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Antioxidant and Anti-Inflammatory Properties of Bioavailable Protein Hydrolysates from Lupin-Derived Agri-Waste

1
Department of Medical Biochemistry, Molecular Biology, and Immunology, School of Medicine, Universidad de Sevilla, Av. Sanchez Pizjuan s/n, 41009 Seville, Spain
2
Plant Protein Group, Food and Health Department, Instituto de la Grasa, CSIC. Ctra. de Utrera Km. 1, 41013 Seville, Spain
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Department of Cell Biology, Faculty of Biology, Universidad de Sevilla, Av. Reina Mercedes s/n, 41012 Seville, Spain
4
Cell Biology Unit, Instituto de la Grasa, CSIC. Ctra. de Utrera Km. 1, 41013 Seville, Spain
*
Author to whom correspondence should be addressed.
Academic Editors: Dominico A. Guillén-Sánchez and Valme García-Moreno
Biomolecules 2021, 11(10), 1458; https://doi.org/10.3390/biom11101458
Received: 6 September 2021 / Revised: 29 September 2021 / Accepted: 1 October 2021 / Published: 4 October 2021
(This article belongs to the Special Issue Antioxidants in Food and Waste from the Agri-Food Industry)
Agri-food industries generate several by-products, including protein-rich materials currently treated as waste. Lupine species could be a sustainable alternative source of protein compared to other crops such as soybean or chickpea. Protein hydrolysates contain bioactive peptides that may act positively in disease prevention or treatment. Inflammatory responses and oxidative stress underlie many chronic pathologies and natural treatment approaches have gained attention as an alternative to synthetic pharmaceuticals. Recent studies have shown that lupin protein hydrolysates (LPHs) could be an important source of biopeptides, especially since they demonstrate anti-inflammatory properties. However, due to their possible degradation by digestive and brush-border enzymes, it is not clear whether these peptides can resist intestinal absorption and reach the bloodstream, where they may exert their biological effects. In this work, the in vitro cellular uptake/transport and the anti-inflammatory and antioxidant properties of LPH were investigated in a co-culture system with intestinal epithelial Caco-2 cells and THP-1-derived macrophages. The results indicate that the LPH crosses the human intestinal Caco-2 monolayer and exerts anti-inflammatory activity in macrophages located in the basement area by decreasing mRNA levels and the production of pro-inflammatory cytokines. A remarkable reduction in nitric oxide and ROS in the cell-based system by peptides from LPH was also demonstrated. Our preliminary results point to underexplored protein hydrolysates from food production industries as a novel, natural source of high-value-added biopeptides. View Full-Text
Keywords: agri-lupin waste; by-products; bioeconomy; plant proteins; anti-inflammatory; antioxidant; bioavailability agri-lupin waste; by-products; bioeconomy; plant proteins; anti-inflammatory; antioxidant; bioavailability
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MDPI and ACS Style

Montserrat-de la Paz, S.; Villanueva, A.; Pedroche, J.; Millan, F.; Martin, M.E.; Millan-Linares, M.C. Antioxidant and Anti-Inflammatory Properties of Bioavailable Protein Hydrolysates from Lupin-Derived Agri-Waste. Biomolecules 2021, 11, 1458. https://doi.org/10.3390/biom11101458

AMA Style

Montserrat-de la Paz S, Villanueva A, Pedroche J, Millan F, Martin ME, Millan-Linares MC. Antioxidant and Anti-Inflammatory Properties of Bioavailable Protein Hydrolysates from Lupin-Derived Agri-Waste. Biomolecules. 2021; 11(10):1458. https://doi.org/10.3390/biom11101458

Chicago/Turabian Style

Montserrat-de la Paz, Sergio, Alvaro Villanueva, Justo Pedroche, Francisco Millan, Maria E. Martin, and Maria C. Millan-Linares. 2021. "Antioxidant and Anti-Inflammatory Properties of Bioavailable Protein Hydrolysates from Lupin-Derived Agri-Waste" Biomolecules 11, no. 10: 1458. https://doi.org/10.3390/biom11101458

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