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Peer-Review Record

Chitosan Nanocomplexes for the Delivery of ENaC Antisense Oligonucleotides to Airway Epithelial Cells

Biomolecules 2020, 10(4), 553; https://doi.org/10.3390/biom10040553
by A. Katharina Kolonko 1,*, Nadine Bangel-Ruland 1, Francisco M. Goycoolea 2 and Wolf-Michael Weber 1
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Biomolecules 2020, 10(4), 553; https://doi.org/10.3390/biom10040553
Submission received: 3 March 2020 / Revised: 31 March 2020 / Accepted: 4 April 2020 / Published: 5 April 2020

Round 1

Reviewer 1 Report

See the attachment

Comments for author File: Comments.pdf

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Reviewer 2 Report

The manuscript entitled “Chitosan nanocomplexes for the delivery of ENaC antisense oligonucleotides to airway epithelial cells” by A. Katharina Kolonko, Nadine Bangel-Ruland, Francisco M. Goycoolea and Wolf-Michael Weber reports development of a chitosan-based antisense oligonucleotide against ENaC for cystic fibrosis (CF) treatment.

Although CF is a devastating disease and the approach of using antisense oligonucleotides is very interesting, the authors have chosen to test their agent in H441 cells which are originally derived from a papillary adenocarcinoma of the lung and are rather used as a model for cell types of the distal lung. However, the pathophysiology of CF is strongly dependent on the dysregulated mucociliary clearance observed more proximally in the lung, as the authors nicely mention in the manuscript. I believe it would be beneficial to mention this in their discussion with possible measures which can be taken in future studies.

Author Response

Please see the attachment.

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

Accept

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