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Open AccessArticle

1H Nuclear Magnetic Resonance of Pig Seminal Plasma Reveals Intra-Ejaculate Variation in Metabolites

1
Biotechnology of Animal and Human Reproduction (TechnoSperm), Institute of Food and Agricultural Technology, University of Girona, E-17003 Girona, Spain
2
Unit of Cell Biology, Department of Biology, Faculty of Sciences, University of Girona, E-17003 Girona, Spain
3
Theoretical and Computational Ecology Group, Centre for Advanced Studies of Blanes (CEAB), Consejo Superior de Investigaciones Científicas (CSIC), E-17300 Girona, Spain
4
NMR Facility, Research Technical Services (STR), University of Girona, E-17003 Girona, Spain
5
Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Murcia, E-30100 Murcia, Spain
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Biomolecules 2020, 10(6), 906; https://doi.org/10.3390/biom10060906
Received: 14 May 2020 / Revised: 4 June 2020 / Accepted: 10 June 2020 / Published: 15 June 2020
(This article belongs to the Section Molecular Pathology)
In pigs, ejaculate is expelled in fractions, mainly the sperm-rich fraction (SRF) and the post-SRF (PSRF), which differ in both sperm content and origin. In addition, intra-ejaculate variability between fractions in terms of sperm reproductive characteristics has been previously reported, the highest sperm quality being observed in the first 10 mL of the SRF (SRF-P1). As seminal plasma (SP) composition has been purported to influence sperm physiology, the aim of this study was to profile pig SP metabolite composition and to find putative differences between the ejaculate portions (SRF-P1, the rest of SRF [SRF-P2], PSRF) and entire ejaculate (EE). To this end, ejaculates (n = 8, one per boar) were collected in fractions and SP was analyzed using 1H Nuclear Magnetic Resonance spectroscopy. We identified 19 metabolites present in all ejaculate portions and the EE, and reported correlations between the metabolites. Additionally, and for the first time in mammals, we found intra-ejaculate variability in the SP metabolites, observing different relative abundances in choline, glycerophosphocholine and glycine. Regarding their influence in sperm physiology, we hypothesize that these metabolites may explain the specific reproductive characteristics of each ejaculate portion. Finally, the reported SP metabolites could serve as a first steppingstone in the study of quality, functionality, and fertility biomarkers. View Full-Text
Keywords: nuclear magnetic resonance; seminal plasma; metabolites; pig; intra-ejaculate variability nuclear magnetic resonance; seminal plasma; metabolites; pig; intra-ejaculate variability
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Mateo-Otero, Y.; Fernández-López, P.; Gil-Caballero, S.; Fernandez-Fuertes, B.; Bonet, S.; Barranco, I.; Yeste, M. 1H Nuclear Magnetic Resonance of Pig Seminal Plasma Reveals Intra-Ejaculate Variation in Metabolites. Biomolecules 2020, 10, 906.

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