A Novel and Validated 8-Pyroptosis-Related Genes Based Risk Prediction Model for Diffuse Large B Cell Lymphoma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Data Acquisition
2.2. Pathway Enrichment Analysis
2.3. Establishment and Validation of the Risk Prediction Model
2.4. Construction of Nomogram
2.5. Immune Components Prediction
2.6. Prediction of Chemosensitivity
2.7. Cell Culture
2.8. RT-qPCR
2.9. Statistical Analysis
3. Results
3.1. Identification of 24 Prognostic Pyroptosis Related Genes (PRGs) in DLBCL
3.2. Establishment of a PRGs Oriented Risk Model via LASSO Regression
3.3. Risk Model Performed Sensitively and Robust in Internal and External Validation Datasets
3.4. RT-qPCR and IHC Slice Image Confirmed the Expression of 8 PRGs in Real World
3.5. The Risk Score Predicted the Advance of DLBCL
3.6. The Risk Model Predicted the Sensitivity of Novel Chemotherapy
3.7. The Risk Model Revealed the Remodeling of Immune Landscape in DLBCL
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Genes | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
---|---|---|
HTRA1 | TCCCAACAGTTTGCGCCATAA | CCGGCACCTCTCGTTTAGAAA |
RBBP7 | GAGGAGCGTGTCATCAATGAA | GCATGGGTCATAACCAGGTCATA |
NFE2L2 | TTCCCGGTCACATCGAGAG | TCCTGTTGCATACCGTCTAAATC |
SCAF11 | TGAAAGCAAAGTGTACCAACCT | GGCTCTCTATAAGCTCCTCTGT |
ABL1 | TGAAAAGCTCCGGGTCTTAGG | TTGACTGGCGTGATGTAGTTG |
PAK2 | CACCCGCAGTAGTGACAGAG | GGGTCAATTACAGACCGTGTG |
CPTP | TCTGCGCCGACTCCTACAA | CTCGCCTAGCATCTGCACG |
ADORA3 | GGCCAATGTTACCTACATCACC | CCAGGGCTAGAGAGACAATGAA |
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Ma, J.; Wang, W.; Ma, J.; Xu, Z. A Novel and Validated 8-Pyroptosis-Related Genes Based Risk Prediction Model for Diffuse Large B Cell Lymphoma. Biomolecules 2022, 12, 1835. https://doi.org/10.3390/biom12121835
Ma J, Wang W, Ma J, Xu Z. A Novel and Validated 8-Pyroptosis-Related Genes Based Risk Prediction Model for Diffuse Large B Cell Lymphoma. Biomolecules. 2022; 12(12):1835. https://doi.org/10.3390/biom12121835
Chicago/Turabian StyleMa, Junrui, Wenhan Wang, Jiao Ma, and Zizhen Xu. 2022. "A Novel and Validated 8-Pyroptosis-Related Genes Based Risk Prediction Model for Diffuse Large B Cell Lymphoma" Biomolecules 12, no. 12: 1835. https://doi.org/10.3390/biom12121835