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Back to GroEL-Assisted Protein Folding: GroES Binding-Induced Displacement of Denatured Proteins from GroEL to Bulk Solution

1
Institute of Protein Research, Russian Academy of Sciences, Institutskaya Street 4, 142290 Pushchino, Russia
2
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Ulitsa Miklukho-Maklaya 16/10, GSP-7, 117997 Moscow, Russia
3
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki 5, 142290 Pushchino, Russia
*
Author to whom correspondence should be addressed.
Biomolecules 2020, 10(1), 162; https://doi.org/10.3390/biom10010162
Received: 29 December 2019 / Revised: 16 January 2020 / Accepted: 18 January 2020 / Published: 20 January 2020
The main events in chaperone-assisted protein folding are the binding and ligand-induced release of substrate proteins. Here, we studied the location of denatured proteins previously bound to the GroEL chaperonin resulting from the action of the GroES co-chaperonin in the presence of Mg-ATP. Fluorescein-labeled denatured proteins (α-lactalbumin, lysozyme, serum albumin, and pepsin in the presence of thiol reagents at neutral pH, as well as an early refolding intermediate of malate dehydrogenase) were used to reveal the effect of GroES on their interaction with GroEL. Native electrophoresis has demonstrated that these proteins tend to be released from the GroEL-GroES complex. With the use of biotin- and fluorescein-labeled denatured proteins and streptavidin fused with luciferase aequorin (the so-called streptavidin trap), the presence of denatured proteins in bulk solution after GroES and Mg-ATP addition has been confirmed. The time of GroES-induced dissociation of a denatured protein from the GroEL surface was estimated using the stopped-flow technique and found to be much shorter than the proposed time of the GroEL ATPase cycle. View Full-Text
Keywords: GroEL-assisted protein folding; chaperonins; protein-protein interaction; bioluminescence; fluorescence GroEL-assisted protein folding; chaperonins; protein-protein interaction; bioluminescence; fluorescence
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Marchenkov, V.; Gorokhovatsky, A.; Marchenko, N.; Ivashina, T.; Semisotnov, G. Back to GroEL-Assisted Protein Folding: GroES Binding-Induced Displacement of Denatured Proteins from GroEL to Bulk Solution. Biomolecules 2020, 10, 162.

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