Comparative Characterization of Gluten and Hydrolyzed Wheat Proteins
Abstract
1. Introduction
2. Materials and Methods
2.1. Reagents and Materials
2.2. Determination of Crude Protein Contents
2.3. Stepwise Fractionation According to Solubility
2.4. Reversed-Phase High-Performance Liquid Chromatography (RP-HPLC)
2.5. Gel-Permeation High-Performance Liquid Chromatography (GP-HPLC)
2.6. Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)
2.7. Contents of Free Ammonium
2.8. Statistical Analysis
3. Results
3.1. Determination of Crude Protein Contents
3.2. Stepwise Fractionation According to Solubility and RP-HPLC
3.3. Gel-Permeation High-Performance Liquid Chromatography (GP-HPLC)
3.4. Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)
3.5. Contents of Free Ammonium
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sample | Fraction A | Fraction B | Fraction C | SUM | Protein Content | Free NH4+ |
---|---|---|---|---|---|---|
[mg/g] | [mg/g] | [mg/g] | [mg/g] | [mg/g] | [mg/g] | |
HWP 1 | 400.7 G | <2.3 | <4.7 | 400.7 | 737.4 A,B | 4.12 A,B |
HWP 2 | 569.4 J | 25.9 A | <4.7 | 595.3 | 752.6 A,B | 0.59 A,B |
HWP 3 | 493.6 I | <2.3 | <4.7 | 493.6 | 764.9 A,B | 5.00 B |
HWP 4 | 158.2 E | 237.2 E | 142.5 B | 537.9 | 737.3 A,B | 0.39 A,B |
HWP 5 | 443.4 H | 118.3 B | 15.4 A | 577.1 | 649.9 A | 0.13 A,B |
HWP 6 | 280.0 F | 150.2 C | 162.2 B,C | 592.4 | 750.2 A,B | 0.15 A,B |
HWP 7 | 45.8 D | 423.1 G | 146.5 B | 615.4 | 898.8 B | 0.56 A,B |
G 1 | 18.4 C | 410.9 G | 191.8 C,D | 621.1 | 766.2 A,B | 0.11 A,B |
G 2 | 15.1 B | 509.6 I | 226.6 D | 751.3 | 824.5 A,B | 0.05 A |
G 3 | 14.8 C | 460.6 H | 212.9 D | 688.3 | 778.1 A,B | 0.12 A,B |
G 4 | 11.0 A | 505.6 I | 212.9 D | 729.5 | 855.1 B | 0.05 A |
G 5 | 21.1 C | 394.2 G | 182.4 C | 597.7 | 757.3 A,B | 0.09 A,B |
G 6 | 21.6 C | 427.6 G | 208.9 D | 658.1 | 703.2 A,B | 0.11 A,B |
G 7 | 14.8 B | 321.3 F | 331.3 E | 667.4 | 765.0 A,B | 0.10 A,B |
G 8 | 10.9 A | 183.7 D | 336.9 E | 531.5 | 731.6 A,B | 0.10 A,B |
VC * | 2.24 | 2.29 | 3.13 | - | 6.14 | 5.14 |
Sample | Fraction I | Fraction II | Fraction III | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | 1 | 2 | 3 | 4 | |
[%] | [%] | [%] | [%] | [%] | [%] | [%] | [%] | [%] | [%] | [%] | [%] | |
HWP 1 | - | - | - | 100.0 B | - | - | - | - | - | - | - | - |
HWP 2 | - | 1.5 A | 6.5 D | 92.0 A,B | - | 17.0 B | 32.6 A,B,C | 50.4 C | - | - | - | - |
HWP 3 | - | - | - | 100.0 B | - | - | - | - | - | - | - | - |
HWP 4 | - | 2.3 A,B | 5.0 C | 92.7 A,B | 14.0 C | 16.1 A,B | 26.2 A,B,C | 43.7 B,C | 60.2 A,B | 14.3 A,B | 9.8 A,B | 15.7 A,B |
HWP 5 | 11.6 A | 10.6 B | 11.6 F | 66.2 A | 39.5 F | 14.2 A | 9.7 A | 36.6 A,B,C | 52.0 A,B | 14.3 A,B | 8.3 A,B | 25.4 A,B |
HWP 6 | 7.3 B | 9.7 A,B | 15.6 G | 67.4 A | 31.6 E | 15.2 A,B | 16.4 A,B | 36.8 A,B,C | 63.5 B | 13.4 A,B | 7.8 A | 15.3 A,B |
HWP 7 | - | 4.0 A,B | 8.8 E | 87.2 A,B | 31.0 D,E | 16.9 B | 24.6 A,B,C | 27.5 A | 61.3 A,B | 12.2 A | 11.7 A,B | 14.8 A |
G 7 | - | - | 3.5 B | 96.5 A,B | 11.3 B | 15.1 A,B | 39.5 B,C | 34.1 A,B | 30.7 A,B | 12.1 A | 37.6 A,B | 19.6 A,B |
G 8 | - | - | 2.0 A | 98.0 A,B | 5.8 A | 14.7 A,B | 42.9 C | 36.6 A,B,C | 11.7 A | 15.3 B | 45.0 B | 28.0 B |
CV * [%] | 8.8 | 4.9 | 4.9 | 0.7 | 2.8 | 1.3 | 3.6 | 2.1 | 3.3 | 2.3 | 6.5 | 5.5 |
Sample | Fraction I | Fraction II | Fraction III | ||||||
---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | |
[%] | [%] | [%] | [%] | [%] | [%] | [%] | [%] | [%] | |
HWP 1 | 16.8 B | 33.9 G | 49.3 F | - | - | - | - | - | - |
HWP 2 | 66.3 E | 18.8 F | 14.9 B | 100.0 | - | - | - | - | - |
HWP 3 | 3.9 A | 39.1 H | 57.0 G | - | - | - | - | - | - |
HWP 4 | 75.3 I | 13.7 E | 11.0 A | 100.0 | - | - | 100.0 | - | - |
HWP 5 | 73.7 G,H,I | 11.5 D | 14.8 B | 100.0 | - | - | - | - | - |
HWP 6 | 74.2 H,I | 4.5 B,C | 21.3 C | 100.0 | - | - | 100.0 | - | - |
HWP 7 | 70.5 F | 5.6 C | 23.9 D | 100.0 | - | - | 100.0 | - | - |
G 7 | 53.3 D | 2.5 A | 44.2 E | 100.0 | - | - | 100.0 | - | - |
G 8 | 31.6 C | - | 68.4 H | 100.0 | - | - | 100.0 | - | - |
CV * [%] | 2.0 | 2.9 | 2.6 | 0.0 | - | - | 0.0 | - | - |
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Gabler, A.M.; Scherf, K.A. Comparative Characterization of Gluten and Hydrolyzed Wheat Proteins. Biomolecules 2020, 10, 1227. https://doi.org/10.3390/biom10091227
Gabler AM, Scherf KA. Comparative Characterization of Gluten and Hydrolyzed Wheat Proteins. Biomolecules. 2020; 10(9):1227. https://doi.org/10.3390/biom10091227
Chicago/Turabian StyleGabler, Angelika Miriam, and Katharina Anne Scherf. 2020. "Comparative Characterization of Gluten and Hydrolyzed Wheat Proteins" Biomolecules 10, no. 9: 1227. https://doi.org/10.3390/biom10091227
APA StyleGabler, A. M., & Scherf, K. A. (2020). Comparative Characterization of Gluten and Hydrolyzed Wheat Proteins. Biomolecules, 10(9), 1227. https://doi.org/10.3390/biom10091227