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Open AccessArticle

Different Research Approaches in Unraveling the Venom Proteome of Naja ashei

1
Department of Biotechnology and Bioinformatics, Faculty of Chemistry, Rzeszow University of Technology, Powstańców Warszawy 6, 35-959 Rzeszów, Poland
2
Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland
3
Department of Physiology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovakia
4
Zoological Department, Zoological Garden Košice, Široká 31, 040 06 Košice-Kavečany, Slovakia
5
Department of General Education Subjects, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Kosice, Slovakia
6
Department of Pharmacology and Toxicology, University of Veterinary Medicine and Pharmacy, Komenského 73, 041 81 Košice, Slovakia
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(9), 1282; https://doi.org/10.3390/biom10091282
Received: 31 July 2020 / Revised: 29 August 2020 / Accepted: 3 September 2020 / Published: 5 September 2020
The dynamic development of venomics in recent years has resulted in a significant increase in publicly available proteomic data. The information contained therein is often used for comparisons between different datasets and to draw biological conclusions therefrom. In this article, we aimed to show the possible differences that can arise, in the final results of the proteomic experiment, while using different research workflows. We applied two software solutions (PeptideShaker and MaxQuant) to process data from shotgun LC-MS/MS analysis of Naja ashei venom and collate it with the previous report concerning this species. We were able to provide new information regarding the protein composition of this venom but also present the qualitative and quantitative limitations of currently used proteomic methods. Moreover, we reported a rapid and straightforward technique for the separation of the fraction of proteins from the three-finger toxin family. Our results underline the necessary caution in the interpretation of data based on a comparative analysis of data derived from different studies. View Full-Text
Keywords: snake venom; venomics; absolute protein quantification; label-free shotgun mass spectrometry snake venom; venomics; absolute protein quantification; label-free shotgun mass spectrometry
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MDPI and ACS Style

Hus, K.K.; Marczak, Ł.; Petrilla, V.; Petrillová, M.; Legáth, J.; Bocian, A. Different Research Approaches in Unraveling the Venom Proteome of Naja ashei. Biomolecules 2020, 10, 1282. https://doi.org/10.3390/biom10091282

AMA Style

Hus KK, Marczak Ł, Petrilla V, Petrillová M, Legáth J, Bocian A. Different Research Approaches in Unraveling the Venom Proteome of Naja ashei. Biomolecules. 2020; 10(9):1282. https://doi.org/10.3390/biom10091282

Chicago/Turabian Style

Hus, Konrad K.; Marczak, Łukasz; Petrilla, Vladimír; Petrillová, Monika; Legáth, Jaroslav; Bocian, Aleksandra. 2020. "Different Research Approaches in Unraveling the Venom Proteome of Naja ashei" Biomolecules 10, no. 9: 1282. https://doi.org/10.3390/biom10091282

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