081255