Next Article in Journal / Special Issue
Human Telomerase RNA: Telomerase Component or More?
Previous Article in Journal
Yeast Sphingolipid-Enriched Domains and Membrane Compartments in the Absence of Mannosyldiinositolphosphorylceramide
Previous Article in Special Issue
A Day in the Life of the Exon Junction Complex
Article

Crystal Structure of a Variant PAM2 Motif of LARP4B Bound to the MLLE Domain of PABPC1

Department of Biochemistry, Theodor Boveri Institute, Biocenter of the University of Würzburg, 97070 Würzburg, Germany
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(6), 872; https://doi.org/10.3390/biom10060872
Received: 3 April 2020 / Revised: 31 May 2020 / Accepted: 4 June 2020 / Published: 6 June 2020
(This article belongs to the Special Issue Ribonucleoprotein Particles (RNPs): From Structure to Function)
Eukaryotic cells determine the protein output of their genetic program by regulating mRNA transcription, localization, translation and turnover rates. This regulation is accomplished by an ensemble of RNA-binding proteins (RBPs) that bind to any given mRNA, thus forming mRNPs. Poly(A) binding proteins (PABPs) are prominent members of virtually all mRNPs that possess poly(A) tails. They serve as multifunctional scaffolds, allowing the recruitment of diverse factors containing a poly(A)-interacting motif (PAM) into mRNPs. We present the crystal structure of the variant PAM motif (termed PAM2w) in the N-terminal part of the positive translation factor LARP4B, which binds to the MLLE domain of the poly(A) binding protein C1 cytoplasmic 1 (PABPC1). The structural analysis, along with mutational studies in vitro and in vivo, uncovered a new mode of interaction between PAM2 motifs and MLLE domains. View Full-Text
Keywords: PAM2w; PAM2; PABC1; MLLE domain; PABP; Poly(A) binding protein PAM2w; PAM2; PABC1; MLLE domain; PABP; Poly(A) binding protein
Show Figures

Figure 1

MDPI and ACS Style

Grimm, C.; Pelz, J.-P.; Schneider, C.; Schäffler, K.; Fischer, U. Crystal Structure of a Variant PAM2 Motif of LARP4B Bound to the MLLE Domain of PABPC1. Biomolecules 2020, 10, 872. https://doi.org/10.3390/biom10060872

AMA Style

Grimm C, Pelz J-P, Schneider C, Schäffler K, Fischer U. Crystal Structure of a Variant PAM2 Motif of LARP4B Bound to the MLLE Domain of PABPC1. Biomolecules. 2020; 10(6):872. https://doi.org/10.3390/biom10060872

Chicago/Turabian Style

Grimm, Clemens, Jann-Patrick Pelz, Cornelius Schneider, Katrin Schäffler, and Utz Fischer. 2020. "Crystal Structure of a Variant PAM2 Motif of LARP4B Bound to the MLLE Domain of PABPC1" Biomolecules 10, no. 6: 872. https://doi.org/10.3390/biom10060872

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop