Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels
Abstract
:1. Introduction
2. Materials and Methods
2.1. Physicochemical Characterization
2.1.1. Preparation of Silk Fibroin Solution
2.1.2. Horseradish Peroxidase to Induce Ty–GG/SF Hydrogel Formation
2.1.3. Analysis of the Secondary Protein Structure of Silk Fibroin
2.1.4. Water Uptake and Enzymatic Degradation of Ty–GG/SF Hydrogels
2.1.5. Gelation Time, Injectability and Rheological Properties of the Ty–GG/SF Hydrogels
2.1.6. Betamethasone Release Studies of the Ty–GG/SF Hydrogels
2.2. In Vitro Studies
2.2.1. Chondrogenic Cell Isolation
2.2.2. Cell Culture
2.2.3. Metabolic Activity
2.2.4. DNA Quantification
2.2.5. Fluorescence Microscopy
2.2.6. The Therapeutic Efficacy of Ty–GG/SF with Betamethasone Encapsulated
2.3. Statistical Analysis
3. Results and Discussion
3.1. Physicochemical Characterization
3.2. In Vitro Studies
4. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Ty–GG | SF | HRP | H2O2 | |
---|---|---|---|---|
C1 | 83.5 µL | 83.5 µL | 16.6 µL | 10.83 µL |
C2 | 83.5 µL | 83.5 µL | 18.3 µL | 15 µL |
C3 | 83.5 µL | 83.5 µL | 20 µL | 13.3 µL |
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Oliveira, I.M.; Gonçalves, C.; Shin, M.E.; Lee, S.; Reis, R.L.; Khang, G.; Oliveira, J.M. Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels. Biomolecules 2020, 10, 1456. https://doi.org/10.3390/biom10101456
Oliveira IM, Gonçalves C, Shin ME, Lee S, Reis RL, Khang G, Oliveira JM. Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels. Biomolecules. 2020; 10(10):1456. https://doi.org/10.3390/biom10101456
Chicago/Turabian StyleOliveira, Isabel Matos, Cristiana Gonçalves, Myeong Eun Shin, Sumi Lee, Rui Luis Reis, Gilson Khang, and Joaquim Miguel Oliveira. 2020. "Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels" Biomolecules 10, no. 10: 1456. https://doi.org/10.3390/biom10101456