Next Article in Journal
Recent Advances in Allogeneic CAR-T Cells
Previous Article in Journal
Reviewing the Limitations of Adult Mammalian Cardiac Regeneration: Noncoding RNAs as Regulators of Cardiomyogenesis
Previous Article in Special Issue
Cell Penetrating Peptide as a High Safety Anti-Inflammation Ingredient for Cosmetic Applications
Open AccessArticle

Hemoglobin Reassembly of Antimicrobial Fragments from the Midgut of Triatoma infestans

Laboratory of Applied Toxinology, Butantan Institute, São Paulo, SP CEP 05503-900, Brazil
Post-Graduation Program Interunits in Biotechnology, USP/IPT/IBU, São Paulo, SP CEP 05508-900, Brazil
Author to whom correspondence should be addressed.
Current Address: Butantan Institute, Special Laboratory of Toxinology, (LET/CeTICS), Av. Vital Brasil, 1500, São Paulo CEP 05503-900, Brazil.
Biomolecules 2020, 10(2), 261;
Received: 22 November 2019 / Revised: 16 January 2020 / Accepted: 28 January 2020 / Published: 10 February 2020
(This article belongs to the Special Issue Peptides: Molecular and Biotechnological Aspects)
Hemoglobin is one of the most important molecules of the human body. Beyond its physiological activity, hemoglobins are able to inhibit the growth of several microorganisms. Since 1999, studies have reported that antimicrobial peptides can be produced by blood-feeding insects through hemoglobin digestion, and it has been reported that Triatoma infestans can generate an antimicrobial fragment from human fibrinopeptide. Thus T. infestans intestinal content was analyzed through Reverse Phase High-Performance Liquid Chromatography (RP-HPLC), the eluted fractions were tested against Micrococcus luteus, Escherichia coli and Staphylococcus aureus, and the active fractions submitted to mass spectrometry. The data obtained were compared to hemoglobin databases to verify the presence of hemoglobin-derived fragments. Ten fractions eluted from chromatography presented antimicrobial activity, and when analyzed through mass spectrometry revealed the presence of 8 murine hemoglobin α-chain fragments and 24 fragments from murine hemoglobin β fragments. Through the compilation of the fragments is possible to obtain over 67% coverage of both sequences. Part of the amino acid sequences corresponds to the sequences already identified on other intestinal contents of arthropods, and are highly conserved between the blood of other wild animals that are the most common intermediate hosts of Chagas’ disease in Brazil and some of the main natural blood source for triatomines.
Keywords: Triatoma infestans; antimicrobial fragments; hemoglobin; triatomines Triatoma infestans; antimicrobial fragments; hemoglobin; triatomines
MDPI and ACS Style

Diniz, L.C.L.; da Silva Junior, P.I. Hemoglobin Reassembly of Antimicrobial Fragments from the Midgut of Triatoma infestans. Biomolecules 2020, 10, 261.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Back to TopTop