Transcriptomic Profiling Reveals That HMGB1 Induces Macrophage Polarization Different from Classical M1
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Harvesting and Culturing of BMDMs
2.3. Recombinant HMGB1 Production
2.4. Cell Culture Experiments
2.5. RNA-Seq
2.6. Verification of Differentially Expressed Genes Identified in RNA-Seq
2.7. Oil Red O Staining
2.8. Statistical Analysis
3. Results
3.1. dsHMGB1-, LPS- and LPS/IFNγ-Stimulation Resulted in Similar Pro-Inflammatory Gene Expression Patterns, While frHMGB1 Overlapped with PBS Control
3.2. Identification of Gene Signatures in dsHMGB1- Compared to LPS/IFNγ-Treated BMDMs
3.3. Identification of Gene Signatures in dsHMGB1- Compared to LPS-Treated BMDMs
3.4. frHMGB1 and dsHMGB1 Attenuated BMDM-Derived Foam Cell Formation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Qu, H.; Heinbäck, R.; Salo, H.; Ewing, E.; Espinosa, A.; Aulin, C.; Erlandsson Harris, H. Transcriptomic Profiling Reveals That HMGB1 Induces Macrophage Polarization Different from Classical M1. Biomolecules 2022, 12, 779. https://doi.org/10.3390/biom12060779
Qu H, Heinbäck R, Salo H, Ewing E, Espinosa A, Aulin C, Erlandsson Harris H. Transcriptomic Profiling Reveals That HMGB1 Induces Macrophage Polarization Different from Classical M1. Biomolecules. 2022; 12(6):779. https://doi.org/10.3390/biom12060779
Chicago/Turabian StyleQu, Heshuang, Rebecka Heinbäck, Henna Salo, Ewoud Ewing, Alexander Espinosa, Cecilia Aulin, and Helena Erlandsson Harris. 2022. "Transcriptomic Profiling Reveals That HMGB1 Induces Macrophage Polarization Different from Classical M1" Biomolecules 12, no. 6: 779. https://doi.org/10.3390/biom12060779