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Genome-Wide Identification of RNA Silencing-Related Genes and Their Expressional Analysis in Response to Heat Stress in Barley (Hordeum vulgare L.)

1
Department of Plant Biotechnology, Agricultural Biotechnology Institute, National Agricultural Research and Innovation Centre, 2100 Gödöllő, Hungary
2
Department of Plant Sciences and Biotechnology, Georgikon Faculty, University of Pannonia, 8360 Keszthely, Hungary
3
Doctoral School of Biology, Faculty of Natural Sciences, Eötvös Loránd University, 1053 Budapest, Hungary
*
Authors to whom correspondence should be addressed.
Biomolecules 2020, 10(6), 929; https://doi.org/10.3390/biom10060929
Received: 13 May 2020 / Revised: 10 June 2020 / Accepted: 15 June 2020 / Published: 18 June 2020
(This article belongs to the Section Molecular Biology)
Barley (Hordeum vulgare L.) is an economically important crop cultivated in temperate climates all over the world. Adverse environmental factors negatively affect its survival and productivity. RNA silencing is a conserved pathway involved in the regulation of growth, development and stress responses. The key components of RNA silencing are the Dicer-like proteins (DCLs), Argonautes (AGOs) and RNA-dependent RNA polymerases (RDRs). Despite its economic importance, there is no available comprehensive report on barley RNA silencing machinery and its regulation. In this study, we in silico identified five DCL (HvDCL), eleven AGO (HvAGO) and seven RDR (HvRDR) genes in the barley genome. Genomic localization, phylogenetic analysis, domain organization and functional/catalytic motif identification were also performed. To understand the regulation of RNA silencing, we experimentally analysed the transcriptional changes in response to moderate, persistent or gradient heat stress treatments: transcriptional accumulation of siRNA- but not miRNA-based silencing factor was consistently detected. These results suggest that RNA silencing is dynamically regulated and may be involved in the coordination of development and environmental adaptation in barley. In summary, our work provides information about barley RNA silencing components and will be a ground for the selection of candidate factors and in-depth functional/mechanistic analyses. View Full-Text
Keywords: barley; RNA silencing; Dicer-like (DCL); Argonaute (AGO); RNA-dependent RNA polymerase (RDR); heat stress barley; RNA silencing; Dicer-like (DCL); Argonaute (AGO); RNA-dependent RNA polymerase (RDR); heat stress
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Hamar, É.; Szaker, H.M.; Kis, A.; Dalmadi, Á.; Miloro, F.; Szittya, G.; Taller, J.; Gyula, P.; Csorba, T.; Havelda, Z. Genome-Wide Identification of RNA Silencing-Related Genes and Their Expressional Analysis in Response to Heat Stress in Barley (Hordeum vulgare L.). Biomolecules 2020, 10, 929.

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