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Open AccessArticle

Transcriptomic Analysis Reveals the Wound Healing Activity of Mussel Myticin C

1
Institute of Marine Research (IIM), CSIC. Eduardo Cabello 6, 36208 Vigo, Spain
2
Instituto de Investigación, Desarrollo e Innovación en Biotecnología Sanitaria de Elche (IDiBE) and Instituto de Biología Molecular y Celular (IBMC), Miguel Hernández University (UMH), 03202 Elche Alicante, Spain
3
Instituto Química-Física “Rocasolano”, CSIC. Serrano 119, 28006 Madrid, Spain
*
Authors to whom correspondence should be addressed.
Biomolecules 2020, 10(1), 133; https://doi.org/10.3390/biom10010133
Received: 11 December 2019 / Revised: 9 January 2020 / Accepted: 10 January 2020 / Published: 14 January 2020
(This article belongs to the Special Issue Marine-Derived Biomolecules)
Myticin C is the most studied antimicrobial peptide in the marine mussel Mytilus galloprovincialis. Although it is constitutively expressed in mussel hemocytes and displays antibacterial, antiviral, and chemotactic functions, recent work has suggested that this molecule is mainly activated after tissue injury. Therefore, the main objective of this work was to characterize the hemocytes’ transcriptomic response after a myticin C treatment, in order to understand the molecular changes induced by this cytokine-like molecule. The transcriptome analysis revealed the modulation of genes related to cellular movement, such as myosin, transgelin, and calponin-like proteins, in agreement with results of functional assays, where an implication of myticin C in the in vitro activation of hemocytes and migration was evidenced. This was also observed in vivo after a tissue injury, when hemocytes, with high concentrations of myticin C, migrated to the damaged area to heal the wound. All these properties allowed us to think about the biotechnological application of these molecules as wound healers. Human keratinocytes and larvae zebrafish models were used to confirm this hypothesis. Accelerated regeneration after a wound or tail fin amputation was observed after treatment with the myticin C peptide, supporting the chemotactic and healing activity of myticin C. View Full-Text
Keywords: Mytilus galloprovincialis; myticin C; hemocytes; chemotaxis; Illumina; wound healing; tissue injury; regeneration Mytilus galloprovincialis; myticin C; hemocytes; chemotaxis; Illumina; wound healing; tissue injury; regeneration
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Rey-Campos, M.; Moreira, R.; Romero, A.; Medina-Gali, R.M.; Novoa, B.; Gasset, M.; Figueras, A. Transcriptomic Analysis Reveals the Wound Healing Activity of Mussel Myticin C. Biomolecules 2020, 10, 133.

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