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Article

Reconsideration of the Semaphorin-3A Binding Motif Found in Chondroitin Sulfate Using Galnac4s-6st-Knockout Mice

1
Laboratory of Biochemistry, Kobe Pharmaceutical University, Kobe 658-8558, Japan
2
Faculty of Agriculture, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan
3
Department of Agricultural, Life and Environmental Sciences, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan
4
Multidisciplinary Pain Center, Aichi Medical University, Yazako, Nagakute, Aichi 480-1195, Japan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this manuscript.
Biomolecules 2020, 10(11), 1499; https://doi.org/10.3390/biom10111499
Received: 30 September 2020 / Revised: 26 October 2020 / Accepted: 28 October 2020 / Published: 30 October 2020
(This article belongs to the Special Issue Structural and Functional Approach to the Glycan Diversity)
The chondroitin sulfate (CS)-rich dense extracellular matrix surrounding neuron cell bodies and proximal dendrites in a mesh-like structure is called a perineuronal net (PNN). CS chains in PNNs control neuronal plasticity by binding to PNN effectors, semaphorin-3A (Sema3A) and orthodenticle homeobox 2. Sema3A recognizes CS-containing type-E disaccharide units (sulfated at O-4 and O-6 of N-acetylgalactosamine). Type-E disaccharide units are synthesized by N-acetylgalactosamine 4-sulfate 6-O-sulfotransferase (GalNAc4S-6ST). In this study, we demonstrated that Sema3A accumulates in the PNNs surrounding parvalbumin cells, even in mice deficient in GalNAc4S-6ST. In addition, there were no differences in the number and structure of PNNs visualized by Cat316 antibody and Wisteria floribunda lectin, which recognize CS chains, between wild type and GalNAc4S-6ST knockout mice. Therefore, we re-examined the Sema3A binding motif found in CS chains using chemically synthesized CS tetrasaccharides. As a result, we found that non-sulfated GalNAc residues at the non-reducing termini of CS chains are required for the binding of Sema3A. View Full-Text
Keywords: chondroitin sulfate; proteoglycans; perineuronal net; extracellular matrix chondroitin sulfate; proteoglycans; perineuronal net; extracellular matrix
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MDPI and ACS Style

Nadanaka, S.; Miyata, S.; Yaqiang, B.; Tamura, J.-i.; Habuchi, O.; Kitagawa, H. Reconsideration of the Semaphorin-3A Binding Motif Found in Chondroitin Sulfate Using Galnac4s-6st-Knockout Mice. Biomolecules 2020, 10, 1499. https://doi.org/10.3390/biom10111499

AMA Style

Nadanaka S, Miyata S, Yaqiang B, Tamura J-i, Habuchi O, Kitagawa H. Reconsideration of the Semaphorin-3A Binding Motif Found in Chondroitin Sulfate Using Galnac4s-6st-Knockout Mice. Biomolecules. 2020; 10(11):1499. https://doi.org/10.3390/biom10111499

Chicago/Turabian Style

Nadanaka, Satomi, Shinji Miyata, Bai Yaqiang, Jun-ichi Tamura, Osami Habuchi, and Hiroshi Kitagawa. 2020. "Reconsideration of the Semaphorin-3A Binding Motif Found in Chondroitin Sulfate Using Galnac4s-6st-Knockout Mice" Biomolecules 10, no. 11: 1499. https://doi.org/10.3390/biom10111499

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