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Open AccessArticle

Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts

Department of Bioscience and Biotechnology, Konkuk University, Seoul 05029, Korea
Department of Biological Resources Utilization, National Institute of Biological Resources, Environmental Research Complex, Incheon 22755, Korea
Research Institute for Bioactive-Metabolome Network, Konkuk University, Seoul 05029, Korea
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Metabolites 2019, 9(9), 186;
Received: 29 August 2019 / Revised: 10 September 2019 / Accepted: 15 September 2019 / Published: 16 September 2019
(This article belongs to the Special Issue Plant Metabolomics)
Plant species have traditionally been revered for their unparalleled pharmacognostic applications. We outline a non-iterative multi-parallel metabolomic-cum-bioassay-guided methodology toward the functional characterization of ethanol extracts from the Betulaceae family plants (n = 10). We performed mass spectrometry (MS)-based multivariate analyses and bioassay-guided (ABTS antioxidant activity and cytoprotective effects against H2O2-induced cell damage) analyses of SPE fractions. A clearly distinct metabolomic pattern coupled with significantly higher bioactivities was observed for 40% methanol SPE eluate. Further, the 40% SPE eluate was subjected to preparative high-performance liquid chromatography (prep-HPLC) analysis, yielding 72 sub-fractions (1 min−1), with the highest antioxidant activities observed for the 15 min and 31 min sub-fractions. We simultaneously performed hyphenated-MS-based metabolite characterization of bioactive components for both the 40% methanol SPE fraction and its prep-HPLC sub-fraction (15 min and 31 min). Altogether, 19 candidate metabolites were mainly observed to contribute toward the observed bioactivities. In particular, ethyl gallate was mainly observed to affect the antioxidant activities of SPE and prep-HPLC fractions of Alnus firma extracts. We propose an integrated metabolomic-cum-bioassay-guided approach for the expeditious selection and characterization of discriminant metabolites with desired phenotypes or bioactivities. View Full-Text
Keywords: Betulaceae; Alnus firma; mass spectrometry; bioassays; ethyl gallate Betulaceae; Alnus firma; mass spectrometry; bioassays; ethyl gallate
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Lee, S.; Oh, D.-G.; Singh, D.; Lee, H.J.; Kim, G.R.; Lee, S.; Lee, J.S.; Lee, C.H. Untargeted Metabolomics Toward Systematic Characterization of Antioxidant Compounds in Betulaceae Family Plant Extracts. Metabolites 2019, 9, 186.

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