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Review

Bioactive Compounds from Hermetia Illucens Larvae as Natural Ingredients for Cosmetic Application

by
Cíntia Almeida
,
Patrícia Rijo
and
Catarina Rosado
*
CBIOS-Research Center for Biosciences & Health Technologies, Universidade Lusófona, 1749-024 Lisboa, Portugal
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(7), 976; https://doi.org/10.3390/biom10070976
Submission received: 29 May 2020 / Revised: 22 June 2020 / Accepted: 24 June 2020 / Published: 29 June 2020
(This article belongs to the Special Issue Selected Papers from Bio.Natural Meeting 2019)
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Abstract

:
Due to the sustainable organic matter bioconversion process used as substrate for its development, the Hermetia illucens (Linnaeus) larvae biomass is considered a source of compounds with high aggregate value and quite a promising market. The materials that can be extracted from H. illucens larvae have opened the door to a diverse new field of ingredients, mainly for the feed and food industry, but also with potential applicability in cosmetics. In this review we succinctly describe the larval development and rearing cycle, the main compounds identified from different types of extractions, their bioactivities and focus on possible applications in cosmetic products. A search was made in the databases PubMed, ScienceDirect and Web of Science with the terms ‘Hermetia illucens’, ‘bioactives’, ‘biochemical composition’ and ‘cosmetics ingredients’, which included 71 articles published since 1994.

Graphical Abstract

1. Introduction

In the last decade a growing demand for natural and renewable sourced ingredients has been noticed, driving different types of industries, such as animal feed, human foods, pharmaceutical and cosmetics to offer the consumer increasingly innovative products.
The sustainable exploitation of natural resources can be further improved if integrated solutions are employed to tackle the problems of overproduction, disposal of solid organic waste and growing demand for natural raw materials. The concept of a closed system of production minimizes residue generation, energetic costs, transportation and greenhouse gas generation [1].
Natural bioactives have been found in nature from diverse sources like plants, animals and different types of microorganisms [2]. Insect mass cultures have become available in recent years as a source for proteins and lipids and are currently considered a promising alternative. There is a sound rationale for this, since insects are highly efficient waste bio converters, induce a low environmental impact, since they emit less climate-damaging greenhouse gases and consume less water, and the risk of zoonosis is reduced [3,4]. Furthermore, they can be fed with organic waste from various sources and can be reared on small surfaces, therefore, making large-scale industrial breeding possible [5].
In this context, the rearing of the black soldier fly (H. illucens) larvae has motivated great interest, and applications in several sectors are already envisioned, especially due to their nutritional value (Figure 1).
H. illucens larvae are seen as a promising option for providing a sustainable feed source of proteins and lipids [6]. This is mainly linked to their high capacity to convert large quantities of organic waste thanks to the variety of enzymes in their digestive tract [7]. However, this material might also become a potential target for the cosmetic sector, since the H. illucens larvae fatty acids profile resembles those of coconut oil or palm kernel oil, and its biomass contains amino acids and enzymes, which may serve as base ingredients for the development of different products [5]. Thus, using a suitable model of production, the black soldier larvae can act as a great biological control and waste management agent [7] and its biomass can provide compounds with high aggregated-value.
The aim of this brief review is to present the biomass composition of H. illucens larvae, describe the properties of the compounds obtained from their extracts, as well as identify possible applications in the development of innovative cosmetic formulations.

2. Hermetia Lllucens Life Cycle and Rearing Process

Hermetia illucens (Linnaeus), known as the Black Soldier Fly (BSF) is an insect belonging to the order of Dipterans, family of Stratiomyidae [8]. As mentioned previously, this species has a high potential for large-scale production and can be efficiently used to convert low value organic waste into high-value compounds. In order to achieve this, the rearing process must ensure conditions that have satisfactory effects on the growth performance and, thus, composition of H. illucens larvae.
In the H. illucens life cycle, the transition from the larval to the adult stage occurs following a passage through a nymph phase, each with different morphologies and life habits. This insect species has a short development life cycle lasting 45 days with four stages- egg (4 days), larval (18 days), prepupal (14 days) and adult (9 days) [6,9,10]. The eggs are small (around 1 mm) and present a color range between white and cream, as shown in Figure 2 [1]. The larval stage (with five phases) has morphological variations characterized by a color change from beige to dark brown, and in the prepupal stage a migration out of the substrate occurs [9]. The larvae can thrive in various kinds of decaying organic matter due to their efficient oral structures, rich intestinal microbiota and high enzymatic activity, which allows them to metabolize molecules such as starches, proteins and lipids [8]. The time spent in the larval stage depends on physical conditions and food availability [9]. Their life cycle and nutritional composition can be influenced by the quality and amount of the rearing substrate [7,11]. Temperature is also a key environmental parameter for larval development and survival, enabling variations in the 20–30 °C range [12]. After completing its larval development, the insect enters the prepupal stage, when it lies motionless, while its cuticle is rigidified and becomes rich in salts of calcium, forming a dark envelope. In this stage the larva empties its digestive tract and no longer needs to feed, relying on the nutrients stored during the larval stage [13]. Using the insect in the prepupal stage might offer two advantages: an empty digestive tract, reducing the risk of carrying pathogenic microorganisms, and the prepupal migrating behavior that offers opportunities for harvesting in a large-scale rearing system [14].
H. illucens larvae are able to feed on an immense variety of organic material. Different substrates have already been applied in their rearing, ranging from manure, chicken feed, vegetable waste, restaurant waste, as well as winery and brewery agro-industrial by-products [15]. Different studies report that the substrate offered has an impact on the development and biochemical composition of the larvae (Table 1), but concentrations of lipids around 30% and protein around 40% of dry weight (DW) are normally obtained [9].

3. Extraction Methods

The extraction process of animal source compounds must be chosen according to the chemical and physical characteristics of the matrix and the product of interest. Given the biochemical composition of insect biomass, extraction methods are based on the solubility of these compounds in aqueous medium or in organic solvents [18].
Many studies have been dedicated to the extraction of the protein, amino acid, and lipid contents from insects. The fat composition range on average 10 to 60% in dry matter between insect’s species and this amount is higher in the larval stages compared to adults [19]. According to the literature, the highest fat content is generally obtained from extraction methods using different types of organic solvents, such as hexane, petroleum ether, methanol and diethyl ether [1,14,18,20,21,22].
Trompa-Souza et al. [18], compared three extraction methods based on water and organic solvents to determine the lipid profile in different insect species. The best yield was achieved using solvents mixture dichloromethane: methanol (Folch method), and the lowest yield was obtained from the aqueous method. In the four insect species studied, the yields using organic solvents-based methods were ranging around 7.5% to 12.9% with the Folch method and 6.0% to 12.7% with the Soxhlet extraction method. Although the yields from aqueous extraction were low (ranging between 1.6% and 7.8%), it should be noted that this method was able to extract omega-3 fatty acids, an important lipid group due to its health benefits. Furthermore, it was observed by these authors that the lipids obtained from methods with organic solvents provide free fatty acids and partial glycerides, which were not extracted by the aqueous method [18]. The presence of these compounds can lead to a future refinement process. Applying the Soxhlet method and using hexane as solvent, Yi et al. [20] achieved a lipid extraction yield ranging between 3.6 % and 16 % from five different species of insects. In order to obtain the lipid fraction present in H. illucens larvae, Janssen et al. [21] used petroleum ether in the extraction process and the Soxhlet system and the fat content obtained was between 21 and 24% DW. This result was significantly different from that achieved by Bosch et al. [23], who obtained lower fat contents for H. illucens larvae, with a yield around 12.8% of DW, using the same solvent and applying the Soxhlet method. In the assessment of the impact of four types of organic substrate for the H. illucens larvae rearing, Spranghers et al. [12] achieved between 336–386 g·kg−1 of ether extract from the dry matter, applying the Soxhlet method with diethyl ether. Although the use of organic solvents and the Soxhlet method can result in good yields, it should be noted that the time consumed by this methodology is around 6 h. Following an alternative route for lipidomics analysis in the H. illucens larvae, Rabani et al. [24] used water and a biological decomposer. It was observed that when comparing the two experimental groups, control and decomposed, the major fatty acids remained the same, but the oleic acid and linoleic acid content decreased to 18% and 16% respectively, whereas lauric acid and palmitic acid increased to 41% and 16%, respectively [24]. Finally, from the review of the literature it can be perceived that the development of useful and sustainable techniques with use of green solvents for extraction of insect compounds has become an important issue in the chemistry of natural products.
Other studies aimed the evaluation of the protein quality and content, as well as nutritional value and amino acid composition of many insects [25,26]. The protein content of insects is highly variable, and many species contain approximately 60% protein on a dry matter basis [27]. In the quantification of proteins from insect biomass, most of the methods use the nitrogen content, applying nitrogen-to-protein conversion factor (Kp) of 6.25 [20,21,28].. The Dumas method has been applied to the quantification of total nitrogen present in the organic matrix, which is combusted at high temperature in an oxygen atmosphere. This method has been considered a better alternative to the protein and nitrogen quantification due to greater accuracy, speed and productivity [29]. Traditionally, many works use the Kjeldahl method for the determination of the protein content, which measures the total organic nitrogen content based in a distillation and titration process [30]. The amount of protein is obtained from multiplying by a conversion factor. The main limitations of this technique are: (i) slow digestion of samples; (ii) underestimation of nitrogen contents; (iii) use of dangerous reagents at high concentrations; (iv) significant volumes of waste requiring neutralization for discarding [31].
The crude protein contents might be higher than the real protein content, since significant amounts of nitrogen are also bound to the exoskeletons as chitin. The techniques related with protein extraction are based on the use of previously frozen or N2-frozen treated larvae. For extracting and obtaining protein fractions of different insect species, Yi et al. [20] applied an ascorbic acid solution, followed by homogenization, filtration and centrifugation processes to separate the fractions. For quantification of the protein, the authors applied the Dumas method and the results showed that the crude proteins present in the insect species ranged from 19–22%, including the chitin nitrogen. A contrasting technique was applied by Janssen et al. [21], blending the frozen larvae in citric acid solution with 0.2 M disodium phosphate buffer. The obtained solutions were centrifuged and the supernatant was filtered and dialyzed at 4 °C. The protein content (quantified by the Dumas method) ranged from 36 to 49% within larvae of the three insect species studied, from the calculated mean value of Kp 4.76 ± 0.09. The protein content estimated in H. illucens larvae is 36% based on dry weight [21]. This value is consistent with that obtained by Diener et al. [9], who obtained results ranging 31.9% to 46.3% of their dry matter (based on the Dumas method) in the estimate of the protein content in larvae. However, higher protein contents, ranging between 37 and 42%, have been reported in the literature [22,32].
In summary, the utilization of materials from BSF larvae or other insects as ingredients for food, feed, pharmaceuticals or cosmetics requires not only their mass production, but also viable, effective, well-yielding and industrially applicable methodologies for obtaining compounds of interest from insect biomass.

4. H. illucens Larvae Bioactives Composition and Cosmetic Applications

Currently, much attention has been directed to research of natural sources of biologically active substances to be used in cosmetic formulations [33]. Increasingly, the cosmetic industry has been challenged to use more natural and sustainable raw materials, since environmental issues have become one of the main factors that determine the growth of this type of products [34]. Moreover, the consumers are increasingly concerned about the safety of the cosmetic formulations, as well as with the composition and origin of the raw materials that make up these products.
As previously stated, insect production is a potential market business because it is possible to obtain inexpensive and bioactive high value compounds from their biomass [35]. Though the idea of cosmetic products based in insects would be repulsive to many consumers, it is reasonable to consider that extracting insect materials and using these as cosmetic ingredients may increase consumer acceptance [18]. As previously explained, the content of compounds obtained from insect biomass varies considerably, and depends on the stage of metamorphosis, origin of the insect and its diet [36]. Apart from H. illucens, studies addressing the potential use in skin care of different types of insects have explored locusts, house crickets [5] and silkworms [37]. As mentioned above, the H. illucens larvae biomass is so rich that depend on their fat content it can even provide sufficient energy for animal or human diet, as well as satisfactory amounts of proteins and amino acid requirements [6,38]. It is also important to highlight the high contents of mono and polyunsaturated fatty acids, polysaccharides and chitin. Furthermore, it contains micronutrients such as copper, iron, magnesium, manganese, phosphorus, selenium and zinc, as well as vitamins [6,39].
In the next sections, the main compounds obtained from H. illucens larvae biomass and their possible applications as cosmetic ingredients will be highlighted.

4.1. Proteins and Peptides

In general, proteins and peptides are used in cosmetic formulations due to their important role in skin homeostasis and because they can help to maintain skin hydration and to decrease the rate of skin aging [40,41]. Collagen and collagen peptides, mainly extracted from animal sources, are two examples of a biomaterials with these cosmetic applications [42].
According to different studies focused on H. illucens larvae as a source of protein for animal feed, this species has a high protein content, regardless the type of substrate that is used in its rearing [14]. However, from the data collected in the literature it can be seen that there are considerable differences amongst the amino acids profiles of larvae reared on different substrates (Table 2). Taking the particularities of each extraction and quantification method into consideration, it can be observed that aspartic acid, glutamic acid, leucine and valine are the predominant amino acids present in H. illucens larvae, regardless of the substrate. Despite not being the most abundant, arginine and glycine are also present in considerable amounts, and these amino acids have a high applicability as cosmetic ingredients. Glycine is naturally present in the skin as part of the Natural Moisturizing Factor (NMF) [43] and it can also be used as a buffering agent. It has been reported that arginine has important holes: protects the skin from free radicals, acts as an antioxidant, boosts collagen production, in cell division and in the healing process [44]. Arginine can also act as a humectant, increasing the stratum corneum hydration. Proline, also present in considerable quantities in some of the mentioned substrates, is an amino acid present in the collagen molecule and which is part of the NMF, acting as a moisturizer [44].
It is well known that insects have a well-developed innate immune system, subdivided into cellular and humoral defense responses [45]. Survival in decomposition habitats requires substances possessing antibiotic activity as a powerful defense against microorganisms. Antimicrobial peptides (AMPs) are natural antibiotics, which have the ability to kill, or inhibit the growth of various microorganisms that mainly attack the cell envelope [47]. Within the potential bioactive substances that can be extracted from the H. illucens larvae, their capacity for high expression of AMPs and other substances possessing activity against drug resistant “superbugs” can also be considered [45,48]. A study on the antibacterial activity against methicillin resistant Staphylococcus aureus (MRSA) suggests that more than one “antibacterial” substances act in synergy to increase the effect [49].
Recent studies reported the antimicrobial activity of a H. illucens larval extract on several Gram-positive and Gram-negative microorganisms. Amongst the classes of AMPs present, Müller et al. [45] found high expression of defensins, cecropins, attacins and diptericins. Furthermore, these authors reported that: (i) the majority of insect defensins act strongly against Gram-positive microorganisms, e.g., Bacillus subtilis or Staphylococcus aureus; (ii) cecropin peptides exhibit a broad antimicrobial activity against Gram-positive and Gram-negative microorganisms as well as against fungi; (iii) attacins are antimicrobial active against Escherichia coli and some other Gram-negative bacteria, e.g., Acinetobacter calcoaceticus and Pseudomonas maltophilia; (iv) diptericins inhibit the growth of the Gram-negative bacteria E. coli and Salmonella typhimurium. Defensins and cathelicidins are currently some of the most widely studied classes of AMPs in the human skin [47].
In addition, AMPs have been found to have potential as antioxidants, anti-inflammatory agents and promote keratinocyte proliferation [47], and the antioxidant activity in relation to photoinduced lipid peroxidation of melanin and ommochromes isolated from H. illucens was determined [50]. However, it is important to stress that a high production of AMPs by the larvae is caused by a pre-infection with pathogenic organisms (to induce the immune response) or through substrates offered for feeding with a high pathogenic load.
In this context, AMPs obtained from H. illucens larvae can be considered substances with interesting biochemical, scientific and commercial properties and this makes them attractive candidates for the development of novel antimicrobials [49]. These could be used as therapeutic ingredients for the treatment of cutaneous diseases related with the skin microbiota and to promote skin health. Additionally, they could be employed as alternative preservatives in cosmetics, since the safety of conventional preservatives (such as parabens) is currently being questioned by consumers. They could be incorporated in topical formulations such as lotions, creams, shampoos, and could therefore be valuable ingredients for the cosmetic industry [51].

4.2. Lipids and Fatty Acids

Lipids are also a main component of insects biomass and, as such, can be a considerable source of fat [20]. Insect oils are rich in saturated and polyunsaturated fatty acids, in addition to commonly found essential fatty acids such as linoleic and α-linolenic acids [52]. The insects lipid composition is also variable according to their species and life stage [18]. In the case of H. illucens, during larval development their lipid content increases from about 13% to 40% before pupation. Furthermore, predictably, the storage of lipids by H. illucens larvae will depend of its dose in feeding, meaning that the higher the lipid content in the growth substrate, the higher the lipid content of larvae [8]. The oil extracted from H. illucens larvae has a high quality and it is consider a byproduct of animal feed production [45].
Based on a comparison of recent studies, it is possible to observe the main fatty acids profile in H. illucens larvae (Figure 3). Undoubtedly, the most abundant is lauric acid representing 36–60% of total fatty acids, followed by palmitic, oleic, myristic and linoleic acids.
The high content of lauric acid in larvae oil is similar to that of coconut oil, and it should also be noted that many of the beneficial properties of this oil have been attributed to this component. A 12-carbon chain fatty acid, lauric acid is biologically active and has been shown to have potent antibacterial and antiviral activities [56]. Medium chain fatty acids have been reported to have the ability to treat severe bacterial infections that are antibiotic resistant [56]. Lauric acid can be converted into monolaurin, which is known to display antiviral, antibacterial, and antiprotozoal activities [54]. Thus, larvae oil seems promising as a possible alternative preservative. In addition, lauric acid and other fatty acids present in the H. illucens larvae lipid fraction can act as emulsifiers and stabilizers of disperse systems, and are commonly used in the production of soaps and cosmetics [54,56]. Verheyen et al. [5] suggest that due to the high concentration of lauric acid in H. illucens oil, its application in skin cosmetic formulations could be enhanced to that of other oils of natural origin. The authors also recommended the need for refining to remove phospholipids and unsuitable free fatty acids, as well as to improve its color and odor characteristics.
As mentioned above, fatty acids are frequently used in cosmetics, thus other components of H. illucens larvae oil can be applied, either isolated or as a lipidic structural complex [57]. Palmitic, oleic and linoleic acid, along with other essential fatty acids have been identified as key components in the human epidermis [58]. Oleic acid activates the lipidic metabolism, restoring the skin barrier and enabling the retention of moisture in the stratum corneum [59]. Myristic acid is used in cosmetic formulations for thickening and stabilizing emulsions, but it can also restore the cutaneous barrier properties and enhance the permeability of active components into the skin [59]. Palmitic acid and its derivatives are used in creams and lotions as emulsifiers and emollients. Linoleic acid has a physiological role in maintaining the water permeability barrier of the skin, since it is a constituent of acylglycosyl ceramides, which play a structural role in the stratum corneum [60]. It has been suggested that linoleic acid deficiency can result in development of dermatitis in adults and children.
Although not highlighted in the graph in Figure 3, omega-6 fatty acids (ω-6 PUFAs) were also identified in the lipid composition of H. illucens biomass. ω-6 PUFAs have shown beneficial effects in cardiovascular diseases and deficit of these essential fatty acids promotes skin inflammation and impairs wound healing [61].
As described above, fatty acids provide an invaluable contribution to the skin barrier. Thus, an increase in the application of fatty acids as formulation ingredients has been noted, not only due to their functional effects (biological activities), but also for their high biocompatibility.

4.3. Polysaccharides

The exoskeleton of insects is structurally composed of chitin, a nitrogen-containing polysaccharide present in the cuticle of H. illucens larvae [8]. After cellulose, chitin is the most abundant natural polysaccharide on Earth [62]. Chitin is an insoluble fiber, a non-toxic and biodegradable linear polymer. It has been considered a new functional biomaterial with potential application in distinct fields. Despite its low solubility, chitin is considered a good material for fibers feedstock. Chitin fibers can be used as absorbable sutures and wound treatment materials, and have been reported to be able to accelerate the rate of wound healing by about 75% [63]. Additionally, recent studies showed that chitin has effects on the innate and adaptive immune-response, including the ability to recruit and activate immune cells and induce cytokines [64]. Chitin is also associated with the defense mechanisms against some parasitic infections and could reduce allergic reactions to certain entities [65].
After a deacetylation process chitin is transformed into chitosan [66]. This polymer has many applications and is commonly used in water purification and wastewater treatment. But its use in other industrial sectors is growing, from agriculture to pharmaceutical, cosmetics and textile. The properties of chitosan are dependent on many structural factors that can be tailored. Molecular weight, the degree of deacetylation, pH and other features have been shown to play an important role in the final biological activity of chitosan [67]. In health products, chitosan is already used as a carrier or a vector for drug controlled release and in vaccines, but there are also reports of its use against tumors, obesity and high cholesterol [65]. As a cosmetic ingredient, chitosan can work as a hydrocolloid, having the peculiarity of being the only cationic gum of natural origin that becomes viscous after neutralization with acids [68]. This property facilitates its interaction with the skin and hair. Chitosan has also the advantage of being compatible with other biologically active components incorporated in cosmetic formulations [62].
Chitin, chitosan and their derivatives have high application in hair care, and are currently used in shampoos, conditioners, hair tonics, among other products. These molecules and hair have opposite electrical charges [68] forming a clear, elastic film, improving hair softness, smoothness, and elasticity. In skin care products, chitosan also has advantages due to its positive electrical charge and high molecular weight, enabling retention of water molecules by hydrogen bonding [62]. These proprieties prevent skin penetration, allowing chitosan to form a superficial film and act as a moisturizing agent in creams, lipsticks and lotions.
In addition to the previously mentioned activities, the ability of chitin, chitosan and their derivatives to eliminate free radicals via scavenging activity in in vitro and in vivo assays has been reported [66,67]. This antioxidant activity can be used in products aiming to protect the skin against damage by oxidative stress, or to prevent the oxidation of other active ingredients in cosmetic formulations, such as essential oils or vitamins [34].
Chitosan has shown an inhibitory effect against bacteria and fungi, thus having applicability as preservative [69,70]. It is suggested that the antibacterial activity could occur via changes in the bacterial membrane permeability, breakdown of the cytoplasmic barrier or the blockage of nutrient transport, resulting in cell lysis. The degree of deacetylation is a factor that enhanced the antibacterial activity, that is, amino groups seem to play a functional and essential role [71]. Chitosan and some derivatives (mainly cationic derivatives) exhibited antimicrobial activity against Gram-positive and Gram-negative bacteria [69,71]. The mechanism of action in bacteria as E. coli is via an electrostatic interaction with the anionic bacterial surface. The binding of lower molecular weight chitosan derivatives with DNA or RNA inhibited Gram-positive bacteria like S. aureus [49].

5. Conclusions

H. illucens larvae extracts are a promising source of bioactive substances and can play an important role in the development of innovative cosmetics. Due to the sustainability of its rearing, mass cultures of these insects seem to be economically viable. The larvae oil and the protein and chitin content have vast cosmetic applications, and these substances are worth considering as high value co-products. The main challenges associated with their use will be linked with the development of methods to isolate the extracted materials on an industrial scale.

Funding

This work was financially supported by Fundação para a Ciência e a Tecnologia (FCT, Portugal), through projects UID/DTP/04567/2019 and UIDB/04567/2020 and by the research grant attributed to C.A. UID/DTP/04567/2019-CBIOS/BI3.

Conflicts of Interest

The authors declare that there is no financial or personal relationship that can be understood as representing a potential conflict of interest.

References

  1. Teixeira Filho, N.P. Devoradores de lixo: Aspectos biológicos, produtivos e nutricionais da mosca soldado Hermetia illucens (L., 1758) (Díptera; Stratiomyidae) em resíduos sólidos orgânicos em Manaus, AM. Master’s Thesis, Universidade Federal do Amazonas, Manaus, Brazil, 2018. [Google Scholar]
  2. Cragg, G.M.; Newman, D.J. Natural products: A continuing source of novel drug leads. Biochim. Biophys. Acta 2013, 1830, 3670–3695. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  3. Van Huis, A. Potential of Insects as Food and Feed in Assuring Food Security. Annu. Rev. Entomol. 2012, 58, 563–583. [Google Scholar] [CrossRef] [PubMed]
  4. Oonincx, D.G.A.B.; van Itterbeeck, J.; Heetkamp, M.J.W.; van den Brand, H.; van Loon, J.J.A.; van Huis, A. An exploration on greenhouse gas and ammonia production by insect species suitable for animal or human consumption. PLoS ONE 2010, 5, e14445. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  5. Verheyen, G.R.; Ooms, T.; Vogels, L.; Vreysen, S.; Bovy, A.; Van Miert, S.; Meersman, F. Insects as an Alternative Source for the Production of Fats for Cosmetics. J. Cosmet. Sci. 2018, 69, 187–202. [Google Scholar]
  6. Van Huis, A.; van Itterbeeck, J.; Klunder, H.; Mertens, E.; Halloran, A.; Muir, G.; Vantomme, P. Future prospects for food and feed security. Food Agric. Organ. United Nations 2013, 171, 178. [Google Scholar]
  7. Tomberlin, J.K.; Sheppard, D.C.; Joyce, J.A. Selected Life-History Traits of Black Soldier Flies (Diptera: Stratiomyidae) Reared on Three Artificial Diets. Ann. Entomol. Soc. Am. 2002, 95, 379–386. [Google Scholar] [CrossRef]
  8. Devic, E.; Fahmi, M.R. Biology of Hermetia illucens. In Technical Handbook of Domestication and Production of diptera Black Soldier Fly (BSF) Hermetia illucens, Stratiomyidae, 1st ed.; Caruso, D., Devic, E., Wayan Subamia, I., Talamond, P., Baras, E., Eds.; PT Penerbit IPB Press: Jakarta, Indonesia, 2013; pp. 3–10. [Google Scholar]
  9. Diener, S.; Zurbrügg, C.; Tockner, K. Conversion of organic material by black soldier fly larvae: Establishing optimal feeding rates. Waste Manag. Res. 2009, 27, 603–610. [Google Scholar] [CrossRef]
  10. Ferrarezi, R.; Cannella, L.; Nassef, A.; Bailey, D. Annual Report 2006: Alternative Sources of Food for Aquaponics in the U.S. Virgin Islands: A Case Study with Black Soldier Flies; University of the Virgin Islands: Charlotte Amalie West, St. Thomas, US Virgin Islands, 2016. [Google Scholar]
  11. Nguyen, T.T.X.; Tomberlin, J.K.; VanLaerhoven, S. Influence of resources on Hermetia illucens (Diptera: Stratiomyidae) larval development. J. Med. Entomol. 2017, 50, 898–906. [Google Scholar] [CrossRef] [Green Version]
  12. Tomberlin, J.K.; Adler, P.H.; Myers, H.M. Development of the Black Soldier Fly (Diptera: Stratiomyidae) in Relation to Temperature: Table 1. Environ. Entomol. 2009, 38, 930–934. [Google Scholar] [CrossRef]
  13. Sheppard, D.C.; Tomberlin, J.K.; Joyce, J.A.; Kiser, B.C.; Sumner, S.M. Rearing Methods for the Black Soldier Fly (Diptera: Stratiomyidae). J. Med. Entomol. 2002, 39, 695–698. [Google Scholar] [CrossRef] [Green Version]
  14. Spranghers, T.; Ottoboni, M.; Klootwijk, C.; Ovyn, A.; Deboosere, S.; De Meulenaer, B.; Michiels, J.; Eeckhout, M.; De Clercq, P.; De Smet, S. Nutritional composition of black soldier fly (Hermetia illucens) prepupae reared on different organic waste substrates. J. Sci. Food Agric. 2017, 97, 2594–2600. [Google Scholar] [CrossRef]
  15. Meneguz, M.; Schiavone, A.; Gai, F.; Dama, A.; Lussiana, C.; Renna, M.; Gasco, L. Effect of rearing substrate on growth performance, waste reduction efficiency and chemical composition of black soldier fly (Hermetia illucens) larvae. J. Sci. Food Agric. 2018, 98, 5776–5784. [Google Scholar] [CrossRef]
  16. Lalander, C.; Diener, S.; Zurbrügg, C.; Vinnerås, B. Effects of feedstock on larval development and process efficiency in waste treatment with black soldier fly (Hermetia illucens). J. Clean. Prod. 2019, 208, 211–219. [Google Scholar] [CrossRef]
  17. Liland, N.S.; Biancarosa, I.; Araujo, P.; Biemans, D.; Bruckner, C.G.; Waagbø, R.; Torstensen, B.E.; Lock, E.J. Modulation of nutrient composition of black soldier fly (Hermetia illucens) larvae by feeding seaweed-enriched media. PLoS ONE 2017, 12, e0183188. [Google Scholar] [CrossRef] [PubMed]
  18. Tzompa-Sosa, D.; Yi, L.; Valenberg, H.J.F.; Boekel, M.; Lakemond, C. Insect lipid profile: Aqueous versus organic solvent-based extraction methods. Food Res. Int. 2014, 62, 1087–1094. [Google Scholar] [CrossRef]
  19. Xiaoming, C.; Ying, F.; Hong, Z.; Zhiyong, C. Review of the nutritive value of edible insects. In Forest Insects as Food: Humans Bite Back; Durst, P.B., Johnson, D.V., Leslie, R.N., Shono, K., Eds.; Food and Agricultural Organization (FAO) of the United Nations: Bangkok, Thailand, 2010; p. 85. [Google Scholar]
  20. Yi, L.; Lakemond, C.M.M.; Sagis, L.M.C.; Eisner-Schadler, V.; van Huis, A.; van Boekel, M.A.J.S. Extraction and characterisation of protein fractions from five insect species. Food Chem. 2013, 141, 3341–3348. [Google Scholar] [CrossRef]
  21. Janssen, R.H.; Vincken, J.P.; Van Den Broek, L.A.M.; Fogliano, V.; Lakemond, C.M.M. Nitrogen-to-Protein Conversion Factors for Three Edible Insects: Tenebrio molitor, Alphitobius diaperinus, and Hermetia illucens. J. Agric. Food Chem. 2017, 65, 2275–2278. [Google Scholar] [CrossRef] [PubMed]
  22. Newton, G.L.; Booram, C.V.; Barker, R.W.; Hale, O.M. Dried Hermetia illucens Larvae Meal as a Supplement for Swine. J. Anim. Sci. 1977, 44, 395–400. [Google Scholar] [CrossRef]
  23. Bosch, G.; Zhang, S.; Oonincx, D.G.A.B.; Hendriks, W.H. Protein quality of insects as potential ingredients for dog and cat foods. J. Nutr. Sci. 2014, 3, e29. [Google Scholar] [CrossRef] [Green Version]
  24. Rabani, V.; Cheatsazan, H.; Davani, S. Proteomics and Lipidomics of Black Soldier Fly (Diptera: Stratiomyidae) and Blow Fly (Diptera: Calliphoridae) Larvae. J. Insect Sci. 2019, 19, 29. [Google Scholar] [CrossRef]
  25. Renault, D.; Bouchereau, A.; Delettre, Y.R.; Hervant, F.; Vernon, P. Changes in free amino acids in Alphitobius diaperinus (Coleoptera: Tenebrionidae) during thermal and food stress. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 2006, 143, 279–285. [Google Scholar] [CrossRef] [PubMed]
  26. Barker, D.; Fitzpatrick, M.P.; Dierenfeld, E.S. Nutrient composition of selected whole invertebrates. Zoo Biol. 1998, 17, 123–134. [Google Scholar] [CrossRef]
  27. Ramos-Elorduy, J. Insects: A sustainable source of food? Ecol. Food Nutr. 1997, 36, 247–276. [Google Scholar] [CrossRef]
  28. Bukkens, S.G.F. The nutritional value of edible insects. Ecol. Food Nutr. 1997, 36, 287–319. [Google Scholar] [CrossRef]
  29. Marcó, A.; Rubio, R.; Compañó, R.; Casals, I. Comparison of the Kjeldahl method and a combustion method for total nitrogen determination in animal feed. Talanta 2002, 57, 1019–1026. [Google Scholar] [CrossRef]
  30. Lynch, J.M.; Barbano, D.M. Kjeldahl nitrogen analysis as a reference method for protein determination in dairy products. J. AOAC Int. 1999, 82, 1389–1398. [Google Scholar] [CrossRef] [Green Version]
  31. Ribeiro, P.D.A. Implementação de análise de nitrogênio total em solo pelo método de Dumas. In Milho e Sorgo-Documentos; Embrapa: Brasília, Brazil, 2010; p. 26. [Google Scholar]
  32. Sánchez-Muros, M.J.; Barroso, F.G.; Manzano-Agugliaro, F. Insect meal as renewable source of food for animal feeding: A review. J. Clean. Prod. 2014, 65, 16–27. [Google Scholar] [CrossRef]
  33. Aguilar-Toalá, J.E.; Hernández-Mendoza, A.; González-Córdova, A.F.; Vallejo-Cordoba, B.; Liceaga, A.M. Potential role of natural bioactive peptides for development of cosmeceutical skin products. Peptides 2019, 122, 170170. [Google Scholar] [CrossRef]
  34. Aranaz, I.; Acosta, N.; Civera, C.; Elorza, B.; Mingo, J.; Castro, C.; Gandía, M.D.L.L.; Caballero, A.H. Cosmetics and cosmeceutical applications of chitin, chitosan and their derivatives. Polymers 2018, 20, 213. [Google Scholar] [CrossRef] [Green Version]
  35. Wang, Y.S.; Shelomi, M. Review of Black Soldier Fly (Hermetia illucens) as Animal Feed and Human Food. Foods 2017, 6, 91. [Google Scholar] [CrossRef] [Green Version]
  36. Finke, M.D.; Oonincx, D. Insects as Food for Insectivores. In Mass Production of Beneficial Organisms; Morales-Ramos, J.A., Rojas, M.G., Shapiro-Ilan, D.I., Eds.; Academic Press: San Diego, CA, USA, 2014; pp. 583–616. [Google Scholar]
  37. Kunz, R.I.; Brancalhão, R.M.C.; Ribeiro, L.D.F.C.; Natali, M.R.M. Silkworm Sericin: Properties and Biomedical Applications. Biomed. Res. Int. 2016, 2016, 1–19. [Google Scholar] [CrossRef] [Green Version]
  38. Kouřimská, L.; Adámková, A. Nutritional and sensory quality of edible insects. NFS J. 2016, 4, 22–26. [Google Scholar] [CrossRef] [Green Version]
  39. Rumpold, B.A.; Schlüter, O.K. Nutritional composition and safety aspects of edible insects. Mol. Nutr. Food Res. 2013, 57, 802–823. [Google Scholar] [CrossRef] [PubMed]
  40. Mohammed, Y.H.; Yamada, M.; Lin, L.L.; Grice, J.E.; Roberts, M.S.; Raphael, A.P.; Benson, H.E.; Prow, T.W. Microneedle enhanced delivery of cosmeceutically relevant peptides in human skin. PLoS ONE 2014, 9, e101956. [Google Scholar] [CrossRef]
  41. Fields, K.; Falla, T.J.; Rodan, K.; Bush, L. Bioactive peptides: Signaling the future. J. Cosmet. Dermatol. 2009, 8, 8–13. [Google Scholar] [CrossRef] [PubMed]
  42. Avila Rodríguez, M.I.; Rodríguez Barroso, L.G.; Sánchez, M.L. Collagen: A review on its sources and potential cosmetic applications. J. Cosmet. Dermatol. 2018, 17, 20–26. [Google Scholar] [CrossRef]
  43. Burnett, C.L.; Heldreth, B.; Bergfeld, W.F.; Belsito, D.V.; Hill, R.A.; Klaassen, C.D.; Liebler, D.C.; Marks, J.G.; Shank, R.C.; Slaga, T.J.; et al. Safety Assessment of α-Amino Acids as Used in Cosmetics. Int. J. Toxicol. 2013, 32, 41S–64S. [Google Scholar] [CrossRef]
  44. Rebello, T. Produtos orgânicos naturais. In Guia de produtos cosméticos; Rebello, T., Ed.; Senac: São Paulo, Brazil, 2019; p. 320. [Google Scholar]
  45. Muller, A.; Wolf, D.; Gutzeit, H.O. The black soldier fly, Hermetia illucens—A promising source for sustainable production of proteins, lipids and bioactive substances. Z. Naturforsch. C. 2017, 72, 351–363. [Google Scholar] [CrossRef]
  46. Finke, M.D. Complete Nutrient Content of Four Species of Feeder Insects. Zoo Biol. 2013, 32, 27–36. [Google Scholar] [CrossRef]
  47. Alencar-Silva, T.; Braga, M.C.; Santana, G.O.S.; Saldanha-Araujo, F.; Pogue, R.; Dias, S.C.; Franco, O.L.; Carvalho, J.L. Breaking the frontiers of cosmetology with antimicrobial peptides. Biotechnol. Adv. 2018, 36, 2019–2031. [Google Scholar] [CrossRef]
  48. Vogel, H.; Müller, A.; Heckel, D.G.; Gutzeit, H.; Vilcinskas, A. Nutritional immunology: Diversification and diet-dependent expression of antimicrobial peptides in the black soldier fly Hermetia illucens. Dev. Comp. Immunol. 2018, 78, 141–148. [Google Scholar] [CrossRef] [PubMed]
  49. Park, S.I.; Chang, B.S.; Yoe, S.M. Detection of antimicrobial substances from larvae of the black soldier fly, Hermetia illucens (Diptera: Stratiomyidae). Entomol. Res. 2014, 44, 58–64. [Google Scholar] [CrossRef]
  50. Ushakova, N.; Dontsov, A.; Sakina, N.; Bastrakov, A.; Ostrovsky, M. Antioxidative Properties of Melanins and Ommochromes from Black Soldier Fly Hermetia illucens. Biomolecules 2019, 9, 408. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  51. Rahnamaeian, M.; Vilcinskas, A. Short antimicrobial peptides as cosmetic ingredients to deter dermatological pathogens. Appl. Microbiol. Biotechnol. 2015, 99, 8847–8855. [Google Scholar] [CrossRef] [Green Version]
  52. Craig Sheppard, D.; Larry Newton, G.; Thompson, S.A.; Savage, S. A value added manure management system using the black soldier fly. Bioresour. Technol. 1994, 50, 275–279. [Google Scholar] [CrossRef]
  53. Li, S.; Ji, H.; Zhang, B.; Tian, J.; Zhou, J.; Yu, H. Influence of black soldier fly (Hermetia illucens) larvae oil on growth performance, body composition, tissue fatty acid composition and lipid deposition in juvenile Jian carp (Cyprinus carpio var. Jian). Aquaculture 2016. [Google Scholar] [CrossRef]
  54. Ushakova, N.A.; Brodskii, E.S.; Kovalenko, A.A.; Bastrakov, A.I.; Kozlova, A.A.; Pavlov, D.S. Characteristics of lipid fractions of larvae of the black soldier fly Hermetia illucens. Dokl. Biochem. Biophys. 2016, 468, 209–212. [Google Scholar] [CrossRef]
  55. Caligiani, A.; Marseglia, A.; Sorci, A.; Bonzanini, F.; Lolli, V.; Maistrello, L.; Sforza, S. Influence of the killing method of the black soldier fly on its lipid composition. Food Res. Int. 2019. [Google Scholar] [CrossRef]
  56. Anzaku, A.A.; Akyala, J.I.; Juliet, A.; Obianuju, E.C. Antibacterial Activity of Lauric Acid on Some Selected Clinical Isolates. Ann. Clin. Lab. Res. 2017, 4, 3173–3177. [Google Scholar] [CrossRef] [Green Version]
  57. Rabasco Alvarez, A.M.; González Rodríguez, M.L. Lipids in pharmaceutical and cosmetic preparations. Grasas y Aceites 2000, 51, 74–96. [Google Scholar] [CrossRef] [Green Version]
  58. Kim, E.J.; Kim, M.K.; Jin, X.J.; Oh, J.H.; Kim, J.E.; Chung, J.H. Skin aging and photoaging alter fatty acids composition, including 11,14,17-eicosatrienoic acid, in the epidermis of human skin. J. Korean Med. Sci. 2010, 25, 980–983. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  59. Mank, V.; Polonska, T. Use of natural oils as bioactive ingredients of cosmetic products. Ukr. Food J. 2016, 5, 281–289. [Google Scholar] [CrossRef]
  60. Sanders, T.A.B. Introduction: The Role of Fats in Human Diet. In Functional Dietary Lipids; Elsevier: Amsterdam, The Netherlands, 2016; pp. 1–20. [Google Scholar]
  61. Rustan, A.C.; Drevon, C.A. Fatty acids: Structures and properties. In Encyclopedia of Life Sciences; Wyley on Line Library: Hoboken, NJ, USA, 2001; pp. 1–7. [Google Scholar]
  62. Dutta, P.K.; Duta, J.; Tripathi, V.S. Chitin and Chitosan: Chemistry, properties and applications. J. Sci. Ind. Res. 2004, 63, 20–31. [Google Scholar]
  63. Le, Y.; Anand, S.C.; Horrocks, A.R. Recent developments in fibres and materials for wound management. Indian J. Fibre Text. Res. 1997, 22, 337–347. [Google Scholar]
  64. Lee, C.G.; Da Silva, C.A.; Lee, J.Y.; Hartl, D.; Elias, J.A. Chitin regulation of immune responses: An old molecule with new roles. Curr. Opin. Immunol. 2008, 20, 684–689. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  65. Muzzarelli, R.A.A. Chitins and chitosans as immunoadjuvants and non-allergenic drug carriers. Mar. Drugs 2010, 8, 292–312. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  66. Aranaz, I.; Mengibar, M.; Harris, R.; Panos, I.; Miralles, B.; Acosta, N.; Galed, G.; Heras, A. Functional Characterization of Chitin and Chitosan. Curr. Chem. Biol. 2009, 3, 203–230. [Google Scholar]
  67. Ngo, D.H.; Kim, S.K. Antioxidant effects of chitin, chitosan, and their derivatives. In Advances in Food and Nutrition Research; Elsevier: Amsterdam, The Netherlands, 2014; Volume 73, pp. 15–31. [Google Scholar]
  68. Senevirathne, M.; Ahn, C.B.; Kim, S.K.; Je, J.Y. Cosmeceutical Applications of Chitosan and Its Derivatives. In Marine Cosmeceuticals: Trends and Prospects; Kim, S.K., Ed.; CRC Press: Boca Raton, FL, USA, 2011; p. 169. [Google Scholar]
  69. Vinsova, J.; Vavrikova, E. Chitosan Derivatives with Antimicrobial, Antitumour and Antioxidant Activities—A Review. Curr. Pharm. Des. 2011, 17, 3596–3607. [Google Scholar] [CrossRef]
  70. Kong, M.; Chen, X.G.; Xing, K.; Park, H.J. Antimicrobial properties of chitosan and mode of action: A state of the art review. Int. J. Food Microbiol. 2010, 144, 51–63. [Google Scholar] [CrossRef]
  71. Chung, Y.C.; Chen, C.Y. Antibacterial characteristics and activity of acid-soluble chitosan. Bioresour. Technol. 2008, 99, 2806–2814. [Google Scholar] [CrossRef]
Biomolecules 10 00976 g001 550
Figure 1. Flowchart of materials obtained from Hermitia illucens larvae and potential industrial applications.
Figure 1. Flowchart of materials obtained from Hermitia illucens larvae and potential industrial applications.
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Biomolecules 10 00976 g002 550
Figure 2. Development process of H. illucens, from eggs to the larvae stage. Source: Adapted from Caruso et al. [8].
Figure 2. Development process of H. illucens, from eggs to the larvae stage. Source: Adapted from Caruso et al. [8].
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Biomolecules 10 00976 g003 550
Figure 3. Composition of main fatty acids in the H. illucens larvae (percentage of oil content) [14,24,53,54,55].
Figure 3. Composition of main fatty acids in the H. illucens larvae (percentage of oil content) [14,24,53,54,55].
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Table
Table 1. Comparison of the biochemical composition of H. illucens larvae reared on different substrates.
Table 1. Comparison of the biochemical composition of H. illucens larvae reared on different substrates.
Substrate TypeLarvae CompositionReferences
Crude ProteinLipidsChitin
1. Chicken feed412 (0.6) a336 (0.4) a62 (2.8) a[14]
2. Vegetable waste399 (0.2) a371 (1.1) a57 (1.8) a
3. Restaurant waste431 (0.6) a386 (2.3) a67 (1.3) a
4. Biogas digestate422 (1.4) a218 (0.5) a56 (1.5) a
5. Poultry feed39.6 ± 0.2 b--[16]
6. Food waste39.2 ± 2.5 b--
7. Fruits and vegetables41.3 ± 1.0 b--
8. Poultry manure41.6 ± 1.5 b--
9. Fruit waste307.5 ± 10.2 d10.04 c56.0 ± 3.9 d[15]
10. Winery by-product344.3 ± 7.6 d73.57 c52.9 ± 9.2 d
11. Brewery by-product529.6 ± 5.2 d82.47 c14.2 ± 6.0 d
13. Seaweed (Ascophyllum nodosum)41.3 ± 1.1 e22.2 ± 0.2 f-[17]
a Means (and coefficients of variation) in g·kg−1 dry matter; b percentage of dry matter: N-total × 6.25; c g·kg−1 dry matter; d obtained using the nitrogen-to-protein conversion factor of 6.25; e percentage, dry weight and nitrogen × 6.25; f percentage, dry weight.
Table
Table 2. Comparison of the amino acid composition of H. illucens larvae reared on different substrates.
Table 2. Comparison of the amino acid composition of H. illucens larvae reared on different substrates.
Amino acidsSubstrates
Chicken Feed a [14]Organic Waste b [45]Cattle Manure c [21]Human Faeces d [16]Undefined e [46]
Alanine25.2 6.23.765.312.2
Arginine20.36.22.251.012.3
Aspartic acid37.810.34.690.016.5
Cysteine2.50.50.16.71.02
Glutamic acid41.912.23.8105.919.7
Glycine22.65.42.959.29.14
Histidine13.64.81.933.05.94
Isoleucine17.24.82.046.77.62
Leucine28.67.73.570.012.1
Lysine23.47.43.461.711.9
Methionine7.60.60.918.53.37
Phenylalanine176.22.244.27.56
Proline22.56.23.357.910.2
Serine16.64.10.143.97.02
Threonine16.44.50.637.66.82
TryptophanNot analyzedNot analyzed0.216.93.00
Tyrosine6.76.02.5108.812.1
Valine24.16.73.465.912.9
a g·kg−1 dry matter; b % dry matter; c g·100g−1 Protein; d g·kg−1 crude protein; and e g·kg−1 frozen matter.

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Almeida, C.; Rijo, P.; Rosado, C. Bioactive Compounds from Hermetia Illucens Larvae as Natural Ingredients for Cosmetic Application. Biomolecules 2020, 10, 976. https://doi.org/10.3390/biom10070976

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Almeida C, Rijo P, Rosado C. Bioactive Compounds from Hermetia Illucens Larvae as Natural Ingredients for Cosmetic Application. Biomolecules. 2020; 10(7):976. https://doi.org/10.3390/biom10070976

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Almeida, Cíntia, Patrícia Rijo, and Catarina Rosado. 2020. "Bioactive Compounds from Hermetia Illucens Larvae as Natural Ingredients for Cosmetic Application" Biomolecules 10, no. 7: 976. https://doi.org/10.3390/biom10070976

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