Evaluation of Biointegration and Inflammatory Response to Blood Vessels Produced by Tissue Engineering—Experimental Model in Rabbits
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Handling Conditions and Tissue Acquisition
2.2. Scaffolds Production
2.3. Obtaining Adipose-Derived Stem Cells (ASCs)
2.4. Experimental Groups
2.5. Biological Scaffold and ASCs Seeding
2.6. Surgical Experimentation
2.7. Analysis of Interleukins and Oxidative Stress
2.8. Histomorphology
2.9. Statistical Analysis
3. Results
3.1. Animals
3.2. Vein Decellularization, ASC Characterization, and In Vivo Experimentation
3.3. Interleukin Analysis
3.3.1. Plasma
3.3.2. Frozen Explant Tissue Homogenate
3.3.3. Oxidative Stress by Plasma MDA Analysis
3.4. Histomorphology
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Secondo, M.T.S.; Rodrigues, L.d.S.; Ramos, L.P.M.; Bovolato, A.L.C.; Rodriguez-Sanchez, D.N.; Sobreira, M.L.; Moraes, M.P.d.T.; Bertanha, M. Evaluation of Biointegration and Inflammatory Response to Blood Vessels Produced by Tissue Engineering—Experimental Model in Rabbits. Biomolecules 2022, 12, 1776. https://doi.org/10.3390/biom12121776
Secondo MTS, Rodrigues LdS, Ramos LPM, Bovolato ALC, Rodriguez-Sanchez DN, Sobreira ML, Moraes MPdT, Bertanha M. Evaluation of Biointegration and Inflammatory Response to Blood Vessels Produced by Tissue Engineering—Experimental Model in Rabbits. Biomolecules. 2022; 12(12):1776. https://doi.org/10.3390/biom12121776
Chicago/Turabian StyleSecondo, Mariana Thaís Silva, Lenize da Silva Rodrigues, Leandro Pereira Miranda Ramos, Ana Lívia Carvalho Bovolato, Diego Noé Rodriguez-Sanchez, Marcone Lima Sobreira, Marcelo Padovani de Toledo Moraes, and Matheus Bertanha. 2022. "Evaluation of Biointegration and Inflammatory Response to Blood Vessels Produced by Tissue Engineering—Experimental Model in Rabbits" Biomolecules 12, no. 12: 1776. https://doi.org/10.3390/biom12121776