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Article

3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioink

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Department of Biomedical Engineering, University of Victoria, Victoria, BC V8W 2Y2, Canada
2
Department of Mechanical Engineering, University of Victoria, Victoria, BC V8W 2Y2, Canada
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Division of Medical Sciences, University of Victoria, Victoria, BC V8W 2Y2, Canada
*
Author to whom correspondence should be addressed.
Academic Editor: John T. Connelly
Biomolecules 2021, 11(8), 1250; https://doi.org/10.3390/biom11081250
Received: 1 July 2021 / Revised: 7 August 2021 / Accepted: 18 August 2021 / Published: 21 August 2021
(This article belongs to the Special Issue Extracellular Matrix-Based Bioinks for 3D Bioprinting Applications)
Current treatments for neurodegenerative diseases aim to alleviate the symptoms experienced by patients; however, these treatments do not cure the disease nor prevent further degeneration. Improvements in current disease-modeling and drug-development practices could accelerate effective treatments for neurological diseases. To that end, 3D bioprinting has gained significant attention for engineering tissues in a rapid and reproducible fashion. Additionally, using patient-derived stem cells, which can be reprogrammed to neural-like cells, could generate personalized neural tissues. Here, adipose tissue-derived mesenchymal stem cells (MSCs) were bioprinted using a fibrin-based bioink and the microfluidic RX1 bioprinter. These tissues were cultured for 12 days in the presence of SB431542 (SB), LDN-193189 (LDN), purmorphamine (puro), fibroblast growth factor 8 (FGF8), fibroblast growth factor-basic (bFGF), and brain-derived neurotrophic factor (BDNF) to induce differentiation to dopaminergic neurons (DN). The constructs were analyzed for expression of neural markers, dopamine release, and electrophysiological activity. The cells expressed DN-specific and early neuronal markers (tyrosine hydroxylase (TH) and class III beta-tubulin (TUJ1), respectively) after 12 days of differentiation. Additionally, the tissues exhibited immature electrical signaling after treatment with potassium chloride (KCl). Overall, this work shows the potential of bioprinting engineered neural tissues from patient-derived MSCs, which could serve as an important tool for personalized disease models and drug-screening. View Full-Text
Keywords: 3D bioprinting; fibrin; small molecules; neural tissues; stem cells 3D bioprinting; fibrin; small molecules; neural tissues; stem cells
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MDPI and ACS Style

Restan Perez, M.; Sharma, R.; Masri, N.Z.; Willerth, S.M. 3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioink. Biomolecules 2021, 11, 1250. https://doi.org/10.3390/biom11081250

AMA Style

Restan Perez M, Sharma R, Masri NZ, Willerth SM. 3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioink. Biomolecules. 2021; 11(8):1250. https://doi.org/10.3390/biom11081250

Chicago/Turabian Style

Restan Perez, Milena, Ruchi Sharma, Nadia Z. Masri, and Stephanie M. Willerth. 2021. "3D Bioprinting Mesenchymal Stem Cell-Derived Neural Tissues Using a Fibrin-Based Bioink" Biomolecules 11, no. 8: 1250. https://doi.org/10.3390/biom11081250

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