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Article

Head-to-Head Comparison of Different Blood Collecting Tubes for Quantification of Alzheimer’s Disease Biomarkers in Plasma

by
Lijun Jiang
1,†,
Xulong Ding
1,2,†,
Wenxiao Wang
3,
Xiaobin Yang
3,
Tao Li
1,4,5,* and
Peng Lei
1,*
1
Mental Health Center and Department of Neurology, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu 610041, China
2
Dushu Lake Hospital Affiliated to Soochow University, Medical Center of Soochow University, Suzhou Dushu Lake Hospital, Suzhou 215125, China
3
Deyang Mental Health Center, Deyang 618099, China
4
Department of Neurobiology, Affiliated Mental Health Center & Hangzhou Seventh People’s Hospital, Zhejiang University School of Medicine, Hangzhou 310063, China
5
NHC and CAMS Key Laboratory of Medical Neurobiology, MOE Frontier Science Center for Brain Science and Brain-machine Integration, School of Brain Science and Brain Medicine, Zhejiang University, Hangzhou 310012, China
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Biomolecules 2022, 12(9), 1194; https://doi.org/10.3390/biom12091194
Submission received: 16 July 2022 / Revised: 24 August 2022 / Accepted: 26 August 2022 / Published: 28 August 2022
(This article belongs to the Special Issue New Advances in the Molecular Mechanism and Target Therapy in Alzheimer’s Diseases)
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Abstract

To examine whether the type of blood collection tubes affects the quantification of plasma biomarkers for Alzheimer’s disease analyzed with a single-molecule array (Simoa), we recruited a healthy cohort (n = 34, 11 males, mean age = 28.7 ± 7.55) and collected plasma in the following tubes: dipotassium ethylenediaminetetraacetic acid (K2-EDTA), heparin lithium (Li-Hep), and heparin sodium (Na-Hep). Plasma tau, phosphorylated tau 181 (p-tau181), amyloid β (1–40) (Aβ40), and amyloid β (1–42) (Aβ42) were quantified using Simoa. We compared the value of plasma analytes, as well as the effects of sex on the measurements. We found that plasma collected in Li-Hep and Na-Hep tubes yielded significantly higher tau and p-tau181 levels compared to plasma collected in K2-EDTA tubes from the same person, but there was no difference in the measured values of the Aβ40, Aβ42, and Aβ42/40 ratio. Therefore, the type of blood collecting tubes should be considered when planning studies that measure plasma tau.
Keywords: blood collection tubes; tau; Alzheimer’s disease; Simoa; EDTA; heparin blood collection tubes; tau; Alzheimer’s disease; Simoa; EDTA; heparin

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MDPI and ACS Style

Jiang, L.; Ding, X.; Wang, W.; Yang, X.; Li, T.; Lei, P. Head-to-Head Comparison of Different Blood Collecting Tubes for Quantification of Alzheimer’s Disease Biomarkers in Plasma. Biomolecules 2022, 12, 1194. https://doi.org/10.3390/biom12091194

AMA Style

Jiang L, Ding X, Wang W, Yang X, Li T, Lei P. Head-to-Head Comparison of Different Blood Collecting Tubes for Quantification of Alzheimer’s Disease Biomarkers in Plasma. Biomolecules. 2022; 12(9):1194. https://doi.org/10.3390/biom12091194

Chicago/Turabian Style

Jiang, Lijun, Xulong Ding, Wenxiao Wang, Xiaobin Yang, Tao Li, and Peng Lei. 2022. "Head-to-Head Comparison of Different Blood Collecting Tubes for Quantification of Alzheimer’s Disease Biomarkers in Plasma" Biomolecules 12, no. 9: 1194. https://doi.org/10.3390/biom12091194

APA Style

Jiang, L., Ding, X., Wang, W., Yang, X., Li, T., & Lei, P. (2022). Head-to-Head Comparison of Different Blood Collecting Tubes for Quantification of Alzheimer’s Disease Biomarkers in Plasma. Biomolecules, 12(9), 1194. https://doi.org/10.3390/biom12091194

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