Next Article in Journal
Chelation Combination—A Strategy to Mitigate the Neurotoxicity of Manganese, Iron, and Copper?
Next Article in Special Issue
Secondary Metabolites Diversity of Aspergillus unguis and Their Bioactivities: A Potential Target to Be Explored
Previous Article in Journal
Receptor for Advanced Glycation End Product, Organ Crosstalk, and Pathomechanism Targets for Comprehensive Molecular Therapeutics in Diabetic Ischemic Stroke
Previous Article in Special Issue
Beneficial Effect of Paeonol on Antibiotic-Associated Inflammatory Response in Mice with Diarrhea
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biomolecules
Volume 12
Issue 11
10.3390/biom12111711
biomolecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Biodefensive Based on Piper nigrum Essential Oil for Controlling of Anopheles aquasalis Larvae: Influence of Temperature (35 °C) and Preservatives

by
Ayná Caroline Marcião Vieira
1,2,
Sidney Gomes Azevedo
1,
Ramon Andrade Linhares
3,
Silvia Cássia Brandão Justiniano
3,
Grafe Oliveira Pontes
3,
Alessandra Ramos Lima
4,
Pedro Henrique Campelo
5,
Jaqueline de Araújo Bezerra
6,
Camila da Costa Pinto
6,
Henrique Duarte da Fonseca Filho
7,
Robert Saraiva Matos
8,
Ştefan Ţălu
9,*,
Vanderlei Salvador Bagnato
4,10,
Natalia Mayumi Inada
4 and
Edgar Aparecido Sanches
1,2
1
Laboratory of Nanostructured Polymers (NANOPOL), Federal University of Amazonas (UFAM), Manaus 69067-005, Brazil
2
Graduate Program in Materials Science and Engineering (PPGCEM), Federal University of Amazonas (UFAM), Manaus 69067-005, Brazil
3
Tropical Medicine Foundation Doctor Heitor Vieira Dourado (FMT-HVD), Manaus 69040-000, Brazil
4
São Carlos Institute of Physics (IFSC), University of São Paulo (USP), São Carlos 13563-120, Brazil
5
Department of Food Technology, Federal University of Viçosa (UFV), Viçosa 36570-900, Brazil
6
Federal Institute of Education, Science and Technology of Amazonas (IFAM), Manaus 69020-120, Brazil
7
Laboratory of Nanomaterials Synthesis and Nanoscopy (LSNN), Federal University of Amazonas (UFAM), Manaus 69067-005, Brazil
8
Amazonian Materials Group, Federal University of Amapá (UNIFAP), Macapá 68903-419, Brazil
9
The Directorate of Research, Development and Innovation Management (DMCDI), Technical University of Cluj-Napoca, 15 Constantin Daicoviciu St., 400020 Cluj-Napoca, Romania
10
Hagler Institute for Advanced Studies, Texas A&M University, College Station, TX 77843-3572, USA
*
Author to whom correspondence should be addressed.
Biomolecules 2022, 12(11), 1711; https://doi.org/10.3390/biom12111711
Submission received: 30 September 2022 / Revised: 11 November 2022 / Accepted: 14 November 2022 / Published: 18 November 2022
(This article belongs to the Special Issue Bioactive Natural Compounds against Animal and Human Pathogens)
Browse Figures
Versions Notes

Abstract

Considerable efforts have been spent on the development of biodefensives based on the encapsulation of essential oils for controlling of urban pests from their larval stage, especially as anopheline controlling agents. The larval source management of Anopheles aquasalis is important for malaria prevention. For this reason, this research proposes larvicidal biodefensives based on polymeric particles loaded with Piper nigrum essential oil, considering the influence of temperature (35 °C) and preservatives on the formulation stability. The biodefensive containing the preservative phenoxyethanol/methylisothiazolinone (PNE) resulted in 5 months of shelf-life storage with an Encapsulation Efficiency (EE%) of essential oil of 70%. The biodefensive PNE (containing 500 µg.mL−1 of encapsulated essential oil) presented a polydisperse particle size distribution, ranging from D10 = (127 ± 10) nm to D90 = (472 ± 78) nm and a particle mean size of (236 ± 34) nm. The AFM images revealed a spherical morphology with an external surface almost regular and smooth. The controlled release of the essential oil was evaluated up to 72 h according to the Korsmeyer-Peppas mathematical model, confirming the anomalous transport (n = 0.64 in pH = 3 and pH = 10, and n = 0.65 in pH = 7). The total larvae mortality on the in loco bioassays was almost reached (92%) after 24 h. However, according to the in vitro bioassays applying the in natura essential oil alone, the concentration of 454 μg.mL−1 resulted on the mortality of 70% of the larvae after 24 h. For this reason, the highest efficiency of the biodefensive PNE may be related to the encapsulation of essential oil, delivering the loaded particles more efficiently inside the larvae. From this perspective, the present study shows that a formulation based on P. nigrum essential oil may be taken into account in the integrated management of disease vector mosquitoes.
Keywords: biodefensive; encapsulation; essential oil; Piper nigrum; Anopheles aquasalis biodefensive; encapsulation; essential oil; Piper nigrum; Anopheles aquasalis

Share and Cite

MDPI and ACS Style

Marcião Vieira, A.C.; Azevedo, S.G.; Linhares, R.A.; Brandão Justiniano, S.C.; Pontes, G.O.; Lima, A.R.; Campelo, P.H.; Bezerra, J.d.A.; da Costa Pinto, C.; Fonseca Filho, H.D.d.; et al. Biodefensive Based on Piper nigrum Essential Oil for Controlling of Anopheles aquasalis Larvae: Influence of Temperature (35 °C) and Preservatives. Biomolecules 2022, 12, 1711. https://doi.org/10.3390/biom12111711

AMA Style

Marcião Vieira AC, Azevedo SG, Linhares RA, Brandão Justiniano SC, Pontes GO, Lima AR, Campelo PH, Bezerra JdA, da Costa Pinto C, Fonseca Filho HDd, et al. Biodefensive Based on Piper nigrum Essential Oil for Controlling of Anopheles aquasalis Larvae: Influence of Temperature (35 °C) and Preservatives. Biomolecules. 2022; 12(11):1711. https://doi.org/10.3390/biom12111711

Chicago/Turabian Style

Marcião Vieira, Ayná Caroline, Sidney Gomes Azevedo, Ramon Andrade Linhares, Silvia Cássia Brandão Justiniano, Grafe Oliveira Pontes, Alessandra Ramos Lima, Pedro Henrique Campelo, Jaqueline de Araújo Bezerra, Camila da Costa Pinto, Henrique Duarte da Fonseca Filho, and et al. 2022. "Biodefensive Based on Piper nigrum Essential Oil for Controlling of Anopheles aquasalis Larvae: Influence of Temperature (35 °C) and Preservatives" Biomolecules 12, no. 11: 1711. https://doi.org/10.3390/biom12111711

APA Style

Marcião Vieira, A. C., Azevedo, S. G., Linhares, R. A., Brandão Justiniano, S. C., Pontes, G. O., Lima, A. R., Campelo, P. H., Bezerra, J. d. A., da Costa Pinto, C., Fonseca Filho, H. D. d., Matos, R. S., Ţălu, Ş., Bagnato, V. S., Inada, N. M., & Sanches, E. A. (2022). Biodefensive Based on Piper nigrum Essential Oil for Controlling of Anopheles aquasalis Larvae: Influence of Temperature (35 °C) and Preservatives. Biomolecules, 12(11), 1711. https://doi.org/10.3390/biom12111711

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop