Reduced Local Response to Corticosteroids in Eosinophilic Chronic Rhinosinusitis with Asthma
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Preparation
2.2. Corticosteroid Sensitivity
2.3. Quantitative RT-PCR
2.4. Immunofluorescence Staining
2.5. In-Cell Western Assay
2.6. Cell lysis, Immunoprecipitation, and Western Blotting
2.7. Phosphatase Activity
2.8. RNA Interference
2.9. Transfection
2.10. Statistical Analysis
3. Results
3.1. Local Responses to Corticosteroid are Reduced in ECRS Patients with Asthma
3.2. Decreased PP2A in Eosinophilic Inflammation Leads to Reduced GR Nuclear Translocation
3.3. Eosinophilic Inflammatory Mediators are Increased in the Epithelial Cells of Nasal Polyps
3.4. PP2A may Present a Promising Therapeutic Target for Tocal Corticosteroid Resistance
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kobayashi, Y.; Kanda, A.; Yun, Y.; Bui, D.V.; Suzuki, K.; Sawada, S.; Asako, M.; Iwai, H. Reduced Local Response to Corticosteroids in Eosinophilic Chronic Rhinosinusitis with Asthma. Biomolecules 2020, 10, 326. https://doi.org/10.3390/biom10020326
Kobayashi Y, Kanda A, Yun Y, Bui DV, Suzuki K, Sawada S, Asako M, Iwai H. Reduced Local Response to Corticosteroids in Eosinophilic Chronic Rhinosinusitis with Asthma. Biomolecules. 2020; 10(2):326. https://doi.org/10.3390/biom10020326
Chicago/Turabian StyleKobayashi, Yoshiki, Akira Kanda, Yasutaka Yun, Dan Van Bui, Kensuke Suzuki, Shunsuke Sawada, Mikiya Asako, and Hiroshi Iwai. 2020. "Reduced Local Response to Corticosteroids in Eosinophilic Chronic Rhinosinusitis with Asthma" Biomolecules 10, no. 2: 326. https://doi.org/10.3390/biom10020326