Optimal Regimen of N-Acetylcysteine on Chromium-Induced Renal Cell Damage
Abstract
:1. Introduction
2. Results
2.1. Cr(VI)-Induced Toxicity Results in HK-2 Apoptosis
2.2. NAC Treatment Protects HK-2 from Cr(VI)-Induced ROS
2.3. NAC Treatment Protects HK-2 from Cr(VI)-Induced Cell Death
2.4. NAC Treatment Altered Cr(VI)-Induced Apoptotic Pathways
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. HK-2 Cell Culture
5.2. MTT Assay for Cell Viability
5.3. Annexin V/Propidium Iodide (PI) Staining
5.4. Oxidative Stress Assays
5.5. Apoptotic Assay by Western Blot Analysis
5.6. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Yeh, I.-J.; Wang, T.-Y.; Lin, J.-C.; Lin, T.-J.; Chang, J.-S.; Yen, M.-C.; Liu, Y.-H.; Wu, P.-L.; Chen, F.-W.; Shih, Y.-L.; et al. Optimal Regimen of N-Acetylcysteine on Chromium-Induced Renal Cell Damage. Metabolites 2019, 9, 172. https://doi.org/10.3390/metabo9090172
Yeh I-J, Wang T-Y, Lin J-C, Lin T-J, Chang J-S, Yen M-C, Liu Y-H, Wu P-L, Chen F-W, Shih Y-L, et al. Optimal Regimen of N-Acetylcysteine on Chromium-Induced Renal Cell Damage. Metabolites. 2019; 9(9):172. https://doi.org/10.3390/metabo9090172
Chicago/Turabian StyleYeh, I-Jeng, Tzu-Yi Wang, Jhong-Ching Lin, Tzeng-Jih Lin, Jung-San Chang, Meng-Chi Yen, Yao-Hua Liu, Pei-Lin Wu, Fen-Wei Chen, Yueh-Lun Shih, and et al. 2019. "Optimal Regimen of N-Acetylcysteine on Chromium-Induced Renal Cell Damage" Metabolites 9, no. 9: 172. https://doi.org/10.3390/metabo9090172