Next Article in Journal
Dual Effects of Beta-Hydroxy-Beta-Methylbutyrate (HMB) on Amino Acid, Energy, and Protein Metabolism in the Liver and Muscles of Rats with Streptozotocin-Induced Type 1 Diabetes
Previous Article in Journal
Targeting P53 as a Future Strategy to Overcome Gemcitabine Resistance in Biliary Tract Cancers
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biomolecules
Volume 10
Issue 11
10.3390/biom10111473
biomolecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

New Antibacterial Peptides from the Freshwater Mollusk Pomacea poeyana (Pilsbry, 1927)

by
Melaine González García
1,
Armando Rodríguez
2,3,
Annia Alba
4,
Antonio A. Vázquez
4,
Fidel E. Morales Vicente
5,6,
Julio Pérez-Erviti
1,
Barbara Spellerberg
7,
Steffen Stenger
7,
Mark Grieshober
7,
Carina Conzelmann
8,
Jan Münch
8,
Heinz Raber
9,
Dennis Kubiczek
9,
Frank Rosenau
9,
Sebastian Wiese
3,
Ludger Ständker
2,* and
Anselmo Otero-González
1,*
1
Center for Protein Studies, Faculty of Biology, University of Havana, 25 street, 10400 Havana, Cuba
2
Core Facility for Functional Peptidomics, Faculty of Medicine, Ulm University, 89081 Ulm, Germany
3
Core Unit of Mass Spectrometry and Proteomics, Faculty of Medicine, Ulm University, 89081 Ulm, Germany
4
Reference Center for Research and Diagnosis, Pedro Kourí Institute for Tropical Medicine, 11400 Havana, Cuba
5
General Chemistry Department, Faculty of Chemistry, University of Havana, Zapata y G, 10400 Havana, Cuba
6
Synthetic Peptides Group, Center for Genetic Engineering and Biotechnology, P.O. Box 6162, 10600 Havana, Cuba
7
Institute of Medical Microbiology and Hygiene, University Hospital Ulm, 89081 Ulm, Germany
8
Institute of Molecular Virology, Ulm University, Meyerhofstrasse 1, 89081 Ulm, Germany
9
Institute of Pharmaceutical Biotechnology, Ulm University, 89081 Ulm, Germany
*
Authors to whom correspondence should be addressed.
Biomolecules 2020, 10(11), 1473; https://doi.org/10.3390/biom10111473
Submission received: 3 September 2020 / Revised: 19 October 2020 / Accepted: 20 October 2020 / Published: 23 October 2020
(This article belongs to the Section Natural and Bio-derived Molecules)
Browse Figures
Review Reports Versions Notes

Abstract

Antimicrobial peptides (AMPs) are biomolecules with antimicrobial activity against a broad group of pathogens. In the past few decades, AMPs have represented an important alternative for the treatment of infectious diseases. Their isolation from natural sources has been widely investigated. In this sense, mollusks are promising organisms for the identification of AMPs given that their immune system mainly relies on innate response. In this report, we characterized the peptide fraction of the Cuban freshwater snail Pomacea poeyana (Pilsbry, 1927) and identified 37 different peptides by nanoLC-ESI-MS-MS technology. From these peptide sequences, using bioinformatic prediction tools, we discovered two potential antimicrobial peptides named Pom-1 (KCAGSIAWAIGSGLFGGAKLIKIKKYIAELGGLQ) and Pom-2 (KEIERAGQRIRDAIISAAPAVETLAQAQKIIKGG). Database search revealed that Pom-1 is a fragment of Closticin 574 previously isolated from the bacteria Clostridium tyrobutyrium, and Pom-2 is a fragment of cecropin D-like peptide first isolated from Galleria mellonella hemolymph. These sequences were chemically synthesized and evaluated against different human pathogens. Interestingly, structural predictions of both peptides in the presence of micelles showed models that comprise two alpha helices joined by a short loop. The CD spectra analysis of Pom-1 and Pom-2 in water showed for both structures a high random coil content, a certain content of α-helix and a low β-sheet content. Like other described AMPs displaying a disordered structure in water, the peptides may adopt a helical conformation in presence of bacterial membranes. In antimicrobial assays, Pom-1 demonstrated high activity against the Gram-negative bacteria Pseudomonas aeruginosa and moderate activity against Klebsiella pneumoniae and Listeria monocytogenes. Neither of the two peptides showed antifungal action. Pom-1 moderately inhibits Zika Virus infection but slightly enhances HIV-1 infectivion in vitro. The evaluation of cell toxicity on primary human macrophages did not show toxicity on THP-1 cells, although slight overall toxicity was observed in high concentrations of Pom-1. We assume that both peptides may play a key role in innate defense of P. poeyana and represent promising antimicrobial candidates for humans.
Keywords: antimicrobial peptides; Pomacea poeyana; antibacterial activity antimicrobial peptides; Pomacea poeyana; antibacterial activity

Share and Cite

MDPI and ACS Style

González García, M.; Rodríguez, A.; Alba, A.; Vázquez, A.A.; Morales Vicente, F.E.; Pérez-Erviti, J.; Spellerberg, B.; Stenger, S.; Grieshober, M.; Conzelmann, C.; et al. New Antibacterial Peptides from the Freshwater Mollusk Pomacea poeyana (Pilsbry, 1927). Biomolecules 2020, 10, 1473. https://doi.org/10.3390/biom10111473

AMA Style

González García M, Rodríguez A, Alba A, Vázquez AA, Morales Vicente FE, Pérez-Erviti J, Spellerberg B, Stenger S, Grieshober M, Conzelmann C, et al. New Antibacterial Peptides from the Freshwater Mollusk Pomacea poeyana (Pilsbry, 1927). Biomolecules. 2020; 10(11):1473. https://doi.org/10.3390/biom10111473

Chicago/Turabian Style

González García, Melaine, Armando Rodríguez, Annia Alba, Antonio A. Vázquez, Fidel E. Morales Vicente, Julio Pérez-Erviti, Barbara Spellerberg, Steffen Stenger, Mark Grieshober, Carina Conzelmann, and et al. 2020. "New Antibacterial Peptides from the Freshwater Mollusk Pomacea poeyana (Pilsbry, 1927)" Biomolecules 10, no. 11: 1473. https://doi.org/10.3390/biom10111473

APA Style

González García, M., Rodríguez, A., Alba, A., Vázquez, A. A., Morales Vicente, F. E., Pérez-Erviti, J., Spellerberg, B., Stenger, S., Grieshober, M., Conzelmann, C., Münch, J., Raber, H., Kubiczek, D., Rosenau, F., Wiese, S., Ständker, L., & Otero-González, A. (2020). New Antibacterial Peptides from the Freshwater Mollusk Pomacea poeyana (Pilsbry, 1927). Biomolecules, 10(11), 1473. https://doi.org/10.3390/biom10111473

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop