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Open AccessArticle

Serine Protease Inhibitors—New Molecules for Modification of Polymeric Biomaterials

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Department of Biochemistry and Biotechnology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland
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Department of Functional Anatomy and Cytobiology, Maria Curie-Skłodowska University, Akademicka 19, 20-033 Lublin, Poland
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Laboratory of X-Ray Optics, Centre of Interdisciplinary Research, The John Paul II Catholic University of Lublin, Konstantynów 1, 20-708 Lublin, Poland
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(1), 82; https://doi.org/10.3390/biom10010082
Received: 21 November 2019 / Revised: 25 December 2019 / Accepted: 31 December 2019 / Published: 4 January 2020
(This article belongs to the Special Issue New Targets and Strategies in Regenerative Medicine)
Three serine protease inhibitors (AEBSF, soy inhibitor, α1-antitrypsin) were covalently immobilized on the surface of three polymer prostheses with the optimized method. The immobilization efficiency ranged from 11 to 51%, depending on the chosen inhibitor and biomaterial. The highest activity for all inhibitors was observed in the case of immobilization on the surface of the polyester Uni-Graft prosthesis, and the preparations obtained showed high stability in the environment with different pH and temperature values. Modification of the Uni-Graft prosthesis surface with the synthetic AEBSF inhibitor and human α1-antitrypsin inhibited the adhesion and multiplication of Staphylococcus aureus subs. aureus ATCC® 25923TM and Candida albicans from the collection of the Department of Genetics and Microbiology, UMCS. Optical profilometry analysis indicated that, after the immobilization process on the surface of AEBSF-modified Uni-Graft prostheses, there were more structures with a high number of protrusions, while the introduction of modifications with a protein inhibitor led to the smoothing of their surface. View Full-Text
Keywords: covalent immobilization; protease inhibitors; modification of biomaterials covalent immobilization; protease inhibitors; modification of biomaterials
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Szałapata, K.; Osińska-Jaroszuk, M.; Kapral-Piotrowska, J.; Pawlikowska-Pawlęga, B.; Łopucki, R.; Mroczka, R.; Jarosz-Wilkołazka, A. Serine Protease Inhibitors—New Molecules for Modification of Polymeric Biomaterials. Biomolecules 2020, 10, 82.

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